Literature DB >> 4216023

Isolation of DNA-membrane complex in Bacillus subtilis.

N Sueoka, J M Hammers.   

Abstract

A DNA-membrane complex of Bacillus subtilis was separated from the bulk of the membrane by CsCl-sucrose double gradient centrifugation. The complex has a CsCl-sucrose buoyant density of 1.35-1.45 g/cm(3). Gel electrophoretic analyses show that the complex has about 5% of the whole membrane proteins and contains proteins unique to the complex as well as a small amount of membrane proteins. DNA in the complex is enriched for a genetic marker close to the origin (pur A16) and for one near the terminus (metB5). Artifactual formation of the complex during the lysate preparation was shown to be unlikely.

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Year:  1974        PMID： 4216023      PMCID： PMC433982          DOI： 10.1073/pnas.71.12.4787

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  26 in total

1.  Evidence for stable attachment of DNA to membrane at the replication origin of Escherichia coli.

Authors:  P Fielding; C F Fox
Journal:  Biochem Biophys Res Commun       Date:  1970-10-09       Impact factor: 3.575

2.  Properties of the growing point region in the bacterial chromosome.

Authors:  D W Smith; P C Hanawalt
Journal:  Biochim Biophys Acta       Date:  1967-12-19

3.  Association between the chromosome and the cytoplasmic membrane in Bacillus subtilis.

Authors:  R W Snyder; F E Young
Journal:  Biochem Biophys Res Commun       Date:  1969-05-08       Impact factor: 3.575

4.  Density gradient system. I. Formation and fractionation of density gradients.

Authors:  R C Leif
Journal:  Anal Biochem       Date:  1968-10-24       Impact factor: 3.365

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Initiation by the DNA-dependent RNA polymerase.

Authors:  D D Anthony; E Zeszotek; D A Goldthwait
Journal:  Proc Natl Acad Sci U S A       Date:  1966-09       Impact factor: 11.205

7.  Membrane attachment of the replication origins of a multifork (dichotomous) chromosome in Bacillus subtilis.

Authors:  M A O'Sullivan; N Sueoka
Journal:  J Mol Biol       Date:  1972-08-21       Impact factor: 5.469

8.  Some unique properties of the deoxyribonucleic acid-bearing portion of the bacterial membrane.

Authors:  J P Ballesta; E Cundliffe; M J Daniels; J L Silverstein; M M Susskind; M Schaechter
Journal:  J Bacteriol       Date:  1972-10       Impact factor: 3.490

9.  Chromosome-membrane association in Bacillus subtilis. I. DNA release from membrane fraction.

Authors:  K Yamaguchi; S Murakami; H Yoshikawa
Journal:  Biochem Biophys Res Commun       Date:  1971-09-17       Impact factor: 3.575

10.  Association of the Bacillus subtilis chromosome with the cell membrane: resolution of free and bound deoxyribonucleic acid on renografin gradients.

Authors:  R D Ivarie; J J Pène
Journal:  J Bacteriol       Date:  1970-11       Impact factor: 3.490
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  34 in total

1.  Selective association of the chromosome with membrane in a stable L-form of Bacillus subtilis.

Authors:  S Horowitz; R J Doyle; F E Young; U N Streips
Journal:  J Bacteriol       Date:  1979-06       Impact factor: 3.490

2.  Membrane enrichment of genetic markers close to the origin and terminus during the deoxyribonucleic acid replication cycle in Bacillus subtilis.

Authors:  J Beeson; N Sueoka
Journal:  J Bacteriol       Date:  1979-09       Impact factor: 3.490

3.  Two membrane sites for DNA synthesis in Pneumococcus.

Authors:  W Firshein
Journal:  Mol Gen Genet       Date:  1976-11-17

4.  Chromosome-membrane association in Bacillus subtilis. IV. Further purification of DNA-membrane complex by using a combination of centrifugation and electrophoresis.

Authors:  H Toyoda; K Yamaguchi; H Yoshikawa
Journal:  J Bacteriol       Date:  1978-11       Impact factor: 3.490

5.  Chromatin and DNA synthesis associated with nuclear membrane in germinating cotton.

Authors:  W F Clay; F R Katterman; P G Bartels
Journal:  Proc Natl Acad Sci U S A       Date:  1975-08       Impact factor: 11.205

6.  Association of the folded chromosome with the cell envelope of Escherichia coli: nature of the membrane-associated DNA.

Authors:  K Drlica; E Burgi; A Worcel
Journal:  J Bacteriol       Date:  1978-06       Impact factor: 3.490

7.  Escherichia coli membrane proteins with an affinity for deoxyribonucleic acid.

Authors:  M Kohiyama; R Kollek; W Goebel; A Kepes
Journal:  J Bacteriol       Date:  1977-02       Impact factor: 3.490

8.  Molecular fate of heterologous bacterial DNA in competent Bacillus subtilis: further characterization of unstable association between donor and recipient DNA and the involvement of the cellular membrane.

Authors:  H P te Riele; G Venema
Journal:  Mol Gen Genet       Date:  1984

9.  In vitro type II binding of chromosomal DNA to membrane in Bacillus subtilis.

Authors:  Y Sato; M McCollum; T McKenzie; J Laffan; A Zuberi; N Sueoka
Journal:  J Bacteriol       Date:  1991-12       Impact factor: 3.490

10.  Independence of deoxyribonucleic acid replication and initiation from membrane fluidity and the supply of unsaturated fatty acids in Escherichia coli.

Authors:  L Thilo; W Vielmetter
Journal:  J Bacteriol       Date:  1976-10       Impact factor: 3.490
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