Literature DB >> 4580564

Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate.

C Filip, G Fletcher, J L Wulff, C F Earhart.   

Abstract

The sensitivity of the outer and cytoplasmic membranes of Escherichia coli to detergent was examined by isopycnic sucrose density gradient centrifugation. Sodium lauryl sarcosinate (Sarkosyl) was found to disrupt the cytoplasmic membrane selectively under conditions in which Triton X-100 and dodecyl sodium sulfate solubilized all membrane protein. These results were verified by gel electrophoresis; membrane proteins solubilized by Sarkosyl were identical to those of the cytoplasmic membrane. The presence of Mg(2+) during treatment with Sarkosyl was found to afford partial protection of the cytoplasmic membrane from dissolution.

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Year:  1973        PMID： 4580564      PMCID： PMC246312          DOI： 10.1128/jb.115.3.717-722.1973

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  17 in total

1.  Purification of bacterial membrane proteins. The use of guanidinium thiocyanate and urea.

Authors:  C Moldow; J Robertson; L Rothfield
Journal:  J Membr Biol       Date:  1972       Impact factor: 1.843

2.  Examination of the protein composition of the cell envelope of Escherichia coli by polyacrylamide gel electrophoresis.

Authors:  C A Schnaitman
Journal:  J Bacteriol       Date:  1970-11       Impact factor: 3.490

3.  Lysis of the cell membrane of Escherichia coli K12 by ionic detergents.

Authors:  C L Woldringh
Journal:  Biochim Biophys Acta       Date:  1970-11-12

4.  The association of host and phage DNA with the membrane of Escherichia coli.

Authors:  C F Earhart
Journal:  Virology       Date:  1970-10       Impact factor: 3.616

5.  A mutation which changes a membrane protein of E. coli.

Authors:  M Inouye; J P Guthrie
Journal:  Proc Natl Acad Sci U S A       Date:  1969-11       Impact factor: 11.205

Review 6.  Structure and biosynthesis of the bacterial cell wall.

Authors:  M J Osborn
Journal:  Annu Rev Biochem       Date:  1969       Impact factor: 23.643

7.  A computer method for analysis of radioactivity data from single and double labeled experiments.

Authors:  M A Yund; E W Yund; F C Kafatos
Journal:  Biochem Biophys Res Commun       Date:  1971-05-21       Impact factor: 3.575

8.  Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli.

Authors:  C A Schnaitman
Journal:  J Bacteriol       Date:  1970-11       Impact factor: 3.490

9.  Some features of the bacterial membrane studied with the aid of a new fractionation technique.

Authors:  M J Daniels
Journal:  Biochem J       Date:  1971-04       Impact factor: 3.857

10.  Attachment of flagellar basal bodies to the cell envelope: specific attachment to the outer, lipopolysaccharide membrane and the cyoplasmic membrane.

Authors:  M L DePamphilis; J Adler
Journal:  J Bacteriol       Date:  1971-01       Impact factor: 3.490
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  399 in total

1.  Characterization of outer membrane proteins in Chlamydia trachomatis LGV serovar L2.

Authors:  R J Tanzer; T P Hatch
Journal:  J Bacteriol       Date:  2001-04       Impact factor: 3.490

2.  Membrane topology of the NixA nickel transporter of Helicobacter pylori: two nickel transport-specific motifs within transmembrane helices II and III.

Authors:  J F Fulkerson; H L Mobley
Journal:  J Bacteriol       Date:  2000-03       Impact factor: 3.490

3.  impA, a gene coding for an inner membrane protein, influences colonial morphology of Actinobacillus actinomycetemcomitans.

Authors:  K P Mintz; P M Fives-Taylor
Journal:  Infect Immun       Date:  2000-12       Impact factor: 3.441

4.  The high-molecular-weight cytochrome c Cyc2 of Acidithiobacillus ferrooxidans is an outer membrane protein.

Authors:  Andrés Yarzábal; Gaël Brasseur; Jeanine Ratouchniak; Karen Lund; Danielle Lemesle-Meunier; John A DeMoss; Violaine Bonnefoy
Journal:  J Bacteriol       Date:  2002-01       Impact factor: 3.490

5.  Adhesion of type 1-fimbriated Escherichia coli to abiotic surfaces leads to altered composition of outer membrane proteins.

Authors:  K Otto; J Norbeck; T Larsson; K A Karlsson; M Hermansson
Journal:  J Bacteriol       Date:  2001-04       Impact factor: 3.490

6.  Suppression of phospholipid biosynthesis by cerulenin in the condensed Single-Protein-Production (cSPP) system.

Authors:  Lili Mao; Koichi Inoue; Yisong Tao; Gaetano T Montelione; Ann E McDermott; Masayori Inouye
Journal:  J Biomol NMR       Date:  2011-01-30       Impact factor: 2.835

7.  Ferric uptake regulator and its role in the pathogenesis of nontypeable Haemophilus influenzae.

Authors:  Alistair Harrison; Estevan A Santana; Blake R Szelestey; David E Newsom; Peter White; Kevin M Mason
Journal:  Infect Immun       Date:  2013-02-04       Impact factor: 3.441

8.  Subcellular fractions of Brucella ovis distinctively induce the production of interleukin-2, interleukin-4, and interferon-gamma in mice.

Authors:  E Salas-Téllez; A Núñez del Arco; V Tenorio; E Díaz-Aparicio; M de la Garza; F Suárez-Güemes
Journal:  Can J Vet Res       Date:  2005-01       Impact factor: 1.310

Review 9.  Diagnosis and assessment of trachoma.

Authors:  Anthony W Solomon; Rosanna W Peeling; Allen Foster; David C W Mabey
Journal:  Clin Microbiol Rev       Date:  2004-10       Impact factor: 26.132

10.  Cloning and expression of the pilin gene of Pseudomonas aeruginosa PAK in Escherichia coli.

Authors:  M S Strom; S Lory
Journal:  J Bacteriol       Date:  1986-02       Impact factor: 3.490
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