Literature DB >> 4958775

Isotopic method for estimating microbial cell volumes.

D F Reid, H A Frank.   

Abstract

Reid, Della F. (University of Hawaii, Honolulu), and Hilmer A. Frank. Isotopic method for estimating microbial cell volumes. J. Bacteriol. 92:639-644. 1966.-A sensitive isotopic method was developed for estimating the mean cell volume in small numbers of bacterial spores by measuring directly the extracellular fluid volume when cells are present in a saturated inulin solution containing inulin-C(14) at low specific activity. The method minimizes errors due to solute adsorption to the cells and avoids errors arising from differences in cell packing. The effects of these sources of error on cell volume and intercellular space estimations made with nonpenetrating solutes are discussed.

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Year:  1966        PMID： 4958775      PMCID： PMC276303          DOI： 10.1128/jb.92.3.639-644.1966

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  16 in total

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6.  Bacterial permeability: the uptake and oxidation of citrate by Escherichia coli.

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  5 in total

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