Literature DB >> 4942759

Biochemical characterization of the ctr mutants of Escherichia coli.

H G Morse, W K Penberthy, M L Morse.   

Abstract

A test procedure based on complementation in mixed extracts is described for the assay of heat-stable protein and enzyme I of the phosphoenolpyruvate-dependent phosphotransferase system. The test was used to assay a collection of pleiotropic carbohydrate mutants of Escherichia coli (ctr mutants) and revertants of these mutants. All mutants were found to lack enzyme I of the phosphoenolpyruvate-dependent transferase system. Revertants of these mutants to complete wild phenotype regained enzyme I-forming ability. Reversion to partial wild type was not accompanied by restoration of enzyme I-forming ability.

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Year:  1971        PMID： 4942759      PMCID： PMC247127          DOI： 10.1128/jb.108.2.690-694.1971

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  10 in total

1.  An agar medium indicating acid production.

Authors:  M L MORSE; M L ALIRE
Journal:  J Bacteriol       Date:  1958-09       Impact factor: 3.490

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

Review 3.  Current linkage map of Escherichia coli.

Authors:  A L Taylor
Journal:  Bacteriol Rev       Date:  1970-06

4.  The physiological behavior of enzyme I and heat-stable protein mutants of a bacterial phosphotransferase system.

Authors:  M H Saier; R D Simoni; S Roseman
Journal:  J Biol Chem       Date:  1970-11-10       Impact factor: 5.157

5.  The role of a phosphoenolpyruvate-dependent kinase system in beta-glucoside catabolism in Escherichia coli.

Authors:  C F Fox; G Wilson
Journal:  Proc Natl Acad Sci U S A       Date:  1968-03       Impact factor: 11.205

6.  The enzymatic lesion of strain MM-6, a pleiotropic carbohydrate-negative mutant of Escherichia coli.

Authors:  S Tanaka; D G Fraenkel; E C Lin
Journal:  Biochem Biophys Res Commun       Date:  1967-04-07       Impact factor: 3.575

7.  Carbohydrate accumulation and metabolism in Escherichia coli. I. Description of pleiotropic mutants.

Authors:  R J Wang; M L Morse
Journal:  J Mol Biol       Date:  1968-02-28       Impact factor: 5.469

8.  Carbohydrate accumulation and metabolism in Escherichia coli: the close linkage and chromosomal location of ctr mutations.

Authors:  R J Wang; H G Morse; M L Morse
Journal:  J Bacteriol       Date:  1969-05       Impact factor: 3.490

9.  Effect of pleiotropic carbohydrate mutations (ctr) on tryptophan catabolism.

Authors:  R Dahl; R J Wang; M L Morse
Journal:  J Bacteriol       Date:  1971-08       Impact factor: 3.490

10.  Carbohydrate Accumulation and Metabolism in Escherichia coli: Characteristics of the Reversions of ctr Mutations.

Authors:  R J Wang; H G Morse; M L Morse
Journal:  J Bacteriol       Date:  1970-12       Impact factor: 3.490
  10 in total
  5 in total

1.  Genetic analyses of an amber mutation in Escherichia coli K-12, affecting deoxyribonucleic acid ligase and viability.

Authors:  T Sato; T Horiuchi; T Nagata
Journal:  J Bacteriol       Date:  1975-12       Impact factor: 3.490

2.  Carbohydrate transport and cyclic 3',5' adenosine monophosphate (cAMP) levels in a temperature sensitive phosphotransferase mutant of Escherichia coli.

Authors:  R Dahl; H G Morse; M L Morse
Journal:  Mol Gen Genet       Date:  1974-03-06

Review 3.  Phosphoenolpyruvate:carbohydrate phosphotransferase system of bacteria.

Authors:  P W Postma; J W Lengeler
Journal:  Microbiol Rev       Date:  1985-09

4.  Catabolite repression in Escherichia coli K12 mutants defective in glucose transport.

Authors:  V N Gershanovitch; N V Yourovitskaya; L V Komissarova; T N Bolshakova; R S Erlagaeva; G I Bourd
Journal:  Mol Gen Genet       Date:  1975-09-15

5.  A second purine nucleoside phosphorylase in Escherichia coli K-12. I. Xanthosine phosphorylase regulatory mutants isolated as secondary-site revertants of a deoD mutant.

Authors:  R S Buxton; K Hammer-Jespersen; P Valentin-Hansen
Journal:  Mol Gen Genet       Date:  1980
  5 in total

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