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Literature DB >> 1104569

Genetic analyses of an amber mutation in Escherichia coli K-12, affecting deoxyribonucleic acid ligase and viability.

Abstract

Genetic analyses of an Escherichia coli K-12 mutant possessing the amber mutation lig-321 were carried out. This mutant is defective in deoxyribonucleic acid (DNA) ligase and conditionally lethal. We constructed strains harboring an F'lig+ or F'lig-321 plasmid. Genetic complementation analyses were done by using these plasmids and by constructing a lig-4/F'lig-321 merodiploid. It was shown that lig-321 does not complement lig-4, unless the former is suppressed by an amber suppressor. The same was found to be the case between lig-321 and lig-ts7. Transductional mapping of lig-321, by a four-factor cross, revealed that lig-321 is very closely linked to lig-4. The frequency of recombinants between the two alleles was not unreasonable for assuming that they arose by intragenic recombination. The lig-4 and lig-ts7 alleles are known to reside in the structural gene for DNA ligase, in which lig-321 may also be located.

Entities: Gene Species

Mesh：

Substances：
Polynucleotide Ligases

Year: 1975 PMID： 1104569 PMCID： PMC236010 DOI： 10.1128/jb.124.3.1089-1096.1975

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

24 in total

1. DNA degradation in an amber mutant of Escherichia coli K12 affecting DNA ligase and viability.

Authors: T Horiuchi; T Sato; T Nagata
Journal: J Mol Biol Date: 1975-06-25 Impact factor: 5.469

2. Lethality of the Escherichia coli K12 cell doubly deficient in DNA polymerase I and DNA strand-joining activity.

Authors: T Horiuchi; T Nagata
Journal: Mol Gen Genet Date: 1974

3. Distribution of closely linked markers following intragenic recombination in Escherichia coli.

Authors: I P Crawford; J Preiss
Journal: J Mol Biol Date: 1972-11-28 Impact factor: 5.469

4. Isolation and characterization of a temperature-sensitive amber suppressor mutant of Escherichia coli K12.

Authors: T Nagata; T Horiuchi
Journal: Mol Gen Genet Date: 1973

5. Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I.

Authors: M Monk; J Kinross
Journal: J Bacteriol Date: 1972-03 Impact factor: 3.490

6. Genetic and enzymatic characterization of a conditional lethal mutant of Escherichia coli K12 with a temperature-sensitive DNA ligase.

Authors: E B Konrad; P Modrich; I R Lehman
Journal: J Mol Biol Date: 1973-07-15 Impact factor: 5.469

2. Regulatory mutations conferring constitutive synthesis of major outer membrane proteins (OmpC and OmpF) in Escherichia coli.

Authors: T Sato; T Yura
Journal: J Bacteriol Date: 1981-01 Impact factor: 3.490

Review 3. Linkage map of Escherichia coli K-12, edition 6.

Authors: B J Bachmann; K B Low
Journal: Microbiol Rev Date: 1980-03

4. Chromosomal location and expression of the structural gene for major outer membrane protein Ia of Escherichia coli K-12 and of the homologous gene of Salmonella typhimurium.

Authors: T Sato; T Yura
Journal: J Bacteriol Date: 1979-08 Impact factor: 3.490

5. Escherichia coli mutants incapable of supporting replication of F-like plasmids at high temperature: isolation and characterization of mafA and mafB mutants.

Authors: C Wada; T Yura
Journal: J Bacteriol Date: 1979-12 Impact factor: 3.490

5 in total

Genetic analyses of an amber mutation in Escherichia coli K-12, affecting deoxyribonucleic acid ligase and viability.

1. DNA degradation in an amber mutant of Escherichia coli K12 affecting DNA ligase and viability.

2. Lethality of the Escherichia coli K12 cell doubly deficient in DNA polymerase I and DNA strand-joining activity.

3. Distribution of closely linked markers following intragenic recombination in Escherichia coli.

4. Isolation and characterization of a temperature-sensitive amber suppressor mutant of Escherichia coli K12.

5. Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I.

6. Genetic and enzymatic characterization of a conditional lethal mutant of Escherichia coli K12 with a temperature-sensitive DNA ligase.

7. Mutations affecting growth of the Escherichia coli cell under a condition of DNA polymerase I-deficiency.

8. Genetics and function of DNA ligase in Escherichia coli.

9. Polarity and enzyme functions in mutants of the first three genes of the tryptophan operon of Escherichia coli.

10. Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12.

1. Genetic studies of an Escherichia coli K-12 temperature-sensitive mutant defective in membrane protein synthesis.

2. Regulatory mutations conferring constitutive synthesis of major outer membrane proteins (OmpC and OmpF) in Escherichia coli.

Review 3. Linkage map of Escherichia coli K-12, edition 6.

4. Chromosomal location and expression of the structural gene for major outer membrane protein Ia of Escherichia coli K-12 and of the homologous gene of Salmonella typhimurium.

5. Escherichia coli mutants incapable of supporting replication of F-like plasmids at high temperature: isolation and characterization of mafA and mafB mutants.