Literature DB >> 493130

Detection of labelled RNA species by contact hybridization.

J Burckhardt, J Telford, M L Birnstiel.   

Abstract

An improved contact hybridization technique for the analysis of labelled RNA species is presented. The method combines high sensitivity of detection with the high resolution of polyacrylamide gel electrophoresis and should be especially useful for the characterization of transient RNA precursor molecules. Its application to gene mapping is illustrated.

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Year:  1979        PMID： 493130      PMCID： PMC327910          DOI： 10.1093/nar/6.9.2963

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  12 in total

1.  Integration of eukaryotic genes for 5S RNA and histone proteins into a phage lambda receptor.

Authors:  S G Clarkson; H O Smith; W Schaffner; K W Gross; M L Birnstiel
Journal:  Nucleic Acids Res       Date:  1976-10       Impact factor: 16.971

2.  Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.

Authors:  R A Laskey; A D Mills
Journal:  FEBS Lett       Date:  1977-10-15       Impact factor: 4.124

3.  A thermostable sequence-specific endonuclease from Thermus aquaticus.

Authors:  S Sato; C A Hutchinson; J I Harris
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

4.  Analysis of histone messenger RNA of Drosophila melanogaster by two-dimensional gel electrophoresis.

Authors:  J Burckhardt; M L Birnstiel
Journal:  J Mol Biol       Date:  1978-01-05       Impact factor: 5.469

5.  Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.

Authors:  J C Alwine; D J Kemp; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

6.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors:  W M Bonner; R A Laskey
Journal:  Eur J Biochem       Date:  1974-07-01

7.  Genes and spacers of cloned sea urchin histone DNA analyzed by sequencing.

Authors:  W Schaffner; G Kunz; H Daetwyler; J Telford; H O Smith; M L Birnstiel
Journal:  Cell       Date:  1978-07       Impact factor: 41.582

8.  Mapping of the Xenopus laevis 5.8S rDNA by restriction and DNA sequencing.

Authors:  P G Boseley; A Tuyns; M L Birnstiel
Journal:  Nucleic Acids Res       Date:  1978-04       Impact factor: 16.971

9.  Molecular analysis of the histone gene cluster of Psammechinus miliaris: I. Fractionation and identification of five individual histone mRNAs.

Authors:  K Gross; E Probst; W Schaffner; M Birnstiel
Journal:  Cell       Date:  1976-08       Impact factor: 41.582

10.  Molecular analysis of the histone gene cluster of psammechinus miliaris: II. The arrangement of the five histone-coding and spacer sequences.

Authors:  W Schaffner; K Gross; J Telford; M Birnstiel
Journal:  Cell       Date:  1976-08       Impact factor: 41.582
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  13 in total

1.  Topography of the protein complexes of the chloroplast thylakoid membrane : studies of photosystem I using a chemical probe and proteolytic digestion.

Authors:  W Ortiz; E Lam; S Chollar; D Munt; R Malkin
Journal:  Plant Physiol       Date:  1985-02       Impact factor: 8.340

2.  Differential tissue expression of the glutathione transferase multigene family.

Authors:  S E Pemble; J B Taylor; R K Craig; B Ketterer
Journal:  Biochem J       Date:  1986-09-01       Impact factor: 3.857

3.  Characterization of translation products of the polyadenylated RNA of free and membrane-bound polyribosomes of rat forebrain.

Authors:  C Hall; L Mahadevan; S Whatley; G Biswas; L Lim
Journal:  Biochem J       Date:  1984-05-01       Impact factor: 3.857

4.  Alterations in the relative amounts of specific mRNA species in the developing human brain in Down's syndrome.

Authors:  S A Whatley; C Hall; A N Davison; L Lim
Journal:  Biochem J       Date:  1984-05-15       Impact factor: 3.857

5.  Construction and characterization of a plasmid containing complementary DNA to mRNA encoding the N-terminal amino acid sequence of the rat glutathione transferase Ya subunit.

Authors:  J B Taylor; R K Craig; D Beale; B Ketterer
Journal:  Biochem J       Date:  1984-04-01       Impact factor: 3.857

6.  The construction and partial characterization of plasmids containing complementary DNA sequences to human calcitonin precursor polyprotein.

Authors:  J Allison; L Hall; I MacIntyre; R K Craig
Journal:  Biochem J       Date:  1981-12-01       Impact factor: 3.857

7.  Identification and subsequent phosphorylation of sequestered partially processed caseins in the lactating guinea-pig mammary gland.

Authors:  A P Boulton; J C Pascall; R K Craig
Journal:  Biochem J       Date:  1984-09-01       Impact factor: 3.857

8.  The construction, identification and partial characterization of plasmids containing guinea-pig milk protein complementary DNA sequences.

Authors:  R K Craig; L Hall; D Parker; P N Campbell
Journal:  Biochem J       Date:  1981-03-15       Impact factor: 3.857

9.  Restriction endonuclease mapping by crossed contact hybridization: the ribosomal RNA genes of Achlya ambisexualis.

Authors:  C E Rozek; W E Timberlake
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

10.  Detection of vesicular stomatitis virus RNA and its defective-interfering particles in individual mouse brains.

Authors:  D R Cave; F S Hagen; E L Palma; A S Huang
Journal:  J Virol       Date:  1984-04       Impact factor: 5.103
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