| Literature DB >> 7306038 |
R K Craig, L Hall, D Parker, P N Campbell.
Abstract
A complementary DNA (cDNA) plasmid library has been constructed in the plasmid pAT153, using poly(A)-containing RNA isolated from the lactating guinea-pig mammary gland as the starting material. Double stranded cDNA was inserted into the EcoRI site of the plasmid using poly(dA . dT) tails, then transformed into Escherichia coli HB101. From the resulting colonies we have selected and partially characterized plasmids containing cDNA copies of the mRNAs for casein A, casein B, casein C and alpha-lactalbumin. However, the proportion containing casein C cDNA was exceptionally low, and these contained at best 60% of the mRNA sequence.Entities:
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Year: 1981 PMID: 7306038 PMCID: PMC1162837 DOI: 10.1042/bj1940989
Source DB: PubMed Journal: Biochem J ISSN: 0264-6021 Impact factor: 3.857