Literature DB >> 493115

Translation of specific vaccinia virus RNAs purified as RNA-DNA hybrids on potassium iodide gradients.

J A Cooper, B Moss.   

Abstract

A procedure has been developed for purifying specific mRNAs by hybridization to fragments of DNA and isolation of the hybrids by potassium iodide equilibrium buoyant density centrifugation. The hybrids obtained are essentially free of unhybridized RNA as well as double-stranded RNA. Moreover, the RNA in the hybrids is undamaged and can be translated in vitro. Application of this procedure to mapping vaccinia virus genes is described. A total of 34 polypeptides have been assigned to three regions of the viral genome.

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Year:  1979        PMID： 493115      PMCID： PMC327959          DOI： 10.1093/nar/6.11.3599

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  25 in total

1.  Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.

Authors:  J Casey; N Davidson
Journal:  Nucleic Acids Res       Date:  1977       Impact factor: 16.971

2.  Inverted terminal repeats in rabbit poxvirus and vaccinia virus DNA.

Authors:  R Wittek; A Menna; H K Müller; D Schümperli; P G Boseley; R Wyler
Journal:  J Virol       Date:  1978-10       Impact factor: 5.103

3.  Transcriptional complexity of vaccinia virus in vivo and in vitro.

Authors:  E Paoletti; L J Grady
Journal:  J Virol       Date:  1977-09       Impact factor: 5.103

4.  Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.

Authors:  B M Paterson; B E Roberts; E L Kuff
Journal:  Proc Natl Acad Sci U S A       Date:  1977-10       Impact factor: 11.205

5.  Visualization of an inverted terminal repetition in vaccinia virus DNA.

Authors:  C F Garon; E Barbosa; B Moss
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

6.  Preparative and analytical purification of DNA from agarose.

Authors:  B Vogelstein; D Gillespie
Journal:  Proc Natl Acad Sci U S A       Date:  1979-02       Impact factor: 11.205

7.  An efficient mRNA-dependent translation system from reticulocyte lysates.

Authors:  H R Pelham; R J Jackson
Journal:  Eur J Biochem       Date:  1976-08-01

8.  Sequence complexity and relative abundance of vaccinia virus mRNA's synthesized in vivo and in vitro.

Authors:  R F Boone; B Moss
Journal:  J Virol       Date:  1978-06       Impact factor: 5.103

9.  Transcription of vaccinia virus mRNA coupled to translation in vitro.

Authors:  J A Cooper; B Moss
Journal:  Virology       Date:  1978-07-01       Impact factor: 3.616

10.  Intermolecular duplexes formed from polyadenylylated vaccinia virus RNA.

Authors:  R F Boone; R P Parr; B Moss
Journal:  J Virol       Date:  1979-04       Impact factor: 5.103
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  9 in total

1.  Endogenous LINE-1 (Long Interspersed Nuclear Element-1) Reverse Transcriptase Activity in Platelets Controls Translational Events Through RNA-DNA Hybrids.

Authors:  Hansjörg Schwertz; Jesse W Rowley; Gerald G Schumann; Ulrike Thorack; Robert A Campbell; Bhanu Kanth Manne; Guy A Zimmerman; Andrew S Weyrich; Matthew T Rondina
Journal:  Arterioscler Thromb Vasc Biol       Date:  2018-01-04       Impact factor: 8.311

2.  Transcriptional and translational analysis of the vaccinia virus late gene L65.

Authors:  S L Weinrich; E G Niles; D E Hruby
Journal:  J Virol       Date:  1985-08       Impact factor: 5.103

3.  A tandemly-oriented late gene cluster within the vaccinia virus genome.

Authors:  S L Weinrich; D E Hruby
Journal:  Nucleic Acids Res       Date:  1986-04-11       Impact factor: 16.971

4.  Noncoordinate regulation of a vaccinia virus late gene cluster.

Authors:  S L Weinrich; D E Hruby
Journal:  J Virol       Date:  1987-03       Impact factor: 5.103

5.  Identification and mapping of two polypeptides encoded within the herpes simplex virus type 1 thymidine kinase gene sequences.

Authors:  C M Preston; D J McGeoch
Journal:  J Virol       Date:  1981-05       Impact factor: 5.103

6.  Marker rescue of temperature-sensitive mutations of vaccinia virus WR: correlation of genetic and physical maps.

Authors:  M J Ensinger; M Rovinsky
Journal:  J Virol       Date:  1983-11       Impact factor: 5.103

7.  Hybridization selection and cell-free translation of mRNA's encoded within the inverted terminal repetition of the vaccinia virus genome.

Authors:  J A Cooper; R Wittek; B Moss
Journal:  J Virol       Date:  1981-01       Impact factor: 5.103

8.  Mapping of the vaccinia virus thymidine kinase gene by marker rescue and by cell-free translation of selected mRNA.

Authors:  J P Weir; G Bajszár; B Moss
Journal:  Proc Natl Acad Sci U S A       Date:  1982-02       Impact factor: 11.205

Review 9.  Organization and expression of the poxvirus genome.

Authors:  R Wittek
Journal:  Experientia       Date:  1982-03-15
  9 in total

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