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Literature DB >> 13917754

Evaluation of extracellular deoxyribonuclease activity in Pseudomonas.

M M STREITFELD, E M HOFFMANN, H M JANKLOW.

Abstract

Streitfeld, Murray M. (University of Miami, Coral Gables, Fla.), Edward M. Hoffmann, and Herbert M. Janklow. Evaluation of extracellular deoxyribonuclease activity in Pseudomonas. J. Bacteriol. 84:77-80. 1962.-Of 23 strains of P. aeruginosa isolated from pathological processes, 22 strains, representative of at least nine different somatic antigen types, were found to elaborate extracellular deoxyribonuclease. Qualitative determinations of enzyme elaboration were performed after the development of a convenient toluidine-blue assay plate procedure, dependent upon the metachromatic properties of the dye. This new procedure permits the determination of enzyme activity despite the presence, in the deoxyribonucleic acid-containing agar medium, of 2 to 5 ml/100 ml serum or plasma, or extracellular pyocyanin.

Entities: Chemical Species

Keywords: DESOXYRIBONUCLEASE/metabolism; PSEUDOMONAS/metabolism

Mesh：

Substances：

Year: 1962 PMID： 13917754 PMCID： PMC277769 DOI： 10.1128/jb.84.1.77-80.1962

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

10 in total

1. [Microbial enzymes; rate of production of gelatinolytic and fibrinolytic diastases of various proteolytic bacteria].

Authors: R KOURILSKY; R RICHOU
Journal: C R Hebd Seances Acad Sci Date: 1954-12-08

2. New medium for the detection of esterase activity of microorganisms.

Authors: M K MUFTIC
Journal: Enzymologia Date: 1954-11-15

3. Lecithinase production by gramnegative bacteria.

Authors: M T ESSELMANN; P V LIU
Journal: J Bacteriol Date: 1961-06 Impact factor: 3.490

4. Qualitative estimation of staphylococcal deoxyribonuclease.

Authors: M H FUSILLO; D L WEISS
Journal: J Bacteriol Date: 1959-10 Impact factor: 3.490

5. Deoxyribonuclease activity of micrococci from clinical sources.

Authors: B G WECKMAN; B W CATLIN
Journal: J Bacteriol Date: 1957-06 Impact factor: 3.490

6. Rapid method for determining the activity of microorganisms on nucleic acids.

Authors: C D JEFFRIES; D F HOLTMAN; D G GUSE
Journal: J Bacteriol Date: 1957-04 Impact factor: 3.490

7. Desoxyribonuclease and coagulase activity of micrococci.

Authors: J W DISALVO
Journal: Med Techn Bull Date: 1958 Sep-Oct

8. Extracellular deoxyribonucleic acid of bacteria and a deoxyribonuclease inhibitor.

Authors: B W CATLIN
Journal: Science Date: 1956-09-07 Impact factor: 47.728

9. Immunity and antibody to Pseudomonas pyocyanea in rabbits.

Authors: J E FOX; E J LOWBURY
Journal: J Pathol Bacteriol Date: 1953-04

10. [Mucolytic activity of certain microorganisms].

Authors: L BERGAMINI
Journal: Boll Ist Sieroter Milan Date: 1952 Sep-Oct

10 in total

11 in total

6. Isolation and characterization of plasmid-deletion derivatives from an Hfr made with Rts1 plasmid carrying a chromosomal mutation, plt.

Authors: C Sasakawa; T Tsuchiya; M Yoshikawa
Journal: Mol Gen Genet Date: 1980-01

Evaluation of extracellular deoxyribonuclease activity in Pseudomonas.

1. [Microbial enzymes; rate of production of gelatinolytic and fibrinolytic diastases of various proteolytic bacteria].

2. New medium for the detection of esterase activity of microorganisms.

3. Lecithinase production by gramnegative bacteria.

4. Qualitative estimation of staphylococcal deoxyribonuclease.

5. Deoxyribonuclease activity of micrococci from clinical sources.

6. Rapid method for determining the activity of microorganisms on nucleic acids.

7. Desoxyribonuclease and coagulase activity of micrococci.

8. Extracellular deoxyribonucleic acid of bacteria and a deoxyribonuclease inhibitor.

9. Immunity and antibody to Pseudomonas pyocyanea in rabbits.

10. [Mucolytic activity of certain microorganisms].

1. Four hour-test for the identification of Enterobacteriaceae.

2. Indicator plates for rapid detection of ribonuclease T1 secreting Escherichia coli clones.

3. Improved toluidine blue-DNA agar for detection of DNA hydrolysis by campylobacters.

4. Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity.

5. Fluorescent method for the detection of excreted ribonuclease around bacterial colonies.

6. Isolation and characterization of plasmid-deletion derivatives from an Hfr made with Rts1 plasmid carrying a chromosomal mutation, plt.

7. Nucleases of some udder pathogenic organisms. In vivo and in vitro production.

8. Improvement of two toluidine blue O-mediated techniques for DNase detection.

9. PARTIAL PURIFICATION OF THE EXTRACELLULAR HEMOLYSIN OF PSEUDOMONAS AERUGINOSA.

10. Use of a plating method to estimate extracellular protein production by staphylococci.