Literature DB >> 488105

A comparison of the initiation factors of eukaryotic protein synthesis from ribosomes and from the postribosomal supernatant.

A Thomas, H Goumans, H Amesz, R Benne, H O Voorma.   

Abstract

A purification procedure is described for the initiation factors of protein synthesis from rabbit reticulocytes: (a) from the ribosomal wash and (b) from the postribosomal supernantant. A comparison is made between these preparations with respect to yield and specific activity. eIF-4A and eIF-4D occur mainly in the postribosomal supernatant; eIF-2, eIF-4C and eIF-5 are more evenly divided over both fractions, whereas eIF-1, eIF-3 and eIF-4B are found almost exclusively in the ribosomal wash. No significant difference in specific activity could be detected when factors from both sources were compared, with a possible exception of eIF-4A and eIF-4D.

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Year:  1979        PMID： 488105     DOI： 10.1111/j.1432-1033.1979.tb13192.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  15 in total

1.  Localization of cap-binding protein in subcellular fractions of HeLa cells.

Authors:  J L Hansen; D O Etchison; J W Hershey; E Ehrenfeld
Journal:  Mol Cell Biol       Date:  1982-12       Impact factor: 4.272

2.  Cowpea mosaic virus middle component RNA contains a sequence that allows internal binding of ribosomes and that requires eukaryotic initiation factor 4F for optimal translation.

Authors:  A A Thomas; E ter Haar; J Wellink; H O Voorma
Journal:  J Virol       Date:  1991-06       Impact factor: 5.103

Review 3.  The hypusine-containing translation factor eIF5A.

Authors:  Thomas E Dever; Erik Gutierrez; Byung-Sik Shin
Journal:  Crit Rev Biochem Mol Biol       Date:  2014-07-17       Impact factor: 8.250

4.  Phosphoprotein B-50: localization of proteolytic sites for S. aureus V8 protease using truncated cRNAs for cell-free translation.

Authors:  H B Nielander; A J Van Rozen; L H Schrama; M Kasparaitis; A B Oestreicher; W H Gispen; P Schotman
Journal:  J Mol Neurosci       Date:  1991       Impact factor: 3.444

5.  Interaction of eukaryotic initiation factor eIF-4B with a picornavirus internal translation initiation site.

Authors:  K Meyer; A Petersen; M Niepmann; E Beck
Journal:  J Virol       Date:  1995-05       Impact factor: 5.103

6.  Association of initiation factor eIF-2 with a rapidly sedimenting fraction from Ehrlich ascites-tumour cells.

Authors:  M J Clemens; V M Pain
Journal:  Biochem J       Date:  1981-01-15       Impact factor: 3.857

7.  Translation initiation factor eIF4B interacts with a picornavirus internal ribosome entry site in both 48S and 80S initiation complexes independently of initiator AUG location.

Authors:  K Ochs; R C Rust; M Niepmann
Journal:  J Virol       Date:  1999-09       Impact factor: 5.103

8.  Effects of translation initiation factor eIF-5A on the functioning of human T-cell leukemia virus type I Rex and human immunodeficiency virus Rev inhibited trans dominantly by a Rex mutant deficient in RNA binding.

Authors:  J Katahira; T Ishizaki; H Sakai; A Adachi; K Yamamoto; H Shida
Journal:  J Virol       Date:  1995-05       Impact factor: 5.103

9.  Shutoff of neuroblastoma cell protein synthesis by Semliki Forest virus: loss of ability of crude initiation factors to recognize early Semliki Forest virus and host mRNA's.

Authors:  H van Steeg; A Thomas; S Verbeek; M Kasperaitis; H O Voorma; R Benne
Journal:  J Virol       Date:  1981-05       Impact factor: 5.103

10.  Eukaryotic initiation factor 5A plays an essential role in luteinizing hormone receptor regulation.

Authors:  Bindu Menon; Thippeswamy Gulappa; K M J Menon
Journal:  Mol Endocrinol       Date:  2014-09-12
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