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Literature DB >> 7197921

Association of initiation factor eIF-2 with a rapidly sedimenting fraction from Ehrlich ascites-tumour cells.

Abstract

To determine the subcellular distribution of initiation-factor eIF-2 activity, Ehrlich ascites-cell homogenates were fractionated to give (a) a rapidly sedimenting fraction, (b) a microsomal fraction and (c) post-microsomal supernatant. The first two fractions were washed in 0.5 m-KCl to render the associated protein-synthesis factor soluble. As much as 60% of the total recoverable eIF-2 was obtained from the rapidly sedimenting material.

Entities: Chemical Disease Gene

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Year: 1981 PMID： 7197921 PMCID： PMC1162751 DOI： 10.1042/bj1940357

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

25 in total

1. Interferon, double-stranded RNA, and protein phosphorylation.

Authors: B Lebleu; G C Sen; S Shaila; B Cabrer; P Lengyel
Journal: Proc Natl Acad Sci U S A Date: 1976-09 Impact factor: 11.205

2. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

3. Starvation results in decreased initiation factor activity in rat skeletal muscle.

Authors: D E Rannels; A E Pegg; S R Rannels
Journal: Biochem Biophys Res Commun Date: 1976-10-18 Impact factor: 3.575

4. Purification and properties of a methionine formylmethionyl-tRNA-binding initiation factor from pig liver.

Authors: I Harbitz; J G Hauge
Journal: Arch Biochem Biophys Date: 1976-10 Impact factor: 4.013

5. Protein synthesis initiation in eukaryotes. Characterization of ribosomal factors from mouse fibroblasts.

Authors: D H Levin; D Kyner; G Acs
Journal: J Biol Chem Date: 1973-09-25 Impact factor: 5.157

6. Met-tRNAfMet binding to 40S ribosomal subunits: a site for the regulation of initiation of protein synthesis by hemin.

Authors: M J Clemens; E C Henshaw; H Rahamimoff; I M London
Journal: Proc Natl Acad Sci U S A Date: 1974-08 Impact factor: 11.205

7. A cytoskeletal structure with associated polyribosomes obtained from HeLa cells.

Authors: R Lenk; L Ransom; Y Kaufmann; S Penman
Journal: Cell Date: 1977-01 Impact factor: 41.582

8. Initiation of protein synthesis in Ehrlich ascites tumour cells. Evidence for physiological variation in the association of methionyl-tRNAf with native 40-S ribosomal subunits in vivo.

Authors: V M Pain; E C Henshaw
Journal: Eur J Biochem Date: 1975-09-15

Association of initiation factor eIF-2 with a rapidly sedimenting fraction from Ehrlich ascites-tumour cells.

1. Interferon, double-stranded RNA, and protein phosphorylation.

2. Protein measurement with the Folin phenol reagent.

3. Starvation results in decreased initiation factor activity in rat skeletal muscle.

4. Purification and properties of a methionine formylmethionyl-tRNA-binding initiation factor from pig liver.

5. Protein synthesis initiation in eukaryotes. Characterization of ribosomal factors from mouse fibroblasts.

6. Met-tRNAfMet binding to 40S ribosomal subunits: a site for the regulation of initiation of protein synthesis by hemin.

7. A cytoskeletal structure with associated polyribosomes obtained from HeLa cells.

8. Initiation of protein synthesis in Ehrlich ascites tumour cells. Evidence for physiological variation in the association of methionyl-tRNAf with native 40-S ribosomal subunits in vivo.

9. Studies on free and membrane-bound ribosomes in rat liver. I. Distribution as related to total cellular RNA.

10. A rapidly sedimenting fraction of rat liver endoplasmic reticulum.

1. Effect of alanine supply on hepatic protein synthesis in animals maintained on a protein free diet.

2. Effect of vasopressin on the regulation of protein synthesis initiation in liver cells.