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Literature DB >> 14582205

Localization of cap-binding protein in subcellular fractions of HeLa cells.

J L Hansen¹, D O Etchison, J W Hershey, E Ehrenfeld.

Abstract

The 26,000-M(r) cap-binding protein was analyzed by a cross-linking assay in cell fractions from uninfected and poliovirus-infected HeLa cells. Cap-binding protein was found in the postribosomal supernatant (S-200) and in the ribosomal salt wash. The cap-binding protein in the S-200 had a sedimentation coefficient of 5 to 7S and lacked the ability to restore translation in extracts of poliovirus-infected cells.

Entities: Chemical Disease Species

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Year: 1982 PMID： 14582205 PMCID： PMC369972 DOI： 10.1128/mcb.2.12.1639-1643.1982

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

14 in total

1. Inhibition of translation by poliovirus: inactivation of a specific initiation factor.

Authors: J K Rose; H Trachsel; K Leong; D Baltimore
Journal: Proc Natl Acad Sci U S A Date: 1978-06 Impact factor: 11.205

2. Differential stimulation of capped mRNA translation in vitro by cap binding protein.

Authors: N Sonenberg; H Trachsel; S Hecht; A J Shatkin
Journal: Nature Date: 1980-05-29 Impact factor: 49.962

3. Control of protein synthesis in extracts from poliovirus-infected cells. I. mRNA discrimination by crude initiation factors.

Authors: T Helentjaris; E Ehrenfeld
Journal: J Virol Date: 1978-05 Impact factor: 5.103

4. Eukaryotic mRNA cap binding protein: purification by affinity chromatography on sepharose-coupled m7GDP.

Authors: N Sonenberg; K M Rupprecht; S M Hecht; A J Shatkin
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

5. In vitro translation in reovirus- and poliovirus-infected cell extracts. Effects of anti-cap binding protein monoclonal antibody.

Authors: N Sonenberg; D Skup; H Trachsel; S Millward
Journal: J Biol Chem Date: 1981-05-10 Impact factor: 5.157

6. Purification of a factor that restores translation of vesicular stomatitis virus mRNA in extracts from poliovirus-infected HeLa cells.

Authors: H Trachsel; N Sonenberg; A J Shatkin; J K Rose; K Leong; J E Bergmann; J Gordon; D Baltimore
Journal: Proc Natl Acad Sci U S A Date: 1980-02 Impact factor: 11.205

7. ATP/Mg++-dependent cross-linking of cap binding proteins to the 5' end of eukaryotic mRNA.

Authors: N Sonenberg
Journal: Nucleic Acids Res Date: 1981-04-10 Impact factor: 16.971

8. A comparison of the initiation factors of eukaryotic protein synthesis from ribosomes and from the postribosomal supernatant.

Authors: A Thomas; H Goumans; H Amesz; R Benne; H O Voorma
Journal: Eur J Biochem Date: 1979-08-01

4. Dominant negative mutants of mammalian translation initiation factor eIF-4A define a critical role for eIF-4F in cap-dependent and cap-independent initiation of translation.

Authors: A Pause; N Méthot; Y Svitkin; W C Merrick; N Sonenberg
Journal: EMBO J Date: 1994-03-01 Impact factor: 11.598

4 in total

Localization of cap-binding protein in subcellular fractions of HeLa cells.

1. Inhibition of translation by poliovirus: inactivation of a specific initiation factor.

2. Differential stimulation of capped mRNA translation in vitro by cap binding protein.

3. Control of protein synthesis in extracts from poliovirus-infected cells. I. mRNA discrimination by crude initiation factors.

4. Eukaryotic mRNA cap binding protein: purification by affinity chromatography on sepharose-coupled m7GDP.

5. In vitro translation in reovirus- and poliovirus-infected cell extracts. Effects of anti-cap binding protein monoclonal antibody.

6. Purification of a factor that restores translation of vesicular stomatitis virus mRNA in extracts from poliovirus-infected HeLa cells.

7. ATP/Mg++-dependent cross-linking of cap binding proteins to the 5' end of eukaryotic mRNA.

8. A comparison of the initiation factors of eukaryotic protein synthesis from ribosomes and from the postribosomal supernatant.

9. Reovirus mRNA can be covalently crosslinked via the 5' cap to proteins in initiation complexes.

10. Presence of the cap-binding protein in initiation factor preparations from poliovirus-infected HeLa cells.

1. Isolation and structural characterization of cap-binding proteins from poliovirus-infected HeLa cells.

2. CAP binding proteins associated with the nucleus.

3. A fraction of the mRNA 5' cap-binding protein, eukaryotic initiation factor 4E, localizes to the nucleus.

4. Dominant negative mutants of mammalian translation initiation factor eIF-4A define a critical role for eIF-4F in cap-dependent and cap-independent initiation of translation.