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Literature DB >> 4868365

Selection of temperature-sensitive activating enzyme mutants in Escherichia coli.

Abstract

A method based on temperature-conditional resistance to thymineless death has been designed to facilitate the isolation of amino acid-activating enzyme mutants. This method may be modified to obtain a greater or lesser proportion of activating enzyme mutants. In the latter instance, an increased proportion of temperature-sensitive macromolecule mutants of other types is obtained. Additional uses of this procedure are discussed.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 1968 PMID： 4868365 PMCID： PMC252122 DOI： 10.1128/jb.95.3.991-997.1968

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

16 in total

1. Unbalanced growth and bacterial death in thymine-deficient and ultraviolet irradiated Escherichia coli.

Authors: H OKAGAKI; Y TSUBOTA; A SIBATANI
Journal: J Bacteriol Date: 1960-12 Impact factor: 3.490

2. Regulation of ribosomal and transfer RNA synthesis.

Authors: C G KURLAND; O MAALOE
Journal: J Mol Biol Date: 1962-03 Impact factor: 5.469

3. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

4. On the mechanism of thymineless death in Bacillus subtilis.

Authors: R Rolfe
Journal: Proc Natl Acad Sci U S A Date: 1967-01 Impact factor: 11.205

5. Use of thymineless death to enrich for doubly auxotrophic mutants of Bacillus megaterium.

Authors: J T Wachsman; L Hogg
Journal: J Bacteriol Date: 1964-05 Impact factor: 3.490

Review 6. Roles of amino acid activating enzymes in cellular physiology.

Authors: F C Neidhardt
Journal: Bacteriol Rev Date: 1966-12

7. Control of three isoenzymic 7-phospho-2-oxo-3-deoxy-Darabino-heptonate-D-erythrose-4-phosphate lyases of Escherichia coli W and derived mutants by repressive and "inductive" effects of the aromatic amino acids.

Authors: K D Brown; C H Doy
Journal: Biochim Biophys Acta Date: 1966-04-12

8. Polysome transitions and the regulation of ribonucleic acid synthesis in Escherichia coli.

Authors: D W Morris; J A DeMoss
Journal: Proc Natl Acad Sci U S A Date: 1966-07 Impact factor: 11.205

9. Histidine regulatory mutants in Salmonella typhimurium II. Histidine regulatory mutants having altered histidyl-tRNA synthetase.

Authors: J R Roth; B N Ames
Journal: J Mol Biol Date: 1966-12-28 Impact factor: 5.469

Review 10. Regulation of chromosome replication and segregation in bacteria.

Authors: K G Lark
Journal: Bacteriol Rev Date: 1966-03

21 in total

1. Thermosensitive mutants of Escherichia coli K-12 altered in the catalytic Subunit and in a Regulatory factor of the glutamy-transfer ribonucleic acid synthetase.

Authors: J Lapointe; G Delcuve
Journal: J Bacteriol Date: 1975-05 Impact factor: 3.490

10. Regulation of the tyrosine biosynthetic enzymes in Salmonella typhimurium: analysis of the involvement of tyrosyl-transfer ribonucleic acid and tyrosyl-transfer ribonucleic acid synthetase.

Authors: J Heinonen; S W Artz; H Zalkin
Journal: J Bacteriol Date: 1972-12 Impact factor: 3.490

Selection of temperature-sensitive activating enzyme mutants in Escherichia coli.

1. Unbalanced growth and bacterial death in thymine-deficient and ultraviolet irradiated Escherichia coli.

2. Regulation of ribosomal and transfer RNA synthesis.

3. Protein measurement with the Folin phenol reagent.

4. On the mechanism of thymineless death in Bacillus subtilis.

5. Use of thymineless death to enrich for doubly auxotrophic mutants of Bacillus megaterium.

Review 6. Roles of amino acid activating enzymes in cellular physiology.

7. Control of three isoenzymic 7-phospho-2-oxo-3-deoxy-Darabino-heptonate-D-erythrose-4-phosphate lyases of Escherichia coli W and derived mutants by repressive and "inductive" effects of the aromatic amino acids.

8. Polysome transitions and the regulation of ribonucleic acid synthesis in Escherichia coli.

9. Histidine regulatory mutants in Salmonella typhimurium II. Histidine regulatory mutants having altered histidyl-tRNA synthetase.

Review 10. Regulation of chromosome replication and segregation in bacteria.

1. Thermosensitive mutants of Escherichia coli K-12 altered in the catalytic Subunit and in a Regulatory factor of the glutamy-transfer ribonucleic acid synthetase.

2. Temperature-sensitive breakdown in vivo of polysomes with mutants of Chlamydomonas reinhardii.

Review 3. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

4. Temperature-sensitive relaxed Phenotype in a stringent strain of Escherichia coli.

5. In vivo translation of amber and ochre codons in Escherichia coli.

6. Screening procedure for Escherichia coli mutants deficient in ribonuclease I.

7. Chloramphenicol and the stimulation of ribonucleic acid synthesis in Escherichia coli.

8. Unusual valyl-transfer ribonucleic acid synthetase mutant of Escherichia coli.

9. Localization of a portion of the ribosomal RNA genes in Escherichia coli.

10. Regulation of the tyrosine biosynthetic enzymes in Salmonella typhimurium: analysis of the involvement of tyrosyl-transfer ribonucleic acid and tyrosyl-transfer ribonucleic acid synthetase.