Literature DB >> 479112

High-frequency fusion of Streptomyces parvulus or Streptomyces antibioticus protoplasts induced by polyethylene glycol.

K Ochi, M J Hitchcock, E Katz.   

Abstract

Conditions were established for the regeneration of protoplasts of Streptomyces parvulus and Streptomyces antibioticus to the mycelial form. Regeneration was accomplished with a hypertonic medium that contained sucrose, CaCl2, MgCl2, and low levels of phosphate. High-frequency fusion of protoplasts derived from auxotrophic strains of S. parvulus or S. antibioticus was induced by polyethylene glycol 4,000 (42%, wt/vol). The frequency of genetic transfer by the fusogenic procedure varied with the auxotrophic strains examined. Fusion with auxotrophic strains of S. parvulus resulted in the formation of true prototrophic recombinants. Similar studies with S. antibioticus revealed that both stable prototrophic recombinants and heterokaryons were formed.

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Year:  1979        PMID： 479112      PMCID： PMC218046          DOI： 10.1128/jb.139.3.984-992.1979

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  18 in total

1.  Heterozygous clones in Streptomyces coelicolor.

Authors:  D A HOPWOOD; G SERMONTI; I SPADA-SERMONTI
Journal:  J Gen Microbiol       Date:  1963-02

2.  Controlled biosynthesis of actinomycin with sarcosine.

Authors:  E KATZ; W A GOSS
Journal:  Biochem J       Date:  1959-11       Impact factor: 3.857

3.  Genetic studies on microbial cross resistance to toxic agents. I. Cross resistance of Escherichia coli to fifteen antibiotics.

Authors:  W SZYBALSKI; V BRYSON
Journal:  J Bacteriol       Date:  1952-10       Impact factor: 3.490

4.  Protoplast fusion of Schizosaccharomyces pombe Auxotrophic mutants of identical mating-type.

Authors:  M Sipiczki; L Ferenczy
Journal:  Mol Gen Genet       Date:  1977-02-28

5.  Polyethylene glycol-induced fusion of heat-inactivated and living protoplasts of Bacillus megaterium.

Authors:  K Fodor; E Demiri; L Alföldi
Journal:  J Bacteriol       Date:  1978-07       Impact factor: 3.490

6.  Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration.

Authors:  R H Baltz
Journal:  J Gen Microbiol       Date:  1978-07

7.  Fusion of yeast spheroplasts.

Authors:  P van Solingen; J B van der Plaat
Journal:  J Bacteriol       Date:  1977-05       Impact factor: 3.490

8.  TYROSINASE INHERITANCE IN STREPTOMYCES SCABIES. I. GENETIC RECOMBINATION.

Authors:  K F GREGORY; J C HUANG
Journal:  J Bacteriol       Date:  1964-06       Impact factor: 3.490

9.  Bacterial protoplast fusion: recombination in fused protoplasts of Streptomyces coelicolor.

Authors:  D A Hopwood; H M Wright
Journal:  Mol Gen Genet       Date:  1978-07-04

10.  Actinomycin biosynthesis by protoplasts derived from Streptomyces parvulus.

Authors:  M J Hitchcock; E Katz
Journal:  Antimicrob Agents Chemother       Date:  1978-01       Impact factor: 5.191
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  11 in total

1.  Antibiotic overproduction by rpsL and rsmG mutants of various actinomycetes.

Authors:  Yukinori Tanaka; Mamoru Komatsu; Susumu Okamoto; Shinji Tokuyama; Akira Kaji; Haruo Ikeda; Kozo Ochi
Journal:  Appl Environ Microbiol       Date:  2009-05-15       Impact factor: 4.792

2.  Streptomyces relC mutants with an altered ribosomal protein ST-L11 and genetic analysis of a Streptomyces griseus relC mutant.

Authors:  K Ochi
Journal:  J Bacteriol       Date:  1990-07       Impact factor: 3.490

3.  S-Adenosyl-L-methionine: macrocin O-methyltransferase activities in a series of Streptomyces fradiae mutants that produce different levels of the macrolide antibiotic tylosin.

Authors:  E T Seno; R H Baltz
Journal:  Antimicrob Agents Chemother       Date:  1982-05       Impact factor: 5.191

4.  Plasmid transfer and genetic recombination by protoplast fusion in staphylococci.

Authors:  F Götz; S Ahrné; M Lindberg
Journal:  J Bacteriol       Date:  1981-01       Impact factor: 3.490

5.  Occurrence of reiterated DNA sequences in strains of Streptomyces produced by an interspecific protoplast fusion.

Authors:  M Robinson; E Lewis; E Napier
Journal:  Mol Gen Genet       Date:  1981

6.  Genetic recombination in Micromonospora rosaria by protoplast fusion.

Authors:  D D Ryu; K S Kim; N Y Cho; H S Pai
Journal:  Appl Environ Microbiol       Date:  1983-06       Impact factor: 4.792

7.  Protoplast fusion permits high-frequency transfer of a Streptomyces determinant which mediates actinomycin synthesis.

Authors:  K Ochi
Journal:  J Bacteriol       Date:  1982-05       Impact factor: 3.490

8.  Control of the actinomycin biosynthetic pathway in and actinomycin resistance of Streptomyces spp.

Authors:  K Ochi
Journal:  J Bacteriol       Date:  1982-05       Impact factor: 3.490

9.  Properties of S-adenosyl-L-methionine:macrocin O-methyltransferase in extracts of Streptomyces fradiae strains which produce normal or elevated levels of tylosin and in mutants blocked in specific O-methylations.

Authors:  E T Seno; R H Baltz
Journal:  Antimicrob Agents Chemother       Date:  1981-09       Impact factor: 5.191

10.  Intergeneric protoplast fusion between Fusobacterium varium and Enterococcus faecium for enhancing dehydrodivanillin degradation.

Authors:  W Chen; K Ohmiya; S Shimizu
Journal:  Appl Environ Microbiol       Date:  1987-03       Impact factor: 4.792
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