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Literature DB >> 400800

Fusion of yeast spheroplasts.

Abstract

Spheroplasts of two different auxotrophic strains of Saccharomyces cerevisiae, both of mating type a, were fused with the aid of polyethylene glycol and calcium ions. After reversion to vegetative cells in solid media, the resulting zygotes were shown to be diploid cells of mating type aa.

Entities: Chemical Species

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Year: 1977 PMID： 400800 PMCID： PMC235301 DOI： 10.1128/jb.130.2.946-947.1977

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

6 in total

1. Fusion of hen erythrocytes with yeast protoplasts induced by polyethylene glycol.

Authors: Q F Ahkong; J I Howell; J A Lucy; F Safwat; M R Davey; E C Cocking
Journal: Nature Date: 1975-05-01 Impact factor: 49.962

2. Physical conditions as important factors for the regeneration of naked yeast protoplasts.

Authors: O NECAS
Journal: Nature Date: 1961-11-11 Impact factor: 49.962

3. Purification and properties of an enzyme, zymolyase, which lyses viable yeast cells.

Authors: K Kitamura; Y Yamamoto
Journal: Arch Biochem Biophys Date: 1972-11 Impact factor: 4.013

4. Fusion of protoplasts of Bacillus megaterium.

Authors: K Fodor; L Alföldi
Journal: Proc Natl Acad Sci U S A Date: 1976-06 Impact factor: 11.205

5. Fusion of bacterial protoplasts.

Authors: P Schaeffer; B Cami; R D Hotchkiss
Journal: Proc Natl Acad Sci U S A Date: 1976-06 Impact factor: 11.205

6. Regeneration of yeast protoplasts in agar gels.

Authors: A Svoboda
Journal: Exp Cell Res Date: 1966 Nov-Dec Impact factor: 3.905

6 in total

38 in total

10. swi6, a gene required for mating-type switching, prohibits meiotic recombination in the mat2-mat3 "cold spot" of fission yeast.

Authors: A J Klar; M J Bonaduce
Journal: Genetics Date: 1991-12 Impact factor: 4.562

Fusion of yeast spheroplasts.

1. Fusion of hen erythrocytes with yeast protoplasts induced by polyethylene glycol.

2. Physical conditions as important factors for the regeneration of naked yeast protoplasts.

3. Purification and properties of an enzyme, zymolyase, which lyses viable yeast cells.

4. Fusion of protoplasts of Bacillus megaterium.

5. Fusion of bacterial protoplasts.

6. Regeneration of yeast protoplasts in agar gels.

1. Sister chromatids are preferred over homologs as substrates for recombinational repair in Saccharomyces cerevisiae.

2. Saccharomyces cerevisiae mutants that tolerate centromere plasmids at high copy number.

3. Hybridization and Polyploidization of Saccharomyces cerevisiae Strains by Transformation-Associated Cell Fusion.

4. Protoplast fusion in a petite-negative yeast, Kluyveromyces lactis.

5. Hybridization of Saccharomyces cerevisiae with Candida utilis through protoplast fusion.

6. Rapid genetic test for discrimination between haploid and polyploid transformants in Saccharomyces.

7. Production and genetic analysis of yeast cybrids.

8. Improvement of protoplast regeneration by growing yeast cells hypertonically.

9. Construction of lactose-assimilating and high-ethanol-producing yeasts by protoplast fusion.

10. swi6, a gene required for mating-type switching, prohibits meiotic recombination in the mat2-mat3 "cold spot" of fission yeast.