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Literature DB >> 4905701

Improved medium for detecting deoxyribonuclease-producing bacteria.

Abstract

Incorporation of methyl green dye into an agar medium containing deoxyribo-nucleic acid results in an improved medium for detecting deoxyribonuclease-producing bacteria. Use of the dye makes it unnecessary to use acid to demonstrate deoxyribonuclease activity, thus allowing subculture or reincubation of colonies. The improved medium is highly sensitive and can be used for primary isolation of Staphylococcus aureus or group A Streptococcus pyogenes.

Entities: Chemical Species

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Year: 1969 PMID： 4905701 PMCID： PMC378181 DOI： 10.1128/am.18.6.991-993.1969

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

7 in total

1. Rapid method for determining the activity of microorganisms on nucleic acids.

Authors: C D JEFFRIES; D F HOLTMAN; D G GUSE
Journal: J Bacteriol Date: 1957-04 Impact factor: 3.490

2. The determination of desoxyribonuclease activity by methyl green; application to serum.

Authors: N B KURNICK
Journal: Arch Biochem Date: 1950-11

3. Modification of deoxyribonuclease test medium for rapid identification of Serratia marcescens.

Authors: J B Schreier
Journal: Am J Clin Pathol Date: 1969-06 Impact factor: 2.493

4. Streptococcal anti-desoxyribonuclease B: microtechnique determination.

Authors: J Nelson; E M Ayoub; L W Wannamaker
Journal: J Lab Clin Med Date: 1968-05

5. Use of the deoxyribonuclease test as an aid in the differentiation of Paracolobactrum (Hafnia) from serratia.

Authors: J A Valu
Journal: J Bacteriol Date: 1966-01 Impact factor: 3.490

6. A new medium, salt mannitol plasma agar, for the isolation of Staphylococcus aureus.

Authors: E B Blair; J S Emerson; A H Tull
Journal: Am J Clin Pathol Date: 1967-01 Impact factor: 2.493

7. Detection of nuclease activity in semisolid and broth cultures.

Authors: R V Lachica; R H Deibel
Journal: Appl Microbiol Date: 1969-08

7 in total

30 in total

Review 1. Fluorogenic and chromogenic substrates used in bacterial diagnostics.

Authors: M Manafi; W Kneifel; S Bascomb
Journal: Microbiol Rev Date: 1991-09

2. Bacteriophage-associated genes responsible for the widely divergent phenotypes of variants of Burkholderia pseudomallei strain MSHR5848.

Authors: David DeShazer; Sean Lovett; Joshua Richardson; Galina Koroleva; Kathleen Kuehl; Kei Amemiya; Mei Sun; Patricia Worsham; Susan Welkos
Journal: J Med Microbiol Date: 2019-01-10 Impact factor: 2.472

Improved medium for detecting deoxyribonuclease-producing bacteria.

1. Rapid method for determining the activity of microorganisms on nucleic acids.

2. The determination of desoxyribonuclease activity by methyl green; application to serum.

3. Modification of deoxyribonuclease test medium for rapid identification of Serratia marcescens.

4. Streptococcal anti-desoxyribonuclease B: microtechnique determination.

5. Use of the deoxyribonuclease test as an aid in the differentiation of Paracolobactrum (Hafnia) from serratia.

6. A new medium, salt mannitol plasma agar, for the isolation of Staphylococcus aureus.

7. Detection of nuclease activity in semisolid and broth cultures.

Review 1. Fluorogenic and chromogenic substrates used in bacterial diagnostics.

2. Bacteriophage-associated genes responsible for the widely divergent phenotypes of variants of Burkholderia pseudomallei strain MSHR5848.

3. Deoxyribonuclease: detection with a three-hour test.

4. Presumptive identification of group A, B, and D streptococci on agar plate media.

5. Improved toluidine blue-DNA agar for detection of DNA hydrolysis by campylobacters.

6. Rapid deoxyribonuclease test with methyl green.

7. Turbidimetric assay of staphylococcal nuclease.

8. Selectagerm: a new approach to clinical bacteriology.

9. Phenotypic characteristics, fatty acid composition, and isoprenoid quinone content of CDC group IIg bacteria.

10. Rapid detection of staphylococcal thermonuclease on casings of naturally contaminated fermented sausages.