Literature DB >> 467436

Identification of the translation products of human fibroblast interferon mRNA in reticulocyte lysates.

J Weissenbach, M Zeevi, T Landau, M Revel.   

Abstract

Messenger RNA was purified from human foreskin fibroblasts FS11, a high interferon-producer line, after induction with synthetic double-stranded RNA. The mRNA was translated in a cell-free protein-synthesis system from rabbit reticulocytes. The translation products, containing biologically active human interferon, were immunoprecipitated by a serum from rabbits immunized against partially purified interferon. Analysis of the immunoprecipitate by polyacrylamide gel electrophoresis in dodecylsulfate shows that the product of human fibroblast interferon mRNA is a 23000-Mr polypeptide. Methods are described for the synthesis and rapid identification of this polypeptide, which should be useful for structural analysis of interferon and isolation of its mRNA.

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Year:  1979        PMID： 467436     DOI： 10.1111/j.1432-1033.1979.tb13153.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  15 in total

1.  Two interferon mRNAs in human fibroblasts: in vitro translation and Escherichia coli cloning studies.

Authors:  J Weissenbach; Y Chernajovsky; M Zeevi; L Shulman; H Soreq; U Nir; D Wallach; M Perricaudet; P Tiollais; M Revel
Journal:  Proc Natl Acad Sci U S A       Date:  1980-12       Impact factor: 11.205

2.  3' end structure of the human (2'-5') oligo A synthetase gene: prediction of two distinct proteins with cell type-specific expression.

Authors:  P Benech; G Merlin; M Revel; J Chebath
Journal:  Nucleic Acids Res       Date:  1985-02-25       Impact factor: 16.971

3.  A human IFN-beta 1 gene deleted of promoter sequences upstream from the TATA box is controlled post-transcriptionally by dsRNA.

Authors:  U Nir; B Cohen; L Chen; M Revel
Journal:  Nucleic Acids Res       Date:  1984-09-25       Impact factor: 16.971

4.  Interferon-induced 56,000 Mr protein and its mRNA in human cells: molecular cloning and partial sequence of the cDNA.

Authors:  J Chebath; G Merlin; R Metz; P Benech; M Revel
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971

5.  Interferon-dependent induction of mRNA for the major histocompatibility antigens in human fibroblasts and lymphoblastoid cells.

Authors:  M Fellous; U Nir; D Wallach; G Merlin; M Rubinstein; M Revel
Journal:  Proc Natl Acad Sci U S A       Date:  1982-05       Impact factor: 11.205

6.  Molecular cloning and sequence of partial cDNA for interferon-induced (2'-5')oligo(A) synthetase mRNA from human cells.

Authors:  G Merlin; J Chebath; P Benech; R Metz; M Revel
Journal:  Proc Natl Acad Sci U S A       Date:  1983-08       Impact factor: 11.205

7.  Nucleotide sequence and expression of human chromosome 21-encoded superoxide dismutase mRNA.

Authors:  L Sherman; N Dafni; J Lieman-Hurwitz; Y Groner
Journal:  Proc Natl Acad Sci U S A       Date:  1983-09       Impact factor: 11.205

8.  Human Cu/Zn superoxide dismutase gene: molecular characterization of its two mRNA species.

Authors:  L Sherman; D Levanon; J Lieman-Hurwitz; N Dafni; Y Groner
Journal:  Nucleic Acids Res       Date:  1984-12-21       Impact factor: 16.971

9.  Secretory proteins induced in human fibroblasts under conditions used for the production of interferon beta.

Authors:  J Content; L De Wit; D Pierard; R Derynck; E De Clercq; W Fiers
Journal:  Proc Natl Acad Sci U S A       Date:  1982-05       Impact factor: 11.205

10.  Presence of an abnormal beta 2-microglobulin mRNA in Daudi cells: induction by interferon.

Authors:  F Rosa; M Fellous; M Dron; M Tovey; M Revel
Journal:  Immunogenetics       Date:  1983       Impact factor: 2.846
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