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Literature DB >> 6164058

Two interferon mRNAs in human fibroblasts: in vitro translation and Escherichia coli cloning studies.

J Weissenbach, Y Chernajovsky, M Zeevi, L Shulman, H Soreq, U Nir, D Wallach, M Perricaudet, P Tiollais, M Revel.

Abstract

Two mRNA species that produce biologically active interferon were isolated from human fibroblasts and studied by size fractionation and cloning in Escherichia coli plasmid pBR322. The major fibroblast interferon (Hu IFN-beta 1) is coded for by the smaller of the two mRNAs, an 11S species, 900 nucleotides long, which in cell-free systems yields a 20,000 Mr protein. The second interferon mRNA species (Hu IFN-beta 2) is 14S, about 1300 nucleotides long, and codes for another protein of 23,000-26,000 Mr. The two interferon mRNAs do not cross-hybridize. Both are induced by poly(rI.rC), but IFN-beta 2 mRNA is induced to about 10% in cells by cycloheximide treatment alone whereas under these conditions IFN-beta 1 is not induced.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1980 PMID： 6164058 PMCID： PMC350459 DOI： 10.1073/pnas.77.12.7152

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

23 in total

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