Literature DB >> 4629650

Purification of mycobacterial deoxyribonucleic acid.

E B Hill, L G Wayne, W M Gross.   

Abstract

Impurities believed to be polysaccharides have been found in mycobacterial deoxyribonucleic acid (DNA) preparations. Agar-gel diffusion of the DNA preparations against concanavalin A indicated the presence of three polysaccharides and was used to follow the purification procedures. The polysaccharides appeared to be the same for all strains studied. Precipitation of DNA with cetyltrimethylammonium bromide was used to separate impurities from some DNA preparations. The presence of the contaminants was found to affect markedly the determination of the guanine plus cytosine content according to a method dependent on the ratio of absorbancies at 260 and 280 nm; the impurities did not affect the determination by the method of thermal denaturation. The presence of a DNA-polysaccharide complex is suggested.

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Year:  1972        PMID： 4629650      PMCID： PMC251527          DOI： 10.1128/jb.112.3.1033-1039.1972

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  10 in total

1.  The ultraviolet spectrum of deoxyribonucleic acids and their constituents.

Authors:  E FREDERICQ; A OTH; F FONTAINE
Journal:  J Mol Biol       Date:  1961-02       Impact factor: 5.469

2.  Fractionation of DNA on columns of poly-L-lysine supported on kieselguhr.

Authors:  C W Helleiner
Journal:  Can J Biochem       Date:  1969-12

3.  Fractionation of Bacillus subtilis DNA by use of poly-L-lysine kieselguhr columns.

Authors:  S R Ayad; J Blamire
Journal:  Biochem Biophys Res Commun       Date:  1968-02-15       Impact factor: 3.575

4.  Isolation of deoxyribonucleic acid from mycobacteria.

Authors:  L G Wayne; W M Gross
Journal:  J Bacteriol       Date:  1968-04       Impact factor: 3.490

5.  Interaction of concanavalin A with an arabinogalactan from the cell wall of Mycobacterium bovis.

Authors:  I J Goldstein; A Misaki
Journal:  J Bacteriol       Date:  1970-08       Impact factor: 3.490

6.  The reaction of Concanavalin-A with mycobacterial culture filtrates.

Authors:  T M Daniel; J J Wisnieski
Journal:  Am Rev Respir Dis       Date:  1970-05

7.  Protein-carbohydrate interaction. IX. Application of the quantitative hapten inhibition technique to polysaccharide-concanavalin A interaction. Some comments on the forces involved n concanavalin A-polysaccharide interaction.

Authors:  L L So; I J Goldstein
Journal:  J Immunol       Date:  1967-07       Impact factor: 5.422

8.  Isolation and analysis of the nucleic acids and polysaccharides from Clostridium welchii.

Authors:  G K Darby; A S Jones; J F Kennedy; R T Walker
Journal:  J Bacteriol       Date:  1970-07       Impact factor: 3.490

9.  Polysaccharides of Mycobacterium bovis Ushi 10, Mycobacterium smegmatis, Mycobacterium phlei, and Atypical Mycobacterium P1.

Authors:  I Azuma; M Ajisaka; Y Yamamura
Journal:  Infect Immun       Date:  1970-09       Impact factor: 3.441

10.  Base composition of deoxyribonucleic acid isolated from mycobacteria.

Authors:  L G Wayne; W M Gross
Journal:  J Bacteriol       Date:  1968-12       Impact factor: 3.490
  10 in total
  11 in total

1.  Concentration and detection of hepatitis A virus and rotavirus from shellfish by hybridization tests.

Authors:  Y J Zhou; M K Estes; X Jiang; T G Metcalf
Journal:  Appl Environ Microbiol       Date:  1991-10       Impact factor: 4.792

2.  Rapid lysis technique for mycobacterial species.

Authors:  S S Hurley; G A Splitter; R A Welch
Journal:  J Clin Microbiol       Date:  1987-11       Impact factor: 5.948

Review 3.  Mycobacterium.

Authors:  L Barksdale; K S Kim
Journal:  Bacteriol Rev       Date:  1977-03

4.  Isolation of deoxyribonucleic acid from Mycobacterium avium by rapid nitrogen decompression.

Authors:  P M Yandell; C McCarthy
Journal:  Infect Immun       Date:  1980-02       Impact factor: 3.441

5.  Genetic diversity and dynamic distribution of Mycobacterium tuberculosis isolates causing pulmonary and extrapulmonary tuberculosis in Thailand.

Authors:  Prapaporn Srilohasin; Angkana Chaiprasert; Katsushi Tokunaga; Nao Nishida; Therdsak Prammananan; Nat Smittipat; Surakameth Mahasirimongkol; Boonchai Chaiyasirinroje; Hideki Yanai; Prasit Palittapongarnpim
Journal:  J Clin Microbiol       Date:  2014-10-08       Impact factor: 5.948

6.  Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors.

Authors:  M D Chilton; T C Currier; S K Farrand; A J Bendich; M P Gordon; E W Nester
Journal:  Proc Natl Acad Sci U S A       Date:  1974-09       Impact factor: 11.205

7.  Attempts to detect deoxyribonucleic acid from Agrobacterium tumefaciens and bacteriophage PS8 in crown gall tumors by complementary ribonucleic acid-deoxyribonucleic acid-filter hybridization.

Authors:  F C Eden; S K Farrand; J S Powell; A J Bendich; M D Chilton; E W Nester; M P Gordon
Journal:  J Bacteriol       Date:  1974-08       Impact factor: 3.490

8.  Development of a diagnostic test for Johne's disease using a DNA hybridization probe.

Authors:  S S Hurley; G A Splitter; R A Welch
Journal:  J Clin Microbiol       Date:  1989-07       Impact factor: 5.948

9.  DNA isolated from Mycobacterium leprae: genome size, base ratio, and homology with other related bacteria as determined by optical DNA-DNA reassociation.

Authors:  T Imaeda; W F Kirchheimer; L Barksdale
Journal:  J Bacteriol       Date:  1982-04       Impact factor: 3.490

10.  Optimal DNA Isolation Method for Detection of Nontuberculous Mycobacteria by Polymerase Chain Reaction.

Authors:  Samira Mohammadi; Bahram Nasr Esfahani; Sharareh Moghim; Hossein Mirhendi; Fatemeh Riyahi Zaniani; Hajieh Ghasemian Safaei; Hossein Fazeli; Mahshid Salehi
Journal:  Adv Biomed Res       Date:  2017-10-25
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