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Literature DB >> 2447121

Rapid lysis technique for mycobacterial species.

Abstract

The lysis of mycobacterial cells typically has been difficult and time-consuming. We report a method for the physical rupture of Mycobacterium paratuberculosis and several other members of the family Mycobacteriaceae using a Mini-Beadbeater cell disrupter (Biospec Products; Bartlesville, Okla.) and zirconium beads, a process which yields DNA and RNA of high molecular weight and in greater quantity than that obtained by rupture in a Ribi pressure cell.

Entities: Species

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Year: 1987 PMID： 2447121 PMCID： PMC269451 DOI： 10.1128/jcm.25.11.2227-2229.1987

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

11 in total

1. Isolation of deoxyribonucleic acid from mycobacteria.

Authors: L G Wayne; W M Gross
Journal: J Bacteriol Date: 1968-04 Impact factor: 3.490

2. Method for isolation of deoxyribonucleic acid from mycobacteria.

Authors: Y Mizuguchi; T Tokunaga
Journal: J Bacteriol Date: 1970-11 Impact factor: 3.490

3. Deoxyribonucleic acid hybridization between different species of mycobacteria.

Authors: I Baess; M W Bentzon
Journal: Acta Pathol Microbiol Scand B Date: 1978-04

4. Isolation and analysis of the nucleic acids and polysaccharides from Clostridium welchii.

Authors: G K Darby; A S Jones; J F Kennedy; R T Walker
Journal: J Bacteriol Date: 1970-07 Impact factor: 3.490

5. Cloning of Mycobacterium bovis BCG DNA and expression of antigens in Escherichia coli.

Authors: J E Thole; H G Dauwerse; P K Das; D G Groothuis; L M Schouls; J D van Embden
Journal: Infect Immun Date: 1985-12 Impact factor: 3.441

6. Purification of mycobacterial deoxyribonucleic acid.

Authors: E B Hill; L G Wayne; W M Gross
Journal: J Bacteriol Date: 1972-12 Impact factor: 3.490

7. Relationships among mycobacteria and nocardiae based upon deoxyribonucleic acid reassociation.

Authors: S G Bradley
Journal: J Bacteriol Date: 1973-02 Impact factor: 3.490

8. Nucleic acid homology in the genus Mycobacterium.

Authors: W M Gross; L G Wayne
Journal: J Bacteriol Date: 1970-11 Impact factor: 3.490

9. Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae.

Authors: J E Clark-Curtiss; W R Jacobs; M A Docherty; L R Ritchie; R Curtiss
Journal: J Bacteriol Date: 1985-03 Impact factor: 3.490

10. DNA isolated from Mycobacterium leprae: genome size, base ratio, and homology with other related bacteria as determined by optical DNA-DNA reassociation.

Authors: T Imaeda; W F Kirchheimer; L Barksdale
Journal: J Bacteriol Date: 1982-04 Impact factor: 3.490

8 in total

1. A simple method for the isolation of chromosomal DNA from gram positive or acid-fast bacteria.

Authors: C Bollet; M J Gevaudan; X de Lamballerie; C Zandotti; P de Micco
Journal: Nucleic Acids Res Date: 1991-04-25 Impact factor: 16.971

2. Efficient differentiation of Mycobacterium avium complex species and subspecies by use of five-target multiplex PCR.

Authors: Sung Jae Shin; Byung Soo Lee; Won-Jung Koh; Elizabeth J B Manning; Kelly Anklam; Srinand Sreevatsan; Randall S Lambrecht; Michael T Collins
Journal: J Clin Microbiol Date: 2010-09-01 Impact factor: 5.948

3. Mechanical disruption of lysis-resistant bacterial cells by use of a miniature, low-power, disposable device.

Authors: Peter E Vandeventer; Kris M Weigel; Jose Salazar; Barbara Erwin; Bruce Irvine; Robert Doebler; Ali Nadim; Gerard A Cangelosi; Angelika Niemz
Journal: J Clin Microbiol Date: 2011-05-04 Impact factor: 5.948

4. Molecular cloning, expression, and immunogenicity of MTB12, a novel low-molecular-weight antigen secreted by Mycobacterium tuberculosis.

Authors: J R Webb; T S Vedvick; M R Alderson; J A Guderian; S S Jen; P J Ovendale; S M Johnson; S G Reed; Y A Skeiky
Journal: Infect Immun Date: 1998-09 Impact factor: 3.441

8. Performance evaluation of a dual linear ion trap-Fourier transform ion cyclotron resonance mass spectrometer for proteomics research.

Authors: Chad R Weisbrod; Michael R Hoopmann; Michael W Senko; James E Bruce
Journal: J Proteomics Date: 2013-04-13 Impact factor: 4.044