Literature DB >> 4612516

Isomeric phenylalanyl-tRNAs. Position of the aminoacyl moiety during protein biosynthesis.

S M Hecht, J W Kozarich, F J Schmidt.   

Abstract

The preparation of phenylalanyl-tRNA terminating in 3'-deoxyadenosine has been achieved by incubation of abbreviated tRNA (tRNA-CpC(OH)) with 3'-deoxyadenosine 5'-diphosphate and polynucleotide phosphorylase (EC 2.7.7.8), followed by aminoacylation. The isomeric phenylalanyl-tRNA terminating in 2'-deoxyadenosine was constructed by incubation of tRNA-CpC(OH) with 2'-deoxy-3'-O-phenylalanyladenosine 5'-diphosphate and polynucleotide phosphorylase. While tRNA is aminoacylated at the 2'-position, only the 3'-aminoacyl-tRNA is active as a peptide acceptor in the peptidyltransferase reaction. Both modified tRNAs were bound to the A-site as efficiently as unmodified tRNA, but neither was so efficient at P-site binding or as an acceptor in the peptidyltransferase reaction. Neither of the modified tRNAs acted as a donor in the peptidyltransferase reaction.

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Year:  1974        PMID： 4612516      PMCID： PMC433873          DOI： 10.1073/pnas.71.11.4317

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  15 in total

1.  In vitro in corporation of 2'-deoxyadenosine and 3'-deoxyadenosine into yeast tRNA Phe using t-RNA nucleotidyl transferase, and properties of tRNA Phe -C-C-2'dA and tRNA Phe -C-C-3'dA.

Authors:  M Sprinzl; K H Scheit; H Sternbach; F von der Haar; F Cramer
Journal:  Biochem Biophys Res Commun       Date:  1973-04-16       Impact factor: 3.575

2.  Terminal oxidation-reduction of yeast phenylalanine tRNA prevents donor and acceptor function at the peptidyl transferase center.

Authors:  Z Hussain; J Ofengand
Journal:  Biochem Biophys Res Commun       Date:  1973-02-20       Impact factor: 3.575

3.  Chemical modifications of transfer RNA species. Transfer RNA's terminating in 2'- and 3'-O-methyladenosine.

Authors:  S M Hecht; S D Hawrelak; J W Kozarich; F J Schmidt; R M Bock
Journal:  Biochem Biophys Res Commun       Date:  1973-06-19       Impact factor: 3.575

4.  Accepting site for aminoacylation of tRNAphe from yeast.

Authors:  M Sprinzl; F Cramer
Journal:  Nat New Biol       Date:  1973-09-05

5.  Chemical modifications of transfer rna species. Heavy atom derivatization of aminoacyl tRNA.

Authors:  F J Schmidt; R M Bock; S M Hecht
Journal:  Biochem Biophys Res Commun       Date:  1972-07-25       Impact factor: 3.575

6.  New approach to the synthesis of polyribonucleotides of defined sequence.

Authors:  J K Mackey; P T Gilham
Journal:  Nature       Date:  1971-10-22       Impact factor: 49.962

7.  Phosphorolysis of aminoacyl-tRNA by polynucleotide phosphorylase from Escherichia coli.

Authors:  G Kaufmann; U Z Littauer
Journal:  Eur J Biochem       Date:  1970-01

8.  Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns.

Authors:  R L Pearson; J F Weiss; A D Kelmers
Journal:  Biochim Biophys Acta       Date:  1971-02-11

9.  A proton exchange between purines and water and its application to biochemistry.

Authors:  K R Shelton; J M Clark
Journal:  Biochemistry       Date:  1967-09       Impact factor: 3.162

10.  The general synthetic route to amino acid esters of nucleotides and nucleoside-5'-triphosphates and some properties of these compounds.

Authors:  B P Gottikh; A A Krayevsky; N B Tarussova; P P Purygin; T L Tsilevich
Journal:  Tetrahedron       Date:  1970-09       Impact factor: 2.457
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  10 in total

Review 1.  Exploring the mechanism of protein synthesis with modified substrates and novel intermediate mimics.

Authors:  Joshua S Weinger; Scott A Strobel
Journal:  Blood Cells Mol Dis       Date:  2006-12-21       Impact factor: 3.039

2.  Participation of the tRNA A76 hydroxyl groups throughout translation.

Authors:  Joshua S Weinger; Scott A Strobel
Journal:  Biochemistry       Date:  2006-05-16       Impact factor: 3.162

3.  Properties of phenylalanine transfer ribonucleic acid with modified 3'-terminal end in protein biosynthesis using a rabbit reticulocyte cell-free system: effect of the replacement of cytidine residues from the CpCpA end of tRNA by 5-iodocytidine or 2-thiocytidine.

Authors:  E K Baksht; A Gal; N de Groot; A A Hochberg; M Sprinzl; F Cramer
Journal:  Nucleic Acids Res       Date:  1977-07       Impact factor: 16.971

Review 4.  What recent ribosome structures have revealed about the mechanism of translation.

Authors:  T Martin Schmeing; V Ramakrishnan
Journal:  Nature       Date:  2009-10-18       Impact factor: 49.962

5.  Position of aminoacylation of individual Escherichia coli and yeast tRNAs.

Authors:  S M Hecht; A C Chinualt
Journal:  Proc Natl Acad Sci U S A       Date:  1976-02       Impact factor: 11.205

6.  Isomeric aminoacyl-tRNAs are both bound by elongation factor Tu.

Authors:  S M Hecht; K H Tan; A C Chinault; P Arcari
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

7.  A Role for the 2' OH of peptidyl-tRNA substrate in peptide release on the ribosome revealed through RF-mediated rescue.

Authors:  Jeffrey J Shaw; Stefan Trobro; Shan L He; Johan Åqvist; Rachel Green
Journal:  Chem Biol       Date:  2012-08-24

8.  Interaction of elongation factor Tu with 2'(3')-O-aminoacyloligonucleotides derived from the 3' terminus of aminoacyl-tRNA.

Authors:  D Ringer; S Chládek
Journal:  Proc Natl Acad Sci U S A       Date:  1975-08       Impact factor: 11.205

Review 9.  A structural view on the mechanism of the ribosome-catalyzed peptide bond formation.

Authors:  Miljan Simonović; Thomas A Steitz
Journal:  Biochim Biophys Acta       Date:  2009-07-09

Review 10.  Chemistry of aminoacylation and peptide bond formation on the 3'terminus of tRNA.

Authors:  Mathias Sprinzl
Journal:  J Biosci       Date:  2006-10       Impact factor: 2.795
  10 in total

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