Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 RNA-linked DNA fragments in vitro.

Literature DB >> 4567338

RNA-linked DNA fragments in vitro.

Abstract

RNA-linked DNA fragments are intermediates in DNA replication in Escherichia coli cells made permeable to nucleoside triphosphates by treatment with toluene. Covalent linkage of a short RNA stretch to the 5' end of the DNA is proved by transfer of (32)P from [alpha-(32)P]dNTP to ribonucleotides upon digestion with alkali or pancreatic RNase, and by a small decrease in the molecular size upon alkaline hydrolysis. The (32)P transfer experiments reveal a unique structure...p(rPy)p(rA)p(rU or rC)p(dC)p... at the RNA-DNA junction.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 1973 PMID： 4567338 PMCID： PMC433190 DOI： 10.1073/pnas.70.1.88

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

15 in total

1. DEOXYTHYMIDINE KINASE OF ESCHERICHIA COLI. I. PURIFICATION AND SOME PROPERTIES OF THE ENZYME.

Authors: R OKAZAKI; A KORNBERG
Journal: J Biol Chem Date: 1964-01 Impact factor: 5.157

2. Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli.

Authors: I R LEHMAN; M J BESSMAN; E S SIMMS; A KORNBERG
Journal: J Biol Chem Date: 1958-07 Impact factor: 5.157

3. Initiation of DNA synthesis: synthesis of phiX174 replicative form requires RNA synthesis resistant to rifampicin.

Authors: R Schekman; W Wickner; O Westergaard; D Brutlag; K Geider; L L Bertsch; A Kornberg
Journal: Proc Natl Acad Sci U S A Date: 1972-09 Impact factor: 11.205

4. Evidence for the direct involvement of RNA in the initiation of DNA replication in Escherichia coli 15T.

Authors: K G Lark
Journal: J Mol Biol Date: 1972-02-28 Impact factor: 5.469

5. RNA-primed DNA synthesis in vitro.

Authors: W Keller
Journal: Proc Natl Acad Sci U S A Date: 1972-06 Impact factor: 11.205

6. Nucleotides at the RNA-DNA covalent bonds formed in the endogenous reaction by the avian myeloblastosis virus DNA polymerase.

Authors: R M Flügel; R D Wells
Journal: Virology Date: 1972-05 Impact factor: 3.616

7. Slow joining of newly replicated DNA chains in DNA polymerase I-deficient Escherichia coli mutants.

Authors: R Okazaki; M Arisawa; A Sugino
Journal: Proc Natl Acad Sci U S A Date: 1971-12 Impact factor: 11.205

8. RNA-linked nascent DNA fragments in Escherichia coli.

Authors: A Sugino; S Hirose; R Okazaki
Journal: Proc Natl Acad Sci U S A Date: 1972-07 Impact factor: 11.205

9. RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form.

Authors: W Wickner; D Brutlag; R Schekman; A Kornberg
Journal: Proc Natl Acad Sci U S A Date: 1972-04 Impact factor: 11.205

10. Isolation of supercoiled colicinogenic factor E 1 DNA sensitive to ribonuclease and alkali.

Authors: D G Blair; D J Sherratt; D B Clewell; D R Helinski
Journal: Proc Natl Acad Sci U S A Date: 1972-09 Impact factor: 11.205

13 in total

1. Nature of R-factor replication in the presence of chloramphenicol.

Authors: J H Crosa; L K Luttropp; S Falkow
Journal: Proc Natl Acad Sci U S A Date: 1975-02 Impact factor: 11.205

2. F deoxyribonucleic acid transferred to recipient cells in the presence of rifampin.

Authors: S Hiraga; T Saitoh
Journal: J Bacteriol Date: 1975-03 Impact factor: 3.490

3. RNA-primed DNA synthesis: specific catalysis by HeLa cell DNA polymerase alpha.

Authors: S Spadari; A Weissbach
Journal: Proc Natl Acad Sci U S A Date: 1975-02 Impact factor: 11.205

4. Molecular basis of a control mechanism of DNA synthesis in mammalian cells.

Authors: A M Novi
Journal: Klin Wochenschr Date: 1976-10-15

5. Role of polyadenylic acid in a deoxyribonucleic acid-membrane fraction extracted from pneumococci.

Authors: W Firshein; B Meyer; E Epner; J Viggiani
Journal: J Bacteriol Date: 1976-07 Impact factor: 3.490

6. Viral DNA-RNA hybrids in cells infected with simian virus: the simian virus 40 transcriptional intermediates.

Authors: M Girard; L Marty; S Manteuil
Journal: Proc Natl Acad Sci U S A Date: 1974-04 Impact factor: 11.205

7. Size and base composition of RNA in supercoiled plasmid DNA.

Authors: H Williams; H W Boyer; D R Helsinki
Journal: Proc Natl Acad Sci U S A Date: 1973-12 Impact factor: 11.205

8. Initiator RNA in discontinuous polyoma DNA synthesis.

Authors: P Reichard; R Eliasson; G Söderman
Journal: Proc Natl Acad Sci U S A Date: 1974-12 Impact factor: 11.205

9. The presence of ribonucleotides in mature closed-circular mitochondrial DNA.

Authors: L I Grossman; R Watson; J Vinograd
Journal: Proc Natl Acad Sci U S A Date: 1973-12 Impact factor: 11.205

10. RNA-DNA covalent bonds between the RNA primers and the DNA products formed by RNA tumor virus DNA polymerase.

Authors: R M Flügel; U Rapp; R D Wells
Journal: J Virol Date: 1973-12 Impact factor: 5.103