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Literature DB >> 4563980

High-level production of -galactosidase by Escherichia coli merodiploids.

Abstract

Two merodiploids of Escherichia coli that contain genes for the lac operon on both chromosome and episome were tested for production of lac enzymes after growth on various carbon sources. The specific activity of beta-galactosidase (and of thiogalactoside transacetylase) was about twice that from haploid cells when grown on glycerol. With succinate as carbon source, the specific activity increased by an additional factor of 3. Up to 25% of the soluble cell protein is beta-galactosidase in these strains, one of which is inducible and the other constitutive. The enzyme is purified easily in high yield by ammonium sulfate fractionation and electrophoresis.

Entities: Chemical Gene Species

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Year: 1972 PMID： 4563980 PMCID： PMC251496 DOI： 10.1128/jb.112.2.856-860.1972

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

15 in total

1. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

2. The amino acid sequence of -galactosidase. II. Tryptic peptides of the maleylated protein and sequences of some tryptic peptides.

Authors: A V Fowler
Journal: J Biol Chem Date: 1972-09-10 Impact factor: 5.157

3. The amino acid sequence of -galactosidase. 3. The sequences of NH 2 - and COOH-terminal tryptic peptides.

Authors: I Zabin; A V Fowler
Journal: J Biol Chem Date: 1972-09-10 Impact factor: 5.157

4. Beta-galactosidase. In vivo -complementation.

Authors: M Villarejo; P J Zamenhof; I Zabin
Journal: J Biol Chem Date: 1972-04-10 Impact factor: 5.157

5. Beta-galactosidase. Rates of synthesis and degradation of incomplete chains.

Authors: S Lin; I Zabin
Journal: J Biol Chem Date: 1972-04-10 Impact factor: 5.157

6. -Galactosidase: immunological activity of ribosome-bound, growing polypeptide chains.

Authors: J Hamlin; I Zabin
Journal: Proc Natl Acad Sci U S A Date: 1972-02 Impact factor: 11.205

7. The amino acid sequence of beta galactosidase. I. Isolation and composition of tryptic peptides.

Authors: A V Fowler; I Zabin
Journal: J Biol Chem Date: 1970-10-10 Impact factor: 5.157

8. Co-linearity of beta-galactosidase with its gene by immunological detection of incomplete polypeptide chains.

Authors: A V Fowler; I Zabin
Journal: Science Date: 1966-11-25 Impact factor: 47.728

9. Purification and characterization of the multiple forms of beta-galactosidase of Escherichia coli.

Authors: S L Marchesi; E Steers; S Shifrin
Journal: Biochim Biophys Acta Date: 1969-05

10. Nonsense mutants and polarity in the lac operon of Escherichia coli.

Authors: W A Newton; J R Beckwith; D Zipser; S Brenner
Journal: J Mol Biol Date: 1965-11 Impact factor: 5.469

17 in total

1. Molecular basis of beta-galactosidase alpha-complementation.

Authors: K E Langley; M R Villarejo; A V Fowler; P J Zamenhof; I Zabin
Journal: Proc Natl Acad Sci U S A Date: 1975-04 Impact factor: 11.205

2. beta-Galactosidase chimeras: primary structure of a lac repressor-beta-galactosidase protein.

Authors: A J Brake; A V Fowler; I Zabin; J Kania; B Müller-Hill
Journal: Proc Natl Acad Sci U S A Date: 1978-10 Impact factor: 11.205

3. Immunological screening method to detect specific translation products.

Authors: S Broome; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1978-06 Impact factor: 11.205

4. PDZ affinity chromatography: a general method for affinity purification of proteins based on PDZ domains and their ligands.

Authors: Ward G Walkup; Mary B Kennedy
Journal: Protein Expr Purif Date: 2014-03-06 Impact factor: 1.650

5. Temperature-sensitive catabolite activator protein in Escherichia coli BUG6.

Authors: D Benner; N Müller; W Boos
Journal: J Bacteriol Date: 1985-01 Impact factor: 3.490

6. Position of the mutation in beta-galactosidase ochre mutant U118.

Authors: I Zabin; A V Fowler; J R Beckwith
Journal: J Bacteriol Date: 1978-01 Impact factor: 3.490

7. Nascent polypeptide chains emerge from the exit domain of the large ribosomal subunit: immune mapping of the nascent chain.

Authors: C Bernabeu; J A Lake
Journal: Proc Natl Acad Sci U S A Date: 1982-05 Impact factor: 11.205