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Literature DB >> 412841

Position of the mutation in beta-galactosidase ochre mutant U118.

Abstract

The Escherichia coli lacZ ochre mutant strain U118 was converted to an amber mutant and suppressed with supF, which inserts tyrosine. Enzymatically active beta-galactosidase was isolated. It contained tyrosine at residue number 17 instead of glutamic acid as in wild type.

Entities: Chemical Gene Species

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Year: 1978 PMID： 412841 PMCID： PMC222035 DOI： 10.1128/jb.133.1.437-438.1978

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

9 in total

1. Amino acid sequence of beta-galactosidase. IV. Sequence of an alpha-complementing cyanogen bromide peptide, residues 3 to 92.

Authors: K E Langley; A V Fowler; I Zabin
Journal: J Biol Chem Date: 1975-04-10 Impact factor: 5.157

2. Genetic fusions defining trp and lac operon regulatory elements.

Authors: D H Mitchell; W S Reznikoff; J R Beckwith
Journal: J Mol Biol Date: 1975-04-15 Impact factor: 5.469

3. Molecular basis of beta-galactosidase alpha-complementation.

Authors: K E Langley; M R Villarejo; A V Fowler; P J Zamenhof; I Zabin
Journal: Proc Natl Acad Sci U S A Date: 1975-04 Impact factor: 11.205

4. The amino acid sequence of beta-galactosidase of Escherichia coli.

Authors: A V Fowler; I Zabin
Journal: Proc Natl Acad Sci U S A Date: 1977-04 Impact factor: 11.205

5. Lac repressor can be fused to beta-galactosidase.

Authors: B Müller-Hill; J Kania
Journal: Nature Date: 1974-06-07 Impact factor: 49.962

6. High-level production of -galactosidase by Escherichia coli merodiploids.

Authors: A V Fowler
Journal: J Bacteriol Date: 1972-11 Impact factor: 3.490

7. Amber suppression: a nucleotide change in the anticodon of a tyrosine transfer RNA.

Authors: H M Goodman; J Abelson; A Landy; S Brenner; J D Smith
Journal: Nature Date: 1968-03-16 Impact factor: 49.962

8. Nonsense mutants and polarity in the lac operon of Escherichia coli.

Authors: W A Newton; J R Beckwith; D Zipser; S Brenner
Journal: J Mol Biol Date: 1965-11 Impact factor: 5.469

9. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.

Authors: M J Casadaban
Journal: J Mol Biol Date: 1976-07-05 Impact factor: 5.469

9 in total

12 in total

Position of the mutation in beta-galactosidase ochre mutant U118.

1. Amino acid sequence of beta-galactosidase. IV. Sequence of an alpha-complementing cyanogen bromide peptide, residues 3 to 92.

2. Genetic fusions defining trp and lac operon regulatory elements.

3. Molecular basis of beta-galactosidase alpha-complementation.

4. The amino acid sequence of beta-galactosidase of Escherichia coli.

5. Lac repressor can be fused to beta-galactosidase.

6. High-level production of -galactosidase by Escherichia coli merodiploids.

7. Amber suppression: a nucleotide change in the anticodon of a tyrosine transfer RNA.

8. Nonsense mutants and polarity in the lac operon of Escherichia coli.

9. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.

1. Sites within gene lacZ of Escherichia coli for formation of active hybrid beta-galactosidase molecules.

2. beta-Galactosidase chimeras: primary structure of a lac repressor-beta-galactosidase protein.

Review 3. Uses of lac fusions for the study of biological problems.

4. Nonsense suppression in aminoacyl-t-RNA limited cells.

5. Observations on the formation of clones containing araB-lacZ cistron fusions.

6. Indirect stimulation of genetic recombination.

7. Mutations in the lacY gene of Escherichia coli define functional organization of lactose permease.

8. lambda Immunity phase shift in a lambda N- -lacZ+ fusion.

9. Positions of early nonsense and deletion mutations in lacZ.

10. Trans-acting RNA inhibits tRNA suppressor activity in vivo.