Literature DB >> 4554533

Covalent attachment of a peptidyl-transfer RNA analog to the 50S subunit of Escherichia coli ribosomes.

M Pellegrini, H Oen, C R Cantor.   

Abstract

The peptidyl-tRNA analog, N-bromo-acetyl-Phenylalanyl-tRNA(Phe) has been prepared. Its binding to the 70S ribosome of E. coli is totally dependent upon polyuridylic acid. The analog becomes covalently attached to the 50S particle. It is associated with only one protein fraction after polyacrylamide-gel separation of total 50S proteins. The analog also reacts with 23S ribosomal RNA or a protein that remains tightly bound to this RNA after treatment with LiCl-urea and sodium dodecyl sulfate. The analog can function as a peptidyl-tRNA for at least one peptide transfer, but it then inhibits further chain elongation. This result strongly suggests that this analog becomes covalently bound at the P-site of the ribosome.

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Year:  1972        PMID： 4554533      PMCID： PMC426576          DOI： 10.1073/pnas.69.4.837

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  20 in total

Review 1.  Selective chemical modification of proteins.

Authors:  E Shaw
Journal:  Physiol Rev       Date:  1970-04       Impact factor: 37.312

2.  On the catalytic center of peptidyl transfer: a part of the 50 S ribosome structure.

Authors:  T Staehelin; D M Maglott; R E Monro
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1969

3.  Structure and function of E. coli ribosomes. V. Reconstitution of functionally active 30S ribosomal particles from RNA and proteins.

Authors:  P Traub; M Nomura
Journal:  Proc Natl Acad Sci U S A       Date:  1968-03       Impact factor: 11.205

4.  The behaviour of acetylphenylalanyl soluble ribonucleic acid in polyphenylalanine synthesis.

Authors:  A L Haenni; F Chapeville
Journal:  Biochim Biophys Acta       Date:  1966-01-18

5.  A possible mechanism for initiation of protein synthesis.

Authors:  T Nakamoto; D Kalokofsky
Journal:  Proc Natl Acad Sci U S A       Date:  1966-03       Impact factor: 11.205

6.  Reconstitution of 50S ribosomal subunits from dissociated molecular components.

Authors:  M Nomura; V A Erdmann
Journal:  Nature       Date:  1970-11-21       Impact factor: 49.962

7.  Ribosome-catalysed peptidyl transfer: the polyphenylalanine system.

Authors:  B E Maden; R R Traut; R E Monro
Journal:  J Mol Biol       Date:  1968-07-28       Impact factor: 5.469

8.  Studies on rat liver phenylalanyl transfer ribonucleic acid synthetase. I. Purification, stabilization, and complex formation.

Authors:  K W Lanks; J Sciscenti; I B Weinstein; C R Cantor
Journal:  J Biol Chem       Date:  1971-06-10       Impact factor: 5.157

9.  Topography of the Escherichia coli ribosome. II. Preliminary sequence of 50 s subunit protein attack by trypsin and its correlation with functional activities.

Authors:  F N Chang; J G Flaks
Journal:  J Mol Biol       Date:  1971-10-28       Impact factor: 5.469

10.  The synthesis of N-acetylphenylalanyl-sRNA.

Authors:  N de Groot; Y Lapidot; A Panet; Y Wolman
Journal:  Biochem Biophys Res Commun       Date:  1966-10-05       Impact factor: 3.575
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  6 in total

1.  Proteins at the tRNA binding sites of Escherichia coli ribosomes.

Authors:  A P Czernilofsky; E E Collatz; G Stöffler; E Kuechler
Journal:  Proc Natl Acad Sci U S A       Date:  1974-01       Impact factor: 11.205

2.  Synthesis of cyclohexylpuromycin and its reaction with N-acetylphenylalanyl-transfer ribonucleic acid on rat liver ribosomes.

Authors:  M Ariatti; A O Hawtrey
Journal:  Biochem J       Date:  1975-02       Impact factor: 3.857

3.  Identification of 50S proteins at the peptidyl-tRNA binding site of Escherichia coli ribosomes.

Authors:  H Oen; M Pellegrini; D Eilat; C R Cantor
Journal:  Proc Natl Acad Sci U S A       Date:  1973-10       Impact factor: 11.205

4.  Electron microscopic visualization of tRNA genes with ferritin-avidin: biotin labels.

Authors:  T R Broker; L M Angerer; P H Yen; N D Hershey; N Davidson
Journal:  Nucleic Acids Res       Date:  1978-02       Impact factor: 16.971

5.  Mapping of Escherichia coli ribosomal components involved in peptidyl transferase activity.

Authors:  N Sonenberg; M Wilchek; A Zamir
Journal:  Proc Natl Acad Sci U S A       Date:  1973-05       Impact factor: 11.205

6.  Identification of chloramphenicol-binding protein in Escherichia coli ribosomes by affinity labeling.

Authors:  O Pongs; R Bald; V A Erdmann
Journal:  Proc Natl Acad Sci U S A       Date:  1973-08       Impact factor: 11.205
  6 in total

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