Literature DB >> 4530276

A limited number of globin genes in human DNA.

R Gambino, D Kacian, J O'Donnell, F Ramirez, P A Marks, A Bank.   

Abstract

The number of globin genes in human cells was determined by hybridizing DNA from human spleens to (3)H-labeled DNA complementary to human globin mRNA. Assuming the rates of reannealing of complementary DNA and cellular DNA are similar, the extent of hybridization of complementary DNA at various ratios of cellular DNA to complementary DNA indicate that there are fewer than 10 globin gene copies per haploid human genome. An alternative analysis of the data, which introduces no assumptions concerning the relative rates of reaction of complementary DNA and cellular DNA, indicates fewer than 20 globin gene copies are present. DNA isolated from the spleen of a patient with beta(+) thalassemia contained a number of globin gene copies similar to that of normal DNA.

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Year:  1974        PMID： 4530276      PMCID： PMC434307          DOI： 10.1073/pnas.71.10.3966

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  22 in total

1.  In vitro synthesis of DNA complementary to purified rabbit globin mRNA (RNA-dependent DNA polymerase-reticulocyte-hemoglobin-density gradient centrifugation-oligo(dT) primer).

Authors:  J Ross; H Aviv; E Scolnick; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1972-01       Impact factor: 11.205

2.  In vitro synthesis of DNA components of human genes for globins.

Authors:  A Bank; M Terada; S Metafora; L Dow; P A Marks
Journal:  Nat New Biol       Date:  1972-02-09

3.  In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA.

Authors:  I M Verma; G F Temple; H Fan; D Baltimore
Journal:  Nat New Biol       Date:  1972-02-09

4.  DNA-RNA hybridization in vast DNA excess.

Authors:  M Melli; C Whitfield; K V Rao; M Richardson; J O Bishop
Journal:  Nat New Biol       Date:  1971-05-05

5.  Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.

Authors:  R J Britten; D E Kohne
Journal:  Science       Date:  1968-08-09       Impact factor: 47.728

6.  Silent hemoglobin alpha genes in apes: potential source of thalassemia.

Authors:  S H Boyer; A N Noyes; G R Vrablik; L J Donaldson; E W Schaefer; C W Gray; T F Thurmon
Journal:  Science       Date:  1971-01-15       Impact factor: 47.728

7.  Evidence for multiple structural genes for the gamma chain of human fetal hemoglobin.

Authors:  W A Schroeder; T H Huisman; J R Shelton; J B Shelton; E F Kleihauer; A M Dozy; B Robberson
Journal:  Proc Natl Acad Sci U S A       Date:  1968-06       Impact factor: 11.205

8.  Isolation and translation of hemoglobin messenger RNA from thalassemia, sickle cell anemia, and normal human reticulocytes.

Authors:  A W Nienhuis; W F Anderson
Journal:  J Clin Invest       Date:  1971-11       Impact factor: 14.808

9.  Isolation of deoxyribonucleic acid from mammalian tissues.

Authors:  K S Kirby; E A Cook
Journal:  Biochem J       Date:  1967-07       Impact factor: 3.857

10.  Defect in messenger RNA for human hemoglobin synthesis in beta thalassemia.

Authors:  E J Benz; B G Forget
Journal:  J Clin Invest       Date:  1971-12       Impact factor: 14.808
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  8 in total

1.  Study of markers of human erythroid differentiation in hybrid cells.

Authors:  A Deisseroth; A Nienhuis
Journal:  In Vitro       Date:  1976-11

2.  Quantitation of human gamma globin genes and gamma globin mRNA with purified gamma globin complementary DNA.

Authors:  F Ramirez; J V O'Donnell; C Natta; A Bank
Journal:  J Clin Invest       Date:  1976-12       Impact factor: 14.808

3.  Defects in DNA and globin messenger RNA in homozygotes for hemoglobin Lepore.

Authors:  F Ramirez; J G Mears; U Nudel; A Bank; L Luzzatto; G DiPrisco; R D'Avino; G Pepe; L Camardella; R Gambino; R Cimino; N Quattrin
Journal:  J Clin Invest       Date:  1979-04       Impact factor: 14.808

4.  No detectable reiteration of genes coding for mouse MOPC 41 immunoglobulin light-chain mRNA.

Authors:  M G Farace; M F Aellen; P A Briand; C H Faust; P Vassalli; B Mach
Journal:  Proc Natl Acad Sci U S A       Date:  1976-03       Impact factor: 11.205

5.  Human globin gene analysis for a patient with beta-o/delta beta-thalassemia.

Authors:  S Ottolenghi; W G Lanyon; R Williamson; D J Weatherall; J B Clegg; C S Pitcher
Journal:  Proc Natl Acad Sci U S A       Date:  1975-06       Impact factor: 11.205

6.  Changes in restricted human cellular DNA fragments containing globin gene sequences in thalassemias and related disorders.

Authors:  J G Mears; F Ramirez; D Leibowitz; F Nakamura; A Bloom; F Konotey-Ahulu; A Bank
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

7.  Human placental lactogen mRNA and its structural genes during pregnancy: quantitation with a complementary DNA.

Authors:  D McWilliams; R C Callahan; I Boime
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205

8.  Relative numbers of human globin genes assayed with purified alpha and beta complementary human DNA.

Authors:  F Ramirez; C Natta; J V O'Donnell; V Canale; G Bailey; T Sanguensermsri; G M Maniatis; P A Marks; A Bank
Journal:  Proc Natl Acad Sci U S A       Date:  1975-04       Impact factor: 11.205
  8 in total

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