Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Restriction and modification in B. subtilis. Purification and general properties of a restriction endonuclease from strain R.

Literature DB >> 2856

Restriction and modification in B. subtilis. Purification and general properties of a restriction endonuclease from strain R.

Abstract

All Bacillus subtilis R-type strains showing the phenomena of restriction and modification contain an endonuclease that inactivates in vitro the biological activity of a variety of DNAs lacking R-specific modification, such as transfecting SPPI, SPO2 and phi105 DNA, and transforming B. subtilis 168-type DNA. The corresponding DNAs carrying R-specific modification are resistant to the enzyme. The enzyme has been purified approximately 400-fold and is essentially free from contaminating double strand-directed unspecific exo- or endonuclease activity. Only Mg2+ is required as cofactor. The substrate DNAs are cleaved at specific sites. The double-stranded fragments produced from SPP1 DNA (molecular weight 2.5 x 10(7)) have an average molecular weight of about 3 x 10(5).

Entities: Chemical Species

Mesh：

Substances：

Year: 1975 PMID： 2856 DOI： 10.1007/BF00269416

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

40 in total

1. HOST SPECIFICITY OF DNA PRODUCED BY ESCHERICHIA COLI. IV. HOST SPECIFICITY OF INFECTIOUS DNA FROM BACTERIOPHAGE LAMBDA.

Authors: D DUSSOIX; W ARBER
Journal: J Mol Biol Date: 1965-02 Impact factor: 5.469

2. Nucleotide sequences at the cleavage sites of two restriction endonucleases from Hemophilus parainfluenzae.

Authors: D E Garfin; H M Goodman
Journal: Biochem Biophys Res Commun Date: 1974-07-10 Impact factor: 3.575

3. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors: P A Sharp; B Sugden; J Sambrook
Journal: Biochemistry Date: 1973-07-31 Impact factor: 3.162

4. Transfecting deoxyribonucleic acid of Bacillus bacteriophage phi 29 that is protease sensitive.

Authors: H Hirokawa
Journal: Proc Natl Acad Sci U S A Date: 1972-06 Impact factor: 11.205

5. Physical properties of the DNA of bacteriophage SP50.

Authors: N Biswal; A K Kleinschmidt; H C Spatz; T A Trautner
Journal: Mol Gen Genet Date: 1967

Review 6. DNA modification and restriction.

Authors: W Arber; S Linn
Journal: Annu Rev Biochem Date: 1969 Impact factor: 23.643

7. Restriction of infectious bacteriophage fd DNA's and an assay for in vitro host-controlled restriction and modification.

Authors: R Benzinger
Journal: Proc Natl Acad Sci U S A Date: 1968-04 Impact factor: 11.205

8. Genetic and transfection studies with B, subtilis phage SP 50. I. Phage mutants with restricted growth on B. subtilis strain 168.

Authors: E Rottländer; T A Trautner
Journal: Mol Gen Genet Date: 1970

9. Intergenotic transformation of the Bacillus subtilis genospecies.

Authors: G A Wilson; F E Young
Journal: J Bacteriol Date: 1972-09 Impact factor: 3.490

10. Action of haemophilus endodeoxyribonuclease on biologically active deoxyribonucleic acid.

Authors: R Gromkova; S H Goodgal
Journal: J Bacteriol Date: 1972-03 Impact factor: 3.490

49 in total

1. Introduction of host-controlled modification and restriction systems of Bacillus subtilis IAM1247 into Bacillus subtilis 168.

Authors: T Shibata; S Ikawa; Y Komatsu; T Ando; H Saito
Journal: J Bacteriol Date: 1979-07 Impact factor: 3.490

Restriction and modification in B. subtilis. Purification and general properties of a restriction endonuclease from strain R.

1. HOST SPECIFICITY OF DNA PRODUCED BY ESCHERICHIA COLI. IV. HOST SPECIFICITY OF INFECTIOUS DNA FROM BACTERIOPHAGE LAMBDA.

2. Nucleotide sequences at the cleavage sites of two restriction endonucleases from Hemophilus parainfluenzae.

3. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

4. Transfecting deoxyribonucleic acid of Bacillus bacteriophage phi 29 that is protease sensitive.

5. Physical properties of the DNA of bacteriophage SP50.

Review 6. DNA modification and restriction.

7. Restriction of infectious bacteriophage fd DNA's and an assay for in vitro host-controlled restriction and modification.

8. Genetic and transfection studies with B, subtilis phage SP 50. I. Phage mutants with restricted growth on B. subtilis strain 168.

9. Intergenotic transformation of the Bacillus subtilis genospecies.

10. Action of haemophilus endodeoxyribonuclease on biologically active deoxyribonucleic acid.

1. Introduction of host-controlled modification and restriction systems of Bacillus subtilis IAM1247 into Bacillus subtilis 168.

2. Genetic recombination during transformation in Bacillus subtilis: appearance of a deoxyribonucleic acid methylase.

Review 3. Transfection of Enterobacteriaceae and its applications.

4. The genome of Bacillus subtilis phage SPP1: the arrangement of restriction endonuclease generated fragments.

5. Restriction endonucleases: general survey procedure and survey of gliding bacteria.

6. Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

7. A restriction endonuclease from Staphylococcus aureus.

8. Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.

9. Site-specific deoxyribonucleases in Bacillus subtilis and other Bacillus strains.

10. Restriction and modification in Bacillus subtilis: inducibility of a DNA methylating activity in nonmodifying cells.