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Literature DB >> 4528644

Methylated messenger RNA synthesis in vitro by purified reovirus.

Abstract

Purified human reovirus contains RNA methylase activity in addition to an RNA polymerase. Virions incubated under appropriate conditions in the presence of S-adenosyl-L-methionine synthesize mRNA that is specifically methylated in the 5'-terminal guanosine. Alkaline digestion of the methylated RNA released a 5'-terminal dinucleotide, ppG'pCp, indicating that the guanosine contains 2'-O-methylribose. The possible roles of methylation in viral and cellular mRNA function is discussed.

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Year: 1974 PMID： 4528644 PMCID： PMC388651 DOI： 10.1073/pnas.71.8.3204

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

16 in total

1. "Methylation-coupled" transcription by virus-associated transcriptase of cytoplasmic polyhedrosis virus containing double-stranded RNA.

Authors: Y Furuichi
Journal: Nucleic Acids Res Date: 1974-06 Impact factor: 16.971

2. Initiation of reovirus mRNA synthesis in vitro.

Authors: A K Banerjee; R Ward; A J Shatkin
Journal: Nat New Biol Date: 1971-04-07

3. Polypeptide components of virions, top component and cores of reovirus type 3.

Authors: R E Smith; H J Zweerink; W K Joklik
Journal: Virology Date: 1969-12 Impact factor: 3.616

4. RNA polymerase activity in purified reoviruses.

Authors: A J Shatkin; J D Sipe
Journal: Proc Natl Acad Sci U S A Date: 1968-12 Impact factor: 11.205

5. Studies on the in vitro transcription of reovirus RNA catalyzed by reovirus cores.

Authors: J J Skehel; W K Joklik
Journal: Virology Date: 1969-12 Impact factor: 3.616

6. Transcription in vitro by reovirus-associated ribonucleic acid-dependent polymerase.

Authors: A K Banerjee; A J Shatkin
Journal: J Virol Date: 1970-07 Impact factor: 5.103

7. Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis.

Authors: A J Shatkin; J D Sipe; P Loh
Journal: J Virol Date: 1968-10 Impact factor: 5.103

8. Reovirus: RNA polymerase activity in purified virions.

Authors: J Borsa; A F Graham
Journal: Biochem Biophys Res Commun Date: 1968-12-30 Impact factor: 3.575

9. Asynchronous synthesis of the complementary strands of the reovirus genome.

Authors: M Schonberg; S C Silverstein; D H Levin; G Acs
Journal: Proc Natl Acad Sci U S A Date: 1971-02 Impact factor: 11.205

10. Properties of RNA transcriptase in reovirus subviral particles.

Authors: D H Levin; N Mendelsohn; M Schonberg; H Klett; S Silverstein; A M Kapuler; G Acs
Journal: Proc Natl Acad Sci U S A Date: 1970-07 Impact factor: 11.205

45 in total

1. Reovirus nonstructural protein muNS binds to core particles but does not inhibit their transcription and capping activities.

Authors: T J Broering; A M McCutcheon; V E Centonze; M L Nibert
Journal: J Virol Date: 2000-06 Impact factor: 5.103

Methylated messenger RNA synthesis in vitro by purified reovirus.

1. "Methylation-coupled" transcription by virus-associated transcriptase of cytoplasmic polyhedrosis virus containing double-stranded RNA.

2. Initiation of reovirus mRNA synthesis in vitro.

3. Polypeptide components of virions, top component and cores of reovirus type 3.

4. RNA polymerase activity in purified reoviruses.

5. Studies on the in vitro transcription of reovirus RNA catalyzed by reovirus cores.

6. Transcription in vitro by reovirus-associated ribonucleic acid-dependent polymerase.

7. Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis.

8. Reovirus: RNA polymerase activity in purified virions.

9. Asynchronous synthesis of the complementary strands of the reovirus genome.

10. Properties of RNA transcriptase in reovirus subviral particles.

1. Reovirus nonstructural protein muNS binds to core particles but does not inhibit their transcription and capping activities.

2. m7G5'ppp5'GmptcpUp at the 5' terminus of reovirus messenger RNA.

3. A protein binding the methylated 5'-terminal sequence, m7GpppN, of eukaryotic messenger RNA.

4. Reovirus messenger RNA contains a methylated, blocked 5'-terminal structure: m-7G(5')ppp(5')G-MpCp-.

5. 5'-Terminal m-7G(5')ppp(5')G-m-p in vivo: identification in reovirus genome RNA.

6. Methylated nucleotides block 5'-terminus of vaccinia virus messenger RNA.

7. Methylation-dependent translation of viral messenger RNAs in vitro.

8. Blocked and unblocked 5' termini in reovirus genome RNA.

9. Identification of the 5' end of Rous sarcoma virus RNA.

10. Nucleotide sequences at the 5' termini of reovirus mRNA's.