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Literature DB >> 5723704

Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis.

Abstract

Double-stranded ribonucleic acid (RNA) extracted from purified reoviruses of all three serotypes and from type 3 virus-infected cells was analyzed by polyacrylamide gel electrophoresis. It was calculated that each RNA includes 10 segments: 3 large, 3 intermediate, and 4 small fragments corresponding to molecular weights of about 2.5, 1.4, and 0.8 x 10(6) daltons, respectively, or a total of 15 x 10(6) daltons.

Entities: Chemical Species

Mesh：

Substances：
Acrylates
Gels
RNA, Viral

Year: 1968 PMID： 5723704 PMCID： PMC375428

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

22 in total

1. ELECTRON MICROSCOPE STUDIES ON REOVIRUS RNA.

Authors: P J GOMATOS; W STOECKENIUS
Journal: Proc Natl Acad Sci U S A Date: 1964-12 Impact factor: 11.205

2. Inactivity of purified reovirus RNA as a template for E. coli polymerases in vitro.

Authors: A J Shatkin
Journal: Proc Natl Acad Sci U S A Date: 1965-12 Impact factor: 11.205

3. Device for sectioning of polyacrylamide gel cylinders and its use in determining biological activity in the sections.

Authors: A Chrambach
Journal: Anal Biochem Date: 1966-06 Impact factor: 3.365

4. Electrophoretic separation of viral nucleic acids on polyacrylamide gels.

Authors: D H Bishop; J R Claybrook; S Spiegelman
Journal: J Mol Biol Date: 1967-06-28 Impact factor: 5.469

5. Studies on the A-rich RNA of reovirus.

Authors: A R Bellamy; W K Joklik
Journal: Proc Natl Acad Sci U S A Date: 1967-10 Impact factor: 11.205

6. Single-stranded, adenine-rich RNA from purified reoviruses.

Authors: A J Shatkin; J D Sipe
Journal: Proc Natl Acad Sci U S A Date: 1968-01 Impact factor: 11.205

7. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.

Authors: A C Peacock; C W Dingman
Journal: Biochemistry Date: 1967-06 Impact factor: 3.162

8. Studies on reovirus RNA. II. Characterization of reovirus messenger RNA and of the genome RNA segments from which it is transcribed.

Authors: A R Bellamy; W K Joklik
Journal: J Mol Biol Date: 1967-10-14 Impact factor: 5.469

9. Studies on reovirus RNA. I. Characterization of reovirus genome RNA.

Authors: A R Bellamy; L Shapiro; J T August; W K Joklik
Journal: J Mol Biol Date: 1967-10-14 Impact factor: 5.469

10. Structural units of reovirus ribonucleic acid and their possible functional significance.

Authors: Y Watanabe; A F Graham
Journal: J Virol Date: 1967-08 Impact factor: 5.103

93 in total

1. Control of transcription of the reovirus genome.

Authors: M Nonoyama; S Millward; A F Graham
Journal: Nucleic Acids Res Date: 1974-03 Impact factor: 16.971

2. Atomic force microscopy of reovirus dsRNA: a routine technique for length measurements.

Authors: Y L Lyubchenko; B L Jacobs; S M Lindsay
Journal: Nucleic Acids Res Date: 1992-08-11 Impact factor: 16.971

3. Complementation of defective reovirus by ts mutants.

Authors: D A Spandidos; A F Graham
Journal: J Virol Date: 1975-04 Impact factor: 5.103

4. Blocked and unblocked 5' termini in reovirus genome RNA.

Authors: N L Chow; A J Shatkin
Journal: J Virol Date: 1975-05 Impact factor: 5.103

5. Defective Interference in the Killer System of Saccharomyces cerevisiae.

Authors: S P Ridley; R B Wickner
Journal: J Virol Date: 1983-02 Impact factor: 5.103

6. Structural and growth characteristics of two avian reoviruses.

Authors: H Nick; D Cursiefen; H Becht
Journal: Arch Virol Date: 1975 Impact factor: 2.574

7. Functional expression and RNA binding analysis of the interferon-induced, double-stranded RNA-activated, 68,000-Mr protein kinase in a cell-free system.

Authors: M G Katze; M Wambach; M L Wong; M Garfinkel; E Meurs; K Chong; B R Williams; A G Hovanessian; G N Barber
Journal: Mol Cell Biol Date: 1991-11 Impact factor: 4.272