Literature DB >> 450716

Antibiotic induced electrophoretic mobility shifts of DNA restriction fragments.

E Loucks, G Chaconas, R W Blakesley, R D Wells, J H van de Sande.   

Abstract

Several antibiotics, netropsin, distamycin A, actinomycin D, Hoechst 33258 and olivomycin, which demonstrate base specificity in their DNA binding properties have been found to alter the electrophoretic mobility of DNA restriction fragments in native polyacrylamide gels. The antibiotics mostly reduced the migration of larger DNA fragments, but netropsin and Hoechst 33258 were observed to increase the migration rate of several DNA fragments of intermediate size. DNA fragments of similar molecular weight which comigrate as a single gel band can at times be separated as the result of differential mobility shifts promoted by antibiotic DNA complex formations.

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Year:  1979        PMID： 450716      PMCID： PMC327817          DOI： 10.1093/nar/6.5.1869

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  20 in total

1.  ACTINOMYCIN BINDING TO DNA: MECHANISM AND SPECIFICITY.

Authors:  M GELLERT; C E SMITH; D NEVILLE; G FELSENFELD
Journal:  J Mol Biol       Date:  1965-03       Impact factor: 5.469

2.  Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis.

Authors:  R C Parker; R M Watson; J Vinograd
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205

3.  End labeling of enzymatically decapped mRNA.

Authors:  A Efstratiadis; J N Vournakis; H Donis-Keller; G Chaconas; D K Dougall; F C Kafatos
Journal:  Nucleic Acids Res       Date:  1977-12       Impact factor: 16.971

4.  Nucleotide sequence of bacteriophage phi X174 DNA.

Authors:  F Sanger; G M Air; B G Barrell; N L Brown; A R Coulson; C A Fiddes; C A Hutchison; P M Slocombe; M Smith
Journal:  Nature       Date:  1977-02-24       Impact factor: 49.962

Review 5.  The role of DNA structure in genetic regulation.

Authors:  R D Wells; R W Blakesley; S C Hardies; G T Horn; J E Larson; E Selsing; J F Burd; H W Chan; J B Dodgson; K F Jensen; I F Nes; R M Wartell
Journal:  CRC Crit Rev Biochem       Date:  1977

6.  Duplex regions in "single-stranded" phiX174 DNA are cleaved by a restriction endonuclease from Haemophilus aegyptius.

Authors:  R W Blakesley; J B Dodgson; I F Nes; R D Wells
Journal:  J Biol Chem       Date:  1977-10-25       Impact factor: 5.157

7.  Visualization of drug-nucleic acid interactions at atomic resolution. III. Unifying structural concepts in understanding drug-DNA interactions and their broader implications in understanding protein-DNA interactions.

Authors:  H M Sobell; C C Tsai; S C Jain; S G Gilbert
Journal:  J Mol Biol       Date:  1977-08-15       Impact factor: 5.469

8.  Use of a sequence-specific DNA-binding ligand to probe the environments of EcoRI restriction endonuclease cleavage sites.

Authors:  J Kania; T G Fanning
Journal:  Eur J Biochem       Date:  1976-08-16

9.  Netropsin-poly(dA-dT) complex in solution: structure and dynamics of antibiotic-free base pair regions and those centered on bound netropsin.

Authors:  D J Patel; L L Canuel
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  The use of netropsin with CsCl gradients for the analysis of DNA and its application to restriction nuclease fragments of ribosomal DNA from Physarum polycephalum.

Authors:  H R Matthews; E M Johnson; W M Steer; E M Bradbury; V G Allfrey
Journal:  Eur J Biochem       Date:  1978-01-16
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  3 in total

1.  SPLINT: a cubic spline interpolation program for the analysis of fragment sizes in one-dimensional electrophoresis gels.

Authors:  C E Gariepy; M I Lomax; L I Grossman
Journal:  Nucleic Acids Res       Date:  1986-01-10       Impact factor: 16.971

2.  Human immunodeficiency virus type 1 DNA sequences genetically damaged by hypermutation are often abundant in patient peripheral blood mononuclear cells and may be generated during near-simultaneous infection and activation of CD4(+) T cells.

Authors:  M Janini; M Rogers; D R Birx; F E McCutchan
Journal:  J Virol       Date:  2001-09       Impact factor: 5.103

3.  Polyethylene glycol derivatives of base and sequence specific DNA ligands: DNA interaction and application for base specific separation of DNA fragments by gel electrophoresis.

Authors:  W Müller; I Hattesohl; H J Schuetz; G Meyer
Journal:  Nucleic Acids Res       Date:  1981-01-10       Impact factor: 16.971
  3 in total

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