Literature DB >> 202926

End labeling of enzymatically decapped mRNA.

A Efstratiadis, J N Vournakis, H Donis-Keller, G Chaconas, D K Dougall, F C Kafatos.   

Abstract

A method is presented for rapid and efficient 5' end labeling with 32P of capped mRNAs, by a series of three enzymatic reactions: the blocking nucleotide of the cap structure is removed by tobacco acid pyrophosphatase, and after dephosphorylation with alkaline phosphatase the 5' end is labeled with gamma-32-P-ATP and T4 polynucleotide kinase.

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Year:  1977        PMID： 202926      PMCID： PMC343232          DOI： 10.1093/nar/4.12.4165

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  19 in total

1.  Enzyme cleaving the 5'-terminal methylated blocked structure of messenger RNA.

Authors:  H Shinshi; M Miwa; T Sugimura
Journal:  FEBS Lett       Date:  1976-06-01       Impact factor: 4.124

2.  Effect of salts and polyamines on T4 polynucleotide kinase.

Authors:  J R Lillehaug; K Kleppe
Journal:  Biochemistry       Date:  1975-03-25       Impact factor: 3.162

3.  Importance of 5'-terminal blocking structure to stabilize mRNA in eukaryotic protein synthesis.

Authors:  K Shimotohno; Y Kodama; J Hashimoto; K I Miura
Journal:  Proc Natl Acad Sci U S A       Date:  1977-07       Impact factor: 11.205

4.  Enzymatic removal of the 5'-terminal methylated blocked structure of tobacco mosaic virus RNA and its effects on infectivity and reconstitution with coat protein.

Authors:  T Ohno; Y Okada; K Shimotohno; K Miura; H Shinshi
Journal:  FEBS Lett       Date:  1976-08-15       Impact factor: 4.124

5.  Nucleotide sequences at the 5'termini of rabbit alpha and beta globin mRNA.

Authors:  R E Lockhard; U L Rajbhandary
Journal:  Cell       Date:  1976-12       Impact factor: 41.582

6.  A novel phosphodiesterase from cultured tobacco cells.

Authors:  H Shinshi; M Miwa; K Kato; M Noguchi; T Matsushima; T Sugimura
Journal:  Biochemistry       Date:  1976-05-18       Impact factor: 3.162

7.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

8.  The primary structure of rabbit beta-globin mRNA as determined from cloned DNA.

Authors:  A Efstratiadis; F C Kafatos; T Maniatis
Journal:  Cell       Date:  1977-04       Impact factor: 41.582

9.  Translation of enzymically decapped messenger RNA.

Authors:  K A Abraham; A Pihl
Journal:  Eur J Biochem       Date:  1977-08-01

10.  Mapping adenines, guanines, and pyrimidines in RNA.

Authors:  H Donis-Keller; A M Maxam; W Gilbert
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971
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  73 in total

1.  Analysis of the structure of human telomerase RNA in vivo.

Authors:  Mária Antal; Eva Boros; Ferenc Solymosy; Tamás Kiss
Journal:  Nucleic Acids Res       Date:  2002-02-15       Impact factor: 16.971

2.  Sequence of 1000 nucleotides at the 3' end of tobacco mosaic virus RNA.

Authors:  H Guilley; G Jonard; B Kukla; K E Richards
Journal:  Nucleic Acids Res       Date:  1979-04       Impact factor: 16.971

3.  An antisense approach to phenotype-based gene cloning in Tetrahymena.

Authors:  N D Chilcoat; N C Elde; A P Turkewitz
Journal:  Proc Natl Acad Sci U S A       Date:  2001-07-03       Impact factor: 11.205

4.  Organization and transient expression of the gene for human U11 snRNA.

Authors:  C Suter-Crazzolara; W Keller
Journal:  Gene Expr       Date:  1991-05

5.  A family of wheat embryo U2 snRNAs.

Authors:  J M Skuzeski; J J Jendrisak
Journal:  Plant Mol Biol       Date:  1985-03       Impact factor: 4.076

6.  Evidence that a capped oligoribonucleotide is the primer for duck hepatitis B virus plus-strand DNA synthesis.

Authors:  J M Lien; C E Aldrich; W S Mason
Journal:  J Virol       Date:  1986-01       Impact factor: 5.103

7.  Analysis of the genome of an endogenous, ecotropic retrovirus of the AKR strain of mice: micromethod for detailed characterization of high-molecular-weight RNA.

Authors:  F S Pedersen; W A Haseltine
Journal:  J Virol       Date:  1980-01       Impact factor: 5.103

8.  Concurrent sequence analysis of 5' and 3' RNA termini by intramolecular circularization reveals 5' nontemplated bases and 3' terminal heterogeneity for lymphocytic choriomeningitis virus mRNAs.

Authors:  B J Meyer; P J Southern
Journal:  J Virol       Date:  1993-05       Impact factor: 5.103

9.  Differential expression of selected genes in human leukemia leukocytes.

Authors:  T Shiosaka; G F Saunders
Journal:  Proc Natl Acad Sci U S A       Date:  1982-08       Impact factor: 11.205

10.  Propagation of restriction fragments from the mitochondrial DNA of Saccharomyces cerevisiae in E. coli by means of plasmid vectors.

Authors:  P E Berg; A Lewin; T Christianson; M Rabinowitz
Journal:  Nucleic Acids Res       Date:  1979       Impact factor: 16.971
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