Literature DB >> 4501582

Characterization of messenger ribonucleoprotein and messenger RNA from KB cells.

A Kumar, U Lindberg.   

Abstract

Messenger ribonucleoprotein and mRNA from KB-cells were isolated under conditions designed to minimize nonspecific RNA-protein interaction and to minimize degradation by contaminating ribonucleases. A large fraction, 60-70%, of the messenger ribonucleoprotein from polysomes dissociated in vitro by either EDTA or puromycin sedimented faster than the large ribosome subunit. Messenger ribonucleoprotein particles with sedimentation coefficients up to 200 S were observed. Released mRNA was also large, with maximal molecular weights around 5 x 10(6).

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Year:  1972        PMID： 4501582      PMCID： PMC426534          DOI： 10.1073/pnas.69.3.681

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  27 in total

1.  Amino acid metabolism in mammalian cell cultures.

Authors:  H EAGLE
Journal:  Science       Date:  1959-08-21       Impact factor: 47.728

2.  SV40-specific RNA in the nucleus and polyribosomes of transformed cells.

Authors:  U Lindberg; J E Darnell
Journal:  Proc Natl Acad Sci U S A       Date:  1970-04       Impact factor: 11.205

3.  Localization and kinetics of formation of nuclear heterodisperse RNA, cytoplasmic heterodisperse RNA and polyribosome-associated messenger RNA in HeLa cells.

Authors:  S Penman; C Vesco; M Penman
Journal:  J Mol Biol       Date:  1968-05-28       Impact factor: 5.469

4.  Structure and function of mammalian ribosomes. I. Isolation and characterization of active liver ribosomal subunits.

Authors:  A K Falvey; T Staehelin
Journal:  J Mol Biol       Date:  1970-10-14       Impact factor: 5.469

5.  Messenger RNA in HeLa cells: an investigation of free and polyribosome-bound cytoplasmic messenger ribonucleoprotein particles by kinetic labelling and electron microscopy.

Authors:  G Spohr; N Granboulan; C Morel; K Scherrer
Journal:  Eur J Biochem       Date:  1970-12

6.  Release, identification, and isolation of messenger RNA from mammalian ribosomes.

Authors:  G Blobel
Journal:  Proc Natl Acad Sci U S A       Date:  1971-04       Impact factor: 11.205

7.  Messenger RNA-protein complexes and newly synthesized ribosomal subunits: analysis of free particles and components of polyribosomes.

Authors:  R P Perry; D E Kelley
Journal:  J Mol Biol       Date:  1968-07-14       Impact factor: 5.469

8.  Messenger RNA in rat liver polyribosomes: evidence that it exists as ribonucleoprotein particles.

Authors:  E C Henshaw
Journal:  J Mol Biol       Date:  1968-09-28       Impact factor: 5.469

9.  Isolation and biological activity of mammalian helical polyribosomes.

Authors:  A Monneron; C C Liew; V G Allfrey
Journal:  J Mol Biol       Date:  1971-04-28       Impact factor: 5.469

10.  Characterization of the messenger ribonucleoprotein released from reticulocyte polyribosomes by EDTA treatment.

Authors:  B Lebleu; G Marbaix; G Huez; J Temmerman; A Burny; H Chantrenne
Journal:  Eur J Biochem       Date:  1971-03-11
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  43 in total

1.  Adenovirus transcription. II. RNA sequences complementary to simian virus 40 and adenovirus 2DNA in AD2+ND1- and AD2+ND3-infected cells.

Authors:  S J Flint; Y Wewerka-Lutz; A S Levine; J Sambrook; P A Sharp
Journal:  J Virol       Date:  1975-09       Impact factor: 5.103

2.  Structure of transcriptionally-active chromatin subunits.

Authors:  J M Gottesfeld; P J Butler
Journal:  Nucleic Acids Res       Date:  1977-09       Impact factor: 16.971

3.  Deletion analysis of a defective interfering Semliki Forest virus RNA genome defines a region in the nsP2 sequence that is required for efficient packaging of the genome into virus particles.

Authors:  C L White; M Thomson; N J Dimmock
Journal:  J Virol       Date:  1998-05       Impact factor: 5.103

4.  Structure of germ line immunoglobulin alpha heavy-chain RNA and its location on polysomes.

Authors:  G Radcliffe; Y C Lin; M Julius; K B Marcu; J Stavnezer
Journal:  Mol Cell Biol       Date:  1990-01       Impact factor: 4.272

5.  Characterization of mRNA-protein complexes from mammalian cells.

Authors:  B Sundquist; T Persson; U Lindberg
Journal:  Nucleic Acids Res       Date:  1977-04       Impact factor: 16.971

6.  Effect of homopolyribonucleotides on messenger ribonucleoprotein particles.

Authors:  B Sundquist; T Persson
Journal:  Nucleic Acids Res       Date:  1977-04       Impact factor: 16.971

7.  Stimulation of estrogen receptor mRNA levels in MCF-7 cells by herpes simplex virus infection.

Authors:  E A Offord; R E Leake; J C Macnab
Journal:  J Virol       Date:  1989-05       Impact factor: 5.103

8.  Detailed analysis of the mRNAs mapping in the short unique region of herpes simplex virus type 1.

Authors:  F J Rixon; D J McGeoch
Journal:  Nucleic Acids Res       Date:  1985-02-11       Impact factor: 16.971

9.  trans activation of the latent Epstein-Barr virus (EBV) genome after transfection of the EBV DNA fragment.

Authors:  K Takada; N Shimizu; S Sakuma; Y Ono
Journal:  J Virol       Date:  1986-03       Impact factor: 5.103

10.  Adenovirus transcription. IV. Synthesis of viral-specific RNA in human cells infected with temperature-sensitive mutants of adenovirus 5.

Authors:  S M Berget; S J Flint; J F Williams; P A Sharp
Journal:  J Virol       Date:  1976-09       Impact factor: 5.103
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