Literature DB >> 440973

The use of exonuclease III for preparing single stranded DNA for use as a template in the chain terminator sequencing method.

A J Smith.   

Abstract

Exonuclease III, which degrades DNA 3' leads to 5' and is specific for duplex DNA, can be used to prepare single stranded DNA from linear duplexes. This is shown to be suitable as a template for use with the chain terminator DNA sequencing method of Sanger et al. [1]. Strategies are discussed for preparing single stranded DNA templates by this method and, in particular, its application to the sequence analysis of DNA cloned in plasmid vectors.

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Substances：

Year:  1979        PMID： 440973      PMCID： PMC327737          DOI： 10.1093/nar/6.3.831

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  9 in total

1.  A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. II. CHARACTERIZATION OF THE EXONUCLEASE ACTIVITY.

Authors:  C C RICHARDSON; I R LEHMAN; A KORNBERG
Journal:  J Biol Chem       Date:  1964-01       Impact factor: 5.157

2.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

3.  Direct determination of DNA nucleotide sequences: structure of a fragment of bacteriophage phiX172 DNA.

Authors:  F Galibert; J Sedat; E Ziff
Journal:  J Mol Biol       Date:  1974-08-15       Impact factor: 5.469

4.  Enzymatic removal and replacement of nucleotides at single strand breaks in deoxyribonucleic acid.

Authors:  Y Masamune; R A Fleischman; C C Richardson
Journal:  J Biol Chem       Date:  1971-04-25       Impact factor: 5.157

5.  Is there a correction mechanism in the 5S multigene system?

Authors:  J R Miller; G G Brownlee
Journal:  Nature       Date:  1978-10-12       Impact factor: 49.962

6.  Nucleotide sequence of bacteriophage G4 DNA.

Authors:  G N Godson; B G Barrell; R Staden; J C Fiddes
Journal:  Nature       Date:  1978-11-16       Impact factor: 49.962

7.  The use of thin acrylamide gels for DNA sequencing.

Authors:  F Sanger; A R Coulson
Journal:  FEBS Lett       Date:  1978-03-01       Impact factor: 4.124

8.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

9.  The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. I. The AT-rich spacer.

Authors:  N V Fedoroff; D D Brown
Journal:  Cell       Date:  1978-04       Impact factor: 41.582
  9 in total
  35 in total

1.  DNA microarrays with stem-loop DNA probes: preparation and applications.

Authors:  N E Broude; K Woodward; R Cavallo; C R Cantor; D Englert
Journal:  Nucleic Acids Res       Date:  2001-10-01       Impact factor: 16.971

2.  Tissue-specific and constitutive alpha-tubulin genes of Drosophila melanogaster code for structurally distinct proteins.

Authors:  W E Theurkauf; H Baum; J Bo; P C Wensink
Journal:  Proc Natl Acad Sci U S A       Date:  1986-11       Impact factor: 11.205

3.  Regions of the polyoma genome coding for T antigens.

Authors:  T Hunter; M A Hutchinson; W Eckhart; T Friedmann; A Esty; P LaPorte; P Deininger
Journal:  Nucleic Acids Res       Date:  1979-12-20       Impact factor: 16.971

4.  Genome organization and nucleotide sequence of human papillomavirus type 33, which is associated with cervical cancer.

Authors:  S T Cole; R E Streeck
Journal:  J Virol       Date:  1986-06       Impact factor: 5.103

5.  T5 Exonuclease Hydrolysis of Hepatitis B Virus Replicative Intermediates Allows Reliable Quantification and Fast Drug Efficacy Testing of Covalently Closed Circular DNA by PCR.

Authors:  Bingqian Qu; Yi Ni; Florian A Lempp; Florian W R Vondran; Stephan Urban
Journal:  J Virol       Date:  2018-11-12       Impact factor: 5.103

6.  A mammalian mitochondrial serine transfer RNA lacking the "dihydrouridine" loop and stem.

Authors:  M H de Bruijn; P H Schreier; I C Eperon; B G Barrell; E Y Chen; P W Armstrong; J F Wong; B A Roe
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971

7.  Isolation of a genomic clone partially encoding human hypoxanthine phosphoribosyltransferase.

Authors:  D J Jolly; A C Esty; H U Bernard; T Friedmann
Journal:  Proc Natl Acad Sci U S A       Date:  1982-08       Impact factor: 11.205

8.  Molecular cloning of human terminal deoxynucleotidyltransferase.

Authors:  R C Peterson; L C Cheung; R J Mattaliano; L M Chang; F J Bollum
Journal:  Proc Natl Acad Sci U S A       Date:  1984-07       Impact factor: 11.205

9.  Structure of genes for human growth hormone and chorionic somatomammotropin.

Authors:  J C Fiddes; P H Seeburg; F M DeNoto; R A Hallewell; J D Baxter; H M Goodman
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

10.  Common and distinct regions of defective-interfering RNAs of Sindbis virus.

Authors:  S S Monroe; S Schlesinger
Journal:  J Virol       Date:  1984-03       Impact factor: 5.103
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