Chromosome assignment of the T-antigen gene of simian virus 40 in African green monkey cells transformed by adeno 7-SV40 hybrid.

Somatic cell hybrids between mouse cells deficient in thymidine kinase [ATP:thymidine 5'-phosphotransferase (EC 2.7.1.75)] and two different monkey cell lines transformed by an adeno 7-SV40 hybrid have been produced using both a semiselective and a double selective procedure. Concordant segregation of the expression of SV40 T antigen with a specific monkey chromosome has been observed in all the mouse-monkey hybrid clones examined. Subcloning of three SV40 T antigen positive hybrid clones resulted in their segregation into SV40 T antigen-positive and negative subclones. Positive correlation between the SV40 T antigen and the same monkey chromosome has been observed in all the subclones examined.