Literature DB >> 4340167

Effect of environmental pH on the efficiency of cellular hybridization.

C M Croce, H Koprowski, H Eagle.   

Abstract

The hybridization of a human and mouse cell was strikingly pH-dependent, with a well defined optimum at (about) pH 7.6-8.0. The yield of hybrid cell colonies (1 per 500-2000 heterokaryocytes) was several hundred times greater than that obtained at pH 6.8-7.2. Although there was a significant effect on the efficiency of cell fusion, the critical time for the pH effect was in the first 4-8 days after fusion, presumably while viable hybrids were being formed from the multinucleated heterokaryocytes.

Entities:  

Mesh：

Substances：

Year:  1972        PMID： 4340167      PMCID： PMC426839          DOI： 10.1073/pnas.69.7.1953

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  12 in total

1.  Amino acid metabolism in mammalian cell cultures.

Authors:  H EAGLE
Journal:  Science       Date:  1959-08-21       Impact factor: 47.728

2.  pH as a determinant of cellular growth and contact inhibition.

Authors:  C Ceccarini; H Eagle
Journal:  Proc Natl Acad Sci U S A       Date:  1971-01       Impact factor: 11.205

3.  Induction of homokaryocyte, heterokaryocyte and hybrid formation by lysolecithin.

Authors:  C M Croce; W Sawicki; D Kritchevsky; H Koprowski
Journal:  Exp Cell Res       Date:  1971-08       Impact factor: 3.905

4.  A quantitative comparison of formation of spontaneous and virus-produced viable hybrids.

Authors:  H G Coon; M C Weiss
Journal:  Proc Natl Acad Sci U S A       Date:  1969-03       Impact factor: 11.205

5.  Loss of T-antigen from somatic hybrids between mouse cells and SV40-transformed human cells.

Authors:  M C Weiss; B Ephrussi; L J Scaletta
Journal:  Proc Natl Acad Sci U S A       Date:  1968-04       Impact factor: 11.205

6.  The use of drug-resistant markers to study the hybridization of mouse fibroblasts.

Authors:  J W Littlefield
Journal:  Exp Cell Res       Date:  1966-01       Impact factor: 3.905

7.  Buffer combinations for mammalian cell culture.

Authors:  H Eagle
Journal:  Science       Date:  1971-10-29       Impact factor: 47.728

8.  Preliminary analysis of the requirements for fusion from within and fusion from without by Newcastle disease virus.

Authors:  M A Bratt; W R Gallaher
Journal:  Proc Natl Acad Sci U S A       Date:  1969-10       Impact factor: 11.205

9.  Hydrogen ion buffers for biological research.

Authors:  N E Good; G D Winget; W Winter; T N Connolly; S Izawa; R M Singh
Journal:  Biochemistry       Date:  1966-02       Impact factor: 3.162

10.  Specific banding patterns of human chromosomes.

Authors:  M E Drets; M W Shaw
Journal:  Proc Natl Acad Sci U S A       Date:  1971-09       Impact factor: 11.205
View more
  31 in total

1.  Expression of human and suppression of mouse nucleolus organizer activity in mouse-human somatic cell hybrids.

Authors:  O J Miller; D A Miller; V G Dev; R Tantravahi; C M Croce
Journal:  Proc Natl Acad Sci U S A       Date:  1976-12       Impact factor: 11.205

2.  Somatic hybrid of thymus leukemia (.

Authors:  W Liang; E P Cohen
Journal:  Proc Natl Acad Sci U S A       Date:  1975-05       Impact factor: 11.205

3.  Assignment of gene(s) for cell transformation to human chromosome 7 carrying the simian virus 40 genome.

Authors:  C M Croce; H Koprowski
Journal:  Proc Natl Acad Sci U S A       Date:  1975-05       Impact factor: 11.205

4.  Repertoire of antiviral antibodies expressed by somatic cell hybrids.

Authors:  W Gerhard; C M Croce; D Lopes; H Koprowski
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

5.  Chimeric mice derived from human-mouse hybrid cells.

Authors:  K Illmensee; P C Hoppe; C M Croce
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

6.  Loss of mouse chromosomes in somatic cell hybrids between HT-1080 human fibrosarcoma cells and mouse peritioneal macrophages.

Authors:  C M Croce
Journal:  Proc Natl Acad Sci U S A       Date:  1976-09       Impact factor: 11.205

7.  Release by human chromosome 3 of the block at G1 of the cell cycle, in hybrids between tsAF8 hamster and human cells.

Authors:  P M Ming; H L Chang; R Baserga
Journal:  Proc Natl Acad Sci U S A       Date:  1976-06       Impact factor: 11.205

8.  Fusion of mammalian somatic cells by microsurgery.

Authors:  E G Diacumakos; E L Tatum
Journal:  Proc Natl Acad Sci U S A       Date:  1972-10       Impact factor: 11.205

9.  Selection of mouse X hamster hybrids using HAT medium and a polyene antibiotic.

Authors:  N I Goldstein; P B Fisher
Journal:  In Vitro       Date:  1978-02

10.  Segregation of rat chromosomes in somatic cell hybrids between rat cells and HT 1080 human fibrosarcoma cells.

Authors:  G G D'Ancona; C M Croce
Journal:  Hum Genet       Date:  1979       Impact factor: 4.132
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.