| Literature DB >> 4257981 |
J E Sjöström, M Lindberg, L Philipson.
Abstract
Staphylococcus aureus cells of strain 8325 (N) are competent for phage deoxyribonucleic acid (DNA) when harvested in the early exponential growth phase. Phenotypic expression of the competence requires divalent cations, and calcium ions are most effective. Treatment of phage DNA with deoxyribonuclease completely destroys infectivity and heat-denaturated DNA is not infectious. The highest frequency of transfection is around 10(4) plaque-forming units per mug of DNA.Entities:
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Year: 1972 PMID: 4257981 PMCID: PMC247278 DOI: 10.1128/jb.109.1.285-291.1972
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490