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Literature DB >> 7544

Calcium-requiring step in the uptake of deoxyribonucleic acid molecules through the surface of competent pneumococci.

Abstract

The conversion of surface-adsorbed deoxyribonucleic acid (DNA) molecules to a state in which they are inaccessible to exogenous deoxyribonuclease requires specifically calcium ions; magnesium ions cannot replace calcium ions. Virtually maximal levels of nuclease-resistant DNA binding and genetic transformation can be obtained in media free from magnesium and containing only calcium ions. It is suggested that the calcium-requiring process is the transport of DNA molecules across the plasma membrane. Magnesium ions stimulate both the loss of surface-adsorbed DNA to the medium and the extracellular degradation of DNA.

Entities: Chemical

Mesh：

Substances：

Year: 1976 PMID： 7544 PMCID： PMC233133 DOI： 10.1128/jb.126.3.1113-1118.1976

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

18 in total

1. Initiation of bacterial transformation.

Authors: M S FOX; R D HOTCHKISS
Journal: Nature Date: 1957-06-29 Impact factor: 49.962

2. Identification of a deoxyribonuclease implicated in genetic transformation of Diplococcus pneumoniae.

Authors: S Lacks; B Greenberg; M Neuberger
Journal: J Bacteriol Date: 1975-07 Impact factor: 3.490

3. Nucleolytic degradation of homologous and heterologous deoxyribonucleic acid molecules at the surface of competent pneumococci.

Authors: H Seto; R Lopez; O Garrigan; A Tomasz
Journal: J Bacteriol Date: 1975-05 Impact factor: 3.490

4. Cell surface-located deoxyribonucleic acid receptors in transformable pneumococci.

Authors: H Seto; R Lopez; A Tomasz
Journal: J Bacteriol Date: 1975-06 Impact factor: 3.490

5. Sensitivity of Escherichia coli to viral nucleic acid. 8. Idiosyncrasy of Ca2+-dependent competence for DNA.

Authors: A Taketo
Journal: J Biochem Date: 1974-04 Impact factor: 3.387

6. Competence for deoxyribonucleic acid uptake and deoxyribonuclease action external to cells in the genetic transformation of Diplococcus pneumoniae.

Authors: S Lacks; B Greenberg
Journal: J Bacteriol Date: 1973-04 Impact factor: 3.490

3. Interactions of competent Streptococcus sanguis (Wicky) cells with native or denatured, homologous or heterologous deoxyribonucleic acids.

Authors: J M Ranhand
Journal: J Bacteriol Date: 1980-05 Impact factor: 3.490

4. Recovery of competence in calcium-limited Azotobacter vinelandii.

Authors: W J Page; J L Doran
Journal: J Bacteriol Date: 1981-04 Impact factor: 3.490

5. Mutations which alter the kinetics of calcium transport alter the regulation of competence in Streptococcus pneumoniae.

Authors: M C Trombe; V Rieux; F Baille
Journal: J Bacteriol Date: 1994-04 Impact factor: 3.490

6. Transformation in Bacillus subtilis: biological and physical evidence for a novel DNA-intermediate in synchronously transforming cells.

Authors: J Buitenwerf; G Venema
Journal: Mol Gen Genet Date: 1977-11-14

7. Effect of ethylenediaminetetraacetic acid on deoxyribonucleic acid entry and recombination in transformation of a wild-type strain and a rec-1 mutant of Haemophilus influenzae.

Authors: M Noteborn; G Venema; J Kooistra
Journal: J Bacteriol Date: 1981-03 Impact factor: 3.490

Calcium-requiring step in the uptake of deoxyribonucleic acid molecules through the surface of competent pneumococci.

1. Initiation of bacterial transformation.

2. Identification of a deoxyribonuclease implicated in genetic transformation of Diplococcus pneumoniae.

3. Nucleolytic degradation of homologous and heterologous deoxyribonucleic acid molecules at the surface of competent pneumococci.

4. Cell surface-located deoxyribonucleic acid receptors in transformable pneumococci.

5. Sensitivity of Escherichia coli to viral nucleic acid. 8. Idiosyncrasy of Ca2+-dependent competence for DNA.

6. Competence for deoxyribonucleic acid uptake and deoxyribonuclease action external to cells in the genetic transformation of Diplococcus pneumoniae.

7. Breakage prior to entry of donor DNA in Pneumococcus transformation.

8. Structure of deoxyribonucleic acid on the cell surface during uptake by pneumococcus.

9. Early stages in DNA binding and uptake during genetic transformation of pneumococci.

10. Role of a deoxyribonuclease in the genetic transformation of Diplococcus pneumoniae.

1. Inhibitors of genetic recombination in pneumococci.

2. Early stages in Bacillus subtilis transformation: association between homologous DNA and surface structures.

3. Interactions of competent Streptococcus sanguis (Wicky) cells with native or denatured, homologous or heterologous deoxyribonucleic acids.

4. Recovery of competence in calcium-limited Azotobacter vinelandii.

5. Mutations which alter the kinetics of calcium transport alter the regulation of competence in Streptococcus pneumoniae.

6. Transformation in Bacillus subtilis: biological and physical evidence for a novel DNA-intermediate in synchronously transforming cells.

7. Effect of ethylenediaminetetraacetic acid on deoxyribonucleic acid entry and recombination in transformation of a wild-type strain and a rec-1 mutant of Haemophilus influenzae.

8. Consequences of Ca2+ deficiency on macromolecular synthesis and adenylate energy charge in Yersinia pestis.

9. Protease-sensitive transfection of Streptococcus pneumoniae with bacteriophage Cp-1 DNA.

10. Uptake of plasmid deoxyribonucleic acid by Haemophilus.