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Literature DB >> 14066414

INCORPORATION OF DEOXYRIBONUCLEIC ACID IN THE BACILLUS SUBTILIS TRANSFORMATION SYSTEM.

Abstract

Young, F. E. (Western Reserve University Cleveland, Ohio) and John Spizizen. Incorporation of deoxyribonucleic acid in the Bacillus subtilis transformation system. J. Bacteriol. 86:392-400. 1963.-The optimal conditions for the incorporation of deoxyribonucleic acid (DNA) were studied. In competent cells, the irreversible binding of DNA was influenced by temperature, hydrogen ion concentration, and aeration. Divalent cations, such as barium, strontium, calcium, or magnesium, were required. Under suboptimal environmental conditions and with metabolic inhibitors, the process of transformation was decreased to a greater extent than was incorporation of DNA. Under conditions of phosphate depletion, the incorporation of P(32) increased. However, the frequency of transformation decreased. This inducible process was not related to competence.

Entities: Chemical Disease Species

Keywords: BACILLUS SUBTILIS; DNA, BACTERIAL; EXPERIMENTAL LAB STUDY

Mesh：

Substances：

Year: 1963 PMID： 14066414 PMCID： PMC278448 DOI： 10.1128/jb.86.3.392-400.1963

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

22 in total

6. DEVELOPMENT OF COMPETENCE FOR TRANSFORMATION EXPERIMENTS IN AN OVERNIGHT CULTURE OF GERMINATING SPORES OF BACILLUS SUBTILIS.

Authors: E EPHRATI-ELIZUR
Journal: J Bacteriol Date: 1965-08 Impact factor: 3.490

7. Natural competence for DNA transformation by Legionella pneumophila and its association with expression of type IV pili.

Authors: B J Stone; Y A Kwaik
Journal: J Bacteriol Date: 1999-03 Impact factor: 3.490

INCORPORATION OF DEOXYRIBONUCLEIC ACID IN THE BACILLUS SUBTILIS TRANSFORMATION SYSTEM.

1. Emergence of competence (for transformation) of three Hemophilus species in a chemically defined environment.

2. Transformability of Haemophilus influenzae.

3. The fate of transforming deoxyribonucleate following fixation by transformable bacteria. III.

4. Physiological and genetic factors affecting transformation of Bacillus subtilis.

5. Fate of transforming deoxyribonucleate following fixation by transformable bacteria.

6. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.

7. STRAND SEPARATION AND SPECIFIC RECOMBINATION IN DEOXYRIBONUCLEIC ACIDS: BIOLOGICAL STUDIES.

8. ON THE SIZE OF GENETIC DETERMINANTS IN PNEUMOCOCCUS AND THE NATURE OF THE VARIABLES INVOLVED IN TRANSFORMATION.

9. THE DEPENDENCE OF PNEUMOCOCCAL TRANSFORMATION ON THE MOLECULAR WEIGHT OF DEOXYRIBOSE NUCLEIC ACID.

10. Studies on transformations of Hemophilus influenzae. I. Competence.

1. Physiological factors affecting transformation of Azotobacter vinelandii.

2. Effect of pH on competence development and deoxyribonucleic acid uptake in Streptococcus sanguis (Wicky).

3. Factors affecting genetic transformation of Neisseria gonorrhoeae.

4. Genetic exchange in Bacillus subtilis in soil.

5. ANTIBIOTIC GLYCOSIDES. IV. STUDIES ON THE MECHANISM OF ERYTHROMYCIN RESISTANCE IN BACILLUS SUBTILIS.

6. DEVELOPMENT OF COMPETENCE FOR TRANSFORMATION EXPERIMENTS IN AN OVERNIGHT CULTURE OF GERMINATING SPORES OF BACILLUS SUBTILIS.

7. Natural competence for DNA transformation by Legionella pneumophila and its association with expression of type IV pili.

8. Non-transformable mutants of Bacillus subtilis defective in the penetration of DNA into the cell.

9. Purification and properties of a manganese-stimulated endonuclease from Bacillus subtilis.

10. Factors affecting transfection in Bacillus stearothermophilus.