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Literature DB >> 4247491

Rapid purification and the immunological specificity of mammalian microtubular paracrystals possessing an ATPase activity.

Abstract

A new procedure for the rapid purification of vinblastine-induced microtubular paracrystals is described. The paracrystals constitute about 2 per cent of the total protein in L cells. They contain a labile ATPase with a Michaelis constant of 1.0 x 10(-4). In the immunofluorescence test, sera prepared against paracrystals reacted positively with intracellular paracrystals and the mitotic apparatus. Antisera against paracrystals from a malignant murine cell also labeled the mitotic apparatus of HeLa and chicken embryonic cells, revealing the presence of a common or related antigen in the mitotic apparatus of these higher vertebrates.

Entities: Chemical Gene Species

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Year: 1970 PMID： 4247491 PMCID： PMC283068 DOI： 10.1073/pnas.66.2.464

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

14 in total

1. STUDIES ON THE PROTEIN COMPONENTS OF CILIA FROM TETRAHYMENA PYRIFORMIS.

Authors: I R GIBBONS
Journal: Proc Natl Acad Sci U S A Date: 1963-11 Impact factor: 11.205

2. ASSOCIATION BETWEEN THE SPINDLE APPARATUS AND REOVIRUS.

Authors: S DALES
Journal: Proc Natl Acad Sci U S A Date: 1963-08 Impact factor: 11.205

3. A negative staining method for high resolution electron microscopy of viruses.

Authors: S BRENNER; R W HORNE
Journal: Biochim Biophys Acta Date: 1959-07

4. Nutrition needs of mammalian cells in tissue culture.

Authors: H EAGLE
Journal: Science Date: 1955-09-16 Impact factor: 47.728

5. Fluorescent antibody studies with agents of varicella and herpes zoster propagated in vitro.

Authors: T H WELLER; A H COONS
Journal: Proc Soc Exp Biol Med Date: 1954 Aug-Sep

6. Localization of nucleotide phosphohydrolase activity within vaccinia.

Authors: P H Gold; S Dales
Journal: Proc Natl Acad Sci U S A Date: 1968-07 Impact factor: 11.205

7. Divalent cation activation of flagellar ATP-phosphohydrolase from bull sperm.

Authors: L G Young; L Nelson
Journal: J Cell Physiol Date: 1969-12 Impact factor: 6.384

8. Microtubule crystals: a new biophysical phenomenon induced by Vinca alkaloids.

Authors: K G Bensch; S E Malawista
Journal: Nature Date: 1968-06-22 Impact factor: 49.962

9. The colchicine-binding protein of mammalian brain and its relation to microtubules.

Authors: R C Weisenberg; G G Borisy; E W Taylor
Journal: Biochemistry Date: 1968-12 Impact factor: 3.162

10. Isolation of microtubule protein from cultured mouse neuroblastoma cells.

Authors: J B Olmsted; K Carlson; R Klebe; F Ruddle; J Rosenbaum
Journal: Proc Natl Acad Sci U S A Date: 1970-01 Impact factor: 11.205

22 in total

1. Cytochemical approaches to the localization of specific adenosine triphosphatases.

Authors: J A Firth
Journal: Histochem J Date: 1978-05

2. Tubulin aggregation and disaggregation: mediation by two distinct vinblastine-binding sites.

Authors: B Bhattacharyya; J Wolff
Journal: Proc Natl Acad Sci U S A Date: 1976-07 Impact factor: 11.205

3. Adenosine triphosphatases of rat pancreatic islets: comparison with those of rat kidney.

Authors: S R Levin; B G Kasson; J F Driessen
Journal: J Clin Invest Date: 1978-09 Impact factor: 14.808

4. Adenosine triphosphatase activity in the neural lobe of the bovine pituitary gland.

Authors: H Vilhardt; D B Hope
Journal: Biochem J Date: 1974-10 Impact factor: 3.857

5. Isolation and partial characterization of alpha and beta-tubulin from outer doublets of sea-urchin sperm and microtubules of chick-embryo brain.

Authors: R F Luduena; D O Woodward
Journal: Proc Natl Acad Sci U S A Date: 1973-12 Impact factor: 11.205

6. Antibody against tuberlin: the specific visualization of cytoplasmic microtubules in tissue culture cells.

Authors: K Weber; R Pollack; T Bibring
Journal: Proc Natl Acad Sci U S A Date: 1975-02 Impact factor: 11.205

7. Immunocytochemistry of the Microtubules of fat-laden cells. Brown fat cells and adrenocortical cells in primary monolayer culture.

Authors: H Sugihara; N Yonemitsu; K Ohta; S Miyabara; A Nagayama
Journal: Histochemistry Date: 1983

8. A fine structural study of the formation of temporary swellings (Herring bodies) and reversible degeneration of neurosecretory axons following microiontophoretic ejection of vinblastine into the hypothalamo-neurohypophysial tract of the frog (Rana pipiens).

Authors: A C Chang; H D Dellmann
Journal: Exp Brain Res Date: 1984 Impact factor: 1.972

9. Isolation and characterization of cytoplasmic inclusions from influenza A virus-infected cells.

Authors: M W Shaw; R W Compans
Journal: J Virol Date: 1978-02 Impact factor: 5.103

10. The pathogenesis of epizootic hemorrhagic disease of deer: an electron microscopic study.

Authors: K Tsai; L Karstad
Journal: Am J Pathol Date: 1973-03 Impact factor: 4.307