Literature DB >> 5263744

Isolation of microtubule protein from cultured mouse neuroblastoma cells.

J B Olmsted, K Carlson, R Klebe, F Ruddle, J Rosenbaum.   

Abstract

The addition of vinblastine to high-speed supernatants derived from homogenates of cultured mouse neuroblastoma cells results in the formation of a precipitate which has been characterized as microtubule protein by the following criteria: colchicine-binding activity, molecular weight, amino acid composition, and electrophoretic mobility. The method therefore permits the rapid isolation of microtubule protein from crude supernatants of neuroblastoma cells.

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Year:  1970        PMID: 5263744      PMCID: PMC286201          DOI: 10.1073/pnas.65.1.129

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN: 0027-8424            Impact factor:   11.205


  15 in total

1.  Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.

Authors:  A L Shapiro; E Viñuela; J V Maizel
Journal:  Biochem Biophys Res Commun       Date:  1967-09-07       Impact factor: 3.575

2.  Neuronal changes induced by intrathecal vincristine sulfate.

Authors:  S S Schochet; P W Lampert; K M Earle
Journal:  J Neuropathol Exp Neurol       Date:  1968-10       Impact factor: 3.685

3.  On the structural protein of flagellar outer fibers.

Authors:  R E Stephens
Journal:  J Mol Biol       Date:  1968-03-14       Impact factor: 5.469

4.  A high-resolution method for slicing and counting radioactivity in polyacrylamide gels.

Authors:  R H Gray; D M Steffensen
Journal:  Anal Biochem       Date:  1968-07       Impact factor: 3.365

5.  The binding of colchicine by sarcoma 180 cells.

Authors:  W A Creasey; T C Chou
Journal:  Biochem Pharmacol       Date:  1968-03       Impact factor: 5.858

6.  Procedure for embedding in situ selected cells cultured in vitro.

Authors:  B R Brinkley; P Murphy; L C Richardson
Journal:  J Cell Biol       Date:  1967-10       Impact factor: 10.539

7.  Effects of mitotic spindle inhibitors on neurotubules and neurofilaments in anterior horn cells.

Authors:  H Wisniewski; M L Shelanski; R D Terry
Journal:  J Cell Biol       Date:  1968-07       Impact factor: 10.539

8.  Microtubular crystals in mammalian cells.

Authors:  K G Bensch; S E Malawista
Journal:  J Cell Biol       Date:  1969-01       Impact factor: 10.539

9.  Properties of the protein subunit of central-pair and outer-doublet microtubules of sea urchin flagella.

Authors:  M L Shelanski; E W Taylor
Journal:  J Cell Biol       Date:  1968-08       Impact factor: 10.539

10.  The mechanism of action of colchicine. Binding of colchincine-3H to cellular protein.

Authors:  G G Borisy; E W Taylor
Journal:  J Cell Biol       Date:  1967-08       Impact factor: 10.539

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  91 in total

1.  Human, but not bovine, oxidized cerebral spinal fluid lipoproteins disrupt neuronal microtubules.

Authors:  M D Neely; L L Swift; T J Montine
Journal:  Lipids       Date:  2000-11       Impact factor: 1.880

2.  The role of microtubules in the assembly of vinblastine-induced crystals.

Authors:  H Stebbings
Journal:  Cell Tissue Res       Date:  1975-05-27       Impact factor: 5.249

3.  Gene expression in mouse neuroblastoma cells: properties of the genome.

Authors:  M S Zornetzer; G S Stein
Journal:  Proc Natl Acad Sci U S A       Date:  1975-08       Impact factor: 11.205

4.  The armadillo repeat-containing protein, ARMCX3, physically and functionally interacts with the developmental regulatory factor Sox10.

Authors:  Zhongming Mou; Andrew R Tapper; Paul D Gardner
Journal:  J Biol Chem       Date:  2009-03-20       Impact factor: 5.157

5.  Structure-based inhibitors of amyloid beta core suggest a common interface with tau.

Authors:  Sarah L Griner; Paul Seidler; Jeannette Bowler; Kevin A Murray; Tianxiao Peter Yang; Shruti Sahay; Michael R Sawaya; Duilio Cascio; Jose A Rodriguez; Stephan Philipp; Justyna Sosna; Charles G Glabe; Tamir Gonen; David S Eisenberg
Journal:  Elife       Date:  2019-10-15       Impact factor: 8.140

6.  Dibutyryladenosine 3':5'-cyclic monophosphate-mediated changes in rat cells involve macromolecular alterations in vinblastine-precipitable proteins.

Authors:  A T Meza; M Rieber
Journal:  Biochem J       Date:  1978-09-15       Impact factor: 3.857

7.  A genomic toolkit to investigate kinesin and myosin motor function in cells.

Authors:  Zoltan Maliga; Magno Junqueira; Yusuke Toyoda; Andreas Ettinger; Felipe Mora-Bermúdez; Robin W Klemm; Andrej Vasilj; Elaine Guhr; Itziar Ibarlucea-Benitez; Ina Poser; Ezio Bonifacio; Wieland B Huttner; Andrej Shevchenko; Anthony A Hyman
Journal:  Nat Cell Biol       Date:  2013-02-17       Impact factor: 28.824

8.  CD40 is expressed and functional on neuronal cells.

Authors:  Jun Tan; Terrence Town; Takashi Mori; Demian Obregon; Yajuan Wu; Anthony DelleDonne; Amyn Rojiani; Fiona Crawford; Richard A Flavell; Mike Mullan
Journal:  EMBO J       Date:  2002-02-15       Impact factor: 11.598

Review 9.  In Vitro and In Vivo Modeling of Spinal and Bulbar Muscular Atrophy.

Authors:  Maria Pennuto; Manuela Basso
Journal:  J Mol Neurosci       Date:  2015-11-27       Impact factor: 3.444

10.  Differential in vitro action of S-12363, a new vinblastine derivative, and of its epimer on microtubule proteins.

Authors:  M Wright; M Garès; P Verdier-Pinard; A Moisand; M Berlion; J J Legrand; J P Bizzari
Journal:  Cancer Chemother Pharmacol       Date:  1991       Impact factor: 3.333

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