Literature DB >> 804694

Antibody against tuberlin: the specific visualization of cytoplasmic microtubules in tissue culture cells.

K Weber, R Pollack, T Bibring.   

Abstract

Cytoplasmic microtubules in tissue culture cells can be directly visualized by immunofluorescence microscopy. Antibody against tubulin from the outer doublets of sea urchin sperm flagella decorates a network of fine cytoplasmic fibers in a variety of cell lines of human, monkey, rat, mouse, and chicken origin. These fibers are separate and of uniform thickness and are seen throughout the cytoplasm. The fibers disappear either in a medium containing colchicine or after subjection of the cells to low temperature. The same treatments do not destroy the microfilamentous structures that are visualized by means of antibody against actin. When tryspin-treated enucleated cells are replated and then stained with antibody against tubulin, the fibers can be seen to traverse the entire enucleated cytoplasm.

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Year:  1975        PMID： 804694      PMCID： PMC432331          DOI： 10.1073/pnas.72.2.459

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  20 in total

1.  PURIFICATION OF HUMAN SERUM GAMMA-GLOBULIN FOR IMMUNOLOGIC STUDIES: GAMMA-GLOBULIN FRAGMENTATION AFTER SULFATE PRECIPITATION AND PROLONGED DIALYSIS.

Authors:  A J STRAUSS; P G KEMP; W E VANNIER; H C GOODMAN
Journal:  J Immunol       Date:  1964-07       Impact factor: 5.422

2.  Vinblastine and microtubules. II. Characterization of two protein subunits from the isolated crystals.

Authors:  J Bryan
Journal:  J Mol Biol       Date:  1972-04-28       Impact factor: 5.469

Review 3.  Actin and myosin and cell movement.

Authors:  T D Pollard; R R Weihing
Journal:  CRC Crit Rev Biochem       Date:  1974-01

4.  Rapid purification and the immunological specificity of mammalian microtubular paracrystals possessing an ATPase activity.

Authors:  A Nagayama; S Dales
Journal:  Proc Natl Acad Sci U S A       Date:  1970-06       Impact factor: 11.205

5.  Microtubule assembly in the absence of added nucleotides.

Authors:  M L Shelanski; F Gaskin; C R Cantor
Journal:  Proc Natl Acad Sci U S A       Date:  1973-03       Impact factor: 11.205

6.  Actin antibody: the specific visualization of actin filaments in non-muscle cells.

Authors:  E Lazarides; K Weber
Journal:  Proc Natl Acad Sci U S A       Date:  1974-06       Impact factor: 11.205

7.  Evidence for four classes of microtubules in individual cells.

Authors:  O Behnke; A Forer
Journal:  J Cell Sci       Date:  1967-06       Impact factor: 5.285

8.  Serological similarity of flagellar and mitotic microtubules.

Authors:  C Fulton; R E Kane; R E Stephens
Journal:  J Cell Biol       Date:  1971-09       Impact factor: 10.539

9.  Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.

Authors:  G J TODARO; H GREEN
Journal:  J Cell Biol       Date:  1963-05       Impact factor: 10.539

10.  Concerning the universality of a microtubule antigen in animal cells.

Authors:  S Dales
Journal:  J Cell Biol       Date:  1972-03       Impact factor: 10.539
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  66 in total

1.  Lateral transport on cell membranes: mobility of concanavalin A receptors on myoblasts.

Authors:  J Schlessinger; D E Koppel; D Axelrod; K Jacobson; W W Webb; E L Elson
Journal:  Proc Natl Acad Sci U S A       Date:  1976-07       Impact factor: 11.205

2.  Cytoplasmic microtubules in tissue culture cells appear to grow from an organizing structure towards the plasma membrane.

Authors:  M Osborn; K Weber
Journal:  Proc Natl Acad Sci U S A       Date:  1976-03       Impact factor: 11.205

3.  A protein factor essential for microtubule assembly.

Authors:  M D Weingarten; A H Lockwood; S Y Hwo; M W Kirschner
Journal:  Proc Natl Acad Sci U S A       Date:  1975-05       Impact factor: 11.205

4.  Extraction and immunochemical assays of a tubulin-like factor in cotton seedlings.

Authors:  A Rikin; D Atsmon; C Gitler
Journal:  Planta       Date:  1982-09       Impact factor: 4.116

5.  Microtubule inhibitors block the morphological changes induced in Drosophila blood cells by a parasitoid wasp factor.

Authors:  R M Rizki; T M Rizki
Journal:  Experientia       Date:  1990-03-15

6.  Distinction between smooth muscle, fibroblasts and endothelial cells in culture by the use of fluoresceinated antibodies against smooth muscle actin.

Authors:  J H Chamley; U Gröschel-Stewart; G R Campbell; G Burnstock
Journal:  Cell Tissue Res       Date:  1977-02-14       Impact factor: 5.249

7.  Antitubulin agents enhance the stimulation of DNA synthesis by polypeptide growth factors in 3T3 mouse fibroblasts.

Authors:  M Friedkin; A Legg; E Rozengurt
Journal:  Proc Natl Acad Sci U S A       Date:  1979-08       Impact factor: 11.205

8.  Studies on intracellular transport of secretory proteins in the rat exocrine pancreas. II. Inhibition of antimicrotubular agents.

Authors:  J Seybold; W Bieger; H F Kern
Journal:  Virchows Arch A Pathol Anat Histol       Date:  1975-11-28

9.  Phalloidin-induced actin polymerization in the cytoplasm of cultured cells interferes with cell locomotion and growth.

Authors:  J Wehland; M Osborn; K Weber
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  Organization and energy-dependent growth of microtubules in cells.

Authors:  F R Frankel
Journal:  Proc Natl Acad Sci U S A       Date:  1976-08       Impact factor: 11.205
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