Literature DB >> 424301

A new method for 3'-labelling of polyribonucleotides by phosphorylation with RNA ligase and its application to the 3'-modification for joining reactions.

E Ohtsuka, H Uemura, T Doi, T Miyake, S Nishikawa, M Ikehara.   

Abstract

P1-Adenosine 5'-P2-o-nitrobenzyl pyrophosphate (nbzlppA) has been synthesized as a substrate for T4 RNA ligase catalyzed 3'-phosphorylation. Incubation of oligoribonucleotides and nbzlppA with RNA ligase yielded oligoribonucleotides having a 3'-o-(o-nitrobenzyl) phosphate. Photochemical removal of the o-nitrobenzyl group provided the free 3'-phosphate. Using [P2-32P] nbzlppA, 3'-termini of oligoribonucleotides could be labelled with 32P. This reaction was applied to modify the 3'-end of donor molecules in joining reaction with RNA ligase. A trinucleotide U-A-G was converted to U-A-Gpnbzl and phosphorylated with polynucleotide kinase. pU-A-Gpnbzl was then joined to an acceptor trinucleotide A-U-G to yield A-U-G-U-A-Gp.

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Year:  1979        PMID： 424301      PMCID： PMC327706          DOI： 10.1093/nar/6.2.443

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  16 in total

1.  Equimolar addition of oligoribonucleotides with T4 RNA ligase.

Authors:  O C Uhlenbeck; V Cameron
Journal:  Nucleic Acids Res       Date:  1977-01       Impact factor: 16.971

2.  Dinucleoside pyrophosphate are substrates for T4-induced RNA ligase.

Authors:  T E England; R I Gumport; O C Uhlenbeck
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

3.  Polynucleotide kinase from a T4 mutant which lacks the 3' phosphatase activity.

Authors:  V Cameron; D Soltis; O C Uhlenbeck
Journal:  Nucleic Acids Res       Date:  1978-03       Impact factor: 16.971

4.  T4 RNA ligase: substrate chain length requirements.

Authors:  G Kaufmann; T Klein; U Z Littauer
Journal:  FEBS Lett       Date:  1974-09-15       Impact factor: 4.124

5.  Studies on ribonucleic acid ligase. Characterization of an adenosine triphosphate-inorganic pyrophosphate exchange reaction and demonstration of an enzyme-adenylate complex with T4 bacteriophage-induced enzyme.

Authors:  J W Cranston; R Silber; V G Malathi; J Hurwitz
Journal:  J Biol Chem       Date:  1974-12-10       Impact factor: 5.157

6.  Enzymatic oligoribonucleotide synthesis with T4 RNA ligase.

Authors:  T E England; O C Uhlenbeck
Journal:  Biochemistry       Date:  1978-05-30       Impact factor: 3.162

7.  The use of terminal blocking groups for the specific joining of oligonucleotides in RNA ligase reactions containing equimolar concentrations of acceptor and donor molecules.

Authors:  J J Sninsky; J A Last; P T Gilham
Journal:  Nucleic Acids Res       Date:  1976-11       Impact factor: 16.971

8.  Bacteriophage T4 RNA ligase. Reaction intermediates and interaction of substrates.

Authors:  A Sugino; T J Snoper; N R Cozzarelli
Journal:  J Biol Chem       Date:  1977-03-10       Impact factor: 5.157

9.  Joining of synthetic ribotrinucleotides with defined sequences catalyzed by T4 RNA ligase.

Authors:  E Ohtsuka; S Nishikawa; R Fukumoto; S Tanaka; A F Markham
Journal:  Eur J Biochem       Date:  1977-12-01

10.  Addition of mononucleotides to oligoribonucleotide acceptors with T4 RNA ligase.

Authors:  Y Kikuchi; F Hishinuma; K Sakaguchi
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205
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  8 in total

1.  Molecular design of a eukaryotic messenger RNA and its chemical synthesis.

Authors:  R Iwase; M Maeda; T Fujiwara; M Sekine; T Hata; K Miura
Journal:  Nucleic Acids Res       Date:  1992-04-11       Impact factor: 16.971

2.  Biotin and fluorescent labeling of RNA using T4 RNA ligase.

Authors:  R W Richardson; R I Gumport
Journal:  Nucleic Acids Res       Date:  1983-09-24       Impact factor: 16.971

3.  Preparation of oligonucleotides corresponding to the acceptor stem of yeast tRNAPhe and their interaction with yeast ATP(CTP):tRNA nucleotidyltransferase.

Authors:  G H Wang; L W McLaughlin; H Sternbach; F Cramer
Journal:  Nucleic Acids Res       Date:  1984-09-11       Impact factor: 16.971

4.  Replacement and insertion of nucleotides at the anticodon loop of E. coli tRNAMetf by ligation of chemically synthesized ribooligonucleotides.

Authors:  T Doi; A Yamane; J Matsugi; E Ohtsuka; M Ikehara
Journal:  Nucleic Acids Res       Date:  1985-05-24       Impact factor: 16.971

5.  Elongation of oligonucleotides in the 3'-direction with activated mononucleotides and their analogs using RNA ligase.

Authors:  E Ohtsuka; T Miyake; K Nagao; H Uemura; S Nishikawa; M Sugiura; M Ikehara
Journal:  Nucleic Acids Res       Date:  1980-02-11       Impact factor: 16.971

6.  Synthesis and reactivity of intermediates formed in the T4 RNA ligase reaction.

Authors:  P U Hoffmann; L W McLaughlin
Journal:  Nucleic Acids Res       Date:  1987-07-10       Impact factor: 16.971

7.  Modification of the anticodon triplet of E.coli tRNAMetf by replacement with trimers complementary to non-sense codons UAG and UAA.

Authors:  E Ohtsuka; T Doi; R Fukumoto; J Matsugi; M Ikehara
Journal:  Nucleic Acids Res       Date:  1983-06-25       Impact factor: 16.971

8.  Total synthesis of a RNA molecule with sequence identical to that of Escherichia coli formylmethionine tRNA.

Authors:  E Ohtsuka; S Tanaka; T Tanaka; T Miyake; A F Markham; E Nakagawa; T Wakabayashi; Y Taniyama; S Nishikawa; R Fukumoto; H Uemura; T Doi; T Tokunaga; M Ikehara
Journal:  Proc Natl Acad Sci U S A       Date:  1981-09       Impact factor: 11.205
  8 in total

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