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Literature DB >> 4197093

Studies on in vitro DNA synthesis. Purification of the dna G gene product from Escherichia coli.

Abstract

varphiX174 DNA-dependent dNMP incorporation is temperature-sensitive (ts) in extracts of uninfected E. coli dna A, B, C, D, E, and G ts strains. DNA synthesis can be restored in heat-inactivated extracts of various dna ts mutants by addition of extracts of wild-type or other dna ts mutants. A protein that restores activity to heat-inactivated extracts of dna G ts cells has been extensively purified. This protein has also been purified from dna G ts cells and is thermolabile when compared to the wild-type protein. The purified dna G protein has a molecular weight of about 60,000, is insensitive to N-ethylmaleimide, and binds poorly to DNA. It does not stimulate heat-inactivated crude extracts of dna B, C, D, or E ts cells and lacks detectable RNA and DNA polymerase activities.

Entities: Chemical Species

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Year: 1973 PMID： 4197093 PMCID： PMC433553 DOI： 10.1073/pnas.70.5.1613

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

20 in total

1. Deoxyribonucleic acid plymerase II. of Escherichia coli. I. The purification and characterization of the enzyme.

Authors: R B Wickner; B Ginsberg; I Berkower; J Hurwitz
Journal: J Biol Chem Date: 1972-01-25 Impact factor: 5.157

2. Escherichia coli mutants with temperature-sensitive synthesis of DNA.

Authors: P L Carl
Journal: Mol Gen Genet Date: 1970

3. Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division.

Authors: Y Hirota; A Ryter; F Jacob
Journal: Cold Spring Harb Symp Quant Biol Date: 1968

4. Fate of parental phi X174-DNA upon infection of starved thymine-requiring host cells.

Authors: B Francke; D S Ray
Journal: Virology Date: 1971-04 Impact factor: 3.616

5. Function of DNA polymerase 3 in DNA replication.

Authors: V Nüsslein; B Otto; F Bonhoeffer; H Schaller
Journal: Nat New Biol Date: 1971-12-29

6. Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.

Authors: M L Gefter; Y Hirota; T Kornberg; J A Wechsler; C Barnoux
Journal: Proc Natl Acad Sci U S A Date: 1971-12 Impact factor: 11.205

7. Replication and repair of DNA in cells of Escherichia coli treated with toluene.

Authors: R E Moses; C C Richardson
Journal: Proc Natl Acad Sci U S A Date: 1970-10 Impact factor: 11.205

8. Escherichia coli mutants temperature-sensitive for DNA synthesis.

Authors: J A Wechsler; J D Gross
Journal: Mol Gen Genet Date: 1971

9. Studies on the binding of RNA polymerase to polynucleotides.

Authors: O W Jones; P Berg
Journal: J Mol Biol Date: 1966-12-28 Impact factor: 5.469

10. RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form.

Authors: W Wickner; D Brutlag; R Schekman; A Kornberg
Journal: Proc Natl Acad Sci U S A Date: 1972-04 Impact factor: 11.205

24 in total

1. Genetic map location of the Escherichia coli dnaG gene.

Authors: P L Chen; P L Carl
Journal: J Bacteriol Date: 1975-12 Impact factor: 3.490

2. Partial suppression of the phenotype of Escherichia coli K-12 dnaG mutants by some I-like conjugative plasmids.

Authors: B M Wilkins
Journal: J Bacteriol Date: 1975-06 Impact factor: 3.490

3. Association of phiX174 DNA-dependent ATPase activity with an Escherichia coli protein, replication factor Y, required for in vitro synthesis of phiX174 DNA.

Authors: S Wickner; J Hurwitz
Journal: Proc Natl Acad Sci U S A Date: 1975-09 Impact factor: 11.205

4. Stimulation of DNA polymerase by factors isolated from Novikoff hepatoma.

Authors: G S Probst; D M Stalker; D W Mosbaugh; R R Meyer
Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

5. Replication of E. coli duplex DNA in vitro. The separation of the DNA containing fractions of a lysate from the soluble enzymes and their complementation properties.

Authors: V Nüsslein; S Henke; L H Johnston
Journal: Mol Gen Genet Date: 1976-05-07

9. Evidence for two genetically distinct DNA primase activities specified by plasmids of the B and I incompatibility groups.

Authors: B P Dalrymple; G J Boulnois; B M Wilkins; E Orr; P H Williams
Journal: J Bacteriol Date: 1982-07 Impact factor: 3.490

10. Involvement of two protein factors and ATP in in vitro DNA synthesis catalyzed by DNA polymerase 3 of Escherichia coli.

Authors: J Hurwitz; S Wickner
Journal: Proc Natl Acad Sci U S A Date: 1974-01 Impact factor: 11.205