Literature DB >> 4165744

Primary stimulation and measurement of antibody production to sheep red blood cells in vitro.

W A Robinson, J Marbrook, E Diener.   

Abstract

A method for the primary stimulation and measurement of antibody production to sheep red blood cells in vitro has been described. In this system when mouse spleen cells are incubated with sheep erythrocytes large complement-dependent plaques are formed. The number and size of plaques increases from day 1 to day 3 of incubation with an average of 4.4 plaque areas per 1 x 10(6) cells plated at day 3. There is a linear relationship between the number of spleen cells plated and the number of plaques formed. Plaque formation is inhibited by colchicine, actinomycin D, and rabbit anti-mouse globulin. This system offers a possible means for the direct in vitro measurement of the number of cells in a population susceptible to antigenic stimulation by sheep erythrocytes.

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Year:  1967        PMID： 4165744      PMCID： PMC2138321          DOI： 10.1084/jem.126.2.347

Source DB:  PubMed          Journal:  J Exp Med        ISSN： 0022-1007            Impact factor:   14.307


  11 in total

1.  PREPARATION OF ANTISERA SPECIFIC FOR 6.6 S GAMMA-GLOBULINS, BETA 2A-GLOBULINS, GAMMA-1.-MACROGLOBULINS, AND FOR TYPE I AND II COMMON GAMMA-GLOBULIN DETERMINANTS.

Authors:  J L FAHEY; C MCLAUGHLIN
Journal:  J Immunol       Date:  1963-10       Impact factor: 5.422

2.  Plaque formation in agar by single antibody-producing cells.

Authors:  N K JERNE; A A NORDIN
Journal:  Science       Date:  1963-04-26       Impact factor: 47.728

3.  The growth of mouse bone marrow cells in vitro.

Authors:  T R Bradley; D Metcalf
Journal:  Aust J Exp Biol Med Sci       Date:  1966-06

4.  Focal antibody production by transferred spleen cells in irradiated mice.

Authors:  J H Playfair; B W Papermaster; L J Cole
Journal:  Science       Date:  1965-08-27       Impact factor: 47.728

5.  A transplantation assay for mouse cells responsive to antigenic stimulation by sheep erythrocytes.

Authors:  J C Kennedy; L Siminovitch; J E Till; E A McCulloch
Journal:  Proc Soc Exp Biol Med       Date:  1965-12

6.  Thymus-marrow cell combinations. Synergism in antibody production.

Authors:  H N Claman; E A Chaperon; R F Triplett
Journal:  Proc Soc Exp Biol Med       Date:  1966 Aug-Sep

7.  The proliferative capacity of antigen-sensitive precursors of hemolytic plaque-forming cells.

Authors:  J C Kennedy; J E Till; L Siminovitch; E A McCulloch
Journal:  J Immunol       Date:  1966-06       Impact factor: 5.422

8.  Immunization of normal mouse spleen cell suspensions in vitro.

Authors:  R I Mishell; R W Dutton
Journal:  Science       Date:  1966-08-26       Impact factor: 47.728

9.  Antibody formation in nonimmune mouse peritoneal cells after incubation in gum containing antigen.

Authors:  A E Bussard
Journal:  Science       Date:  1966-08-19       Impact factor: 47.728

10.  The effects of antigens and of phytohemagglutinin on rabbit spleen cell suspensions.

Authors:  G Harris; R J Littleton
Journal:  J Exp Med       Date:  1966-10-01       Impact factor: 14.307
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  10 in total

1.  Medical research in melbourne.

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Journal:  Br Med J       Date:  1968-01-20

2.  The effects of RES 'blockade' on antibody formation. IV. Inhibition of plaque-forming cells in spleen cultures treated with carbon particles.

Authors:  T Y Sabet; H Friedman
Journal:  Immunology       Date:  1970-11       Impact factor: 7.397

3.  Response of rat blood, spleen, and lymph node leucocytes to soluble and insoluble antigen.

Authors:  B S Rabin; N R Rose
Journal:  Immunology       Date:  1970-08       Impact factor: 7.397

4.  Strain differences in the immune response of mice. II. Responses by neonatal cells in irradiated adult hosts.

Authors:  J H Playfair
Journal:  Immunology       Date:  1968-12       Impact factor: 7.397

5.  Foci of proliferating antibody-producing cells in a primary immune response in vitro.

Authors:  J Marbrook
Journal:  Clin Exp Immunol       Date:  1968-05       Impact factor: 4.330

6.  Immune responses in vitro. I. Cellular requirements for the immune response by nonprimed and primed spleen cells in vitro.

Authors:  C W Pierce
Journal:  J Exp Med       Date:  1969-08-01       Impact factor: 14.307

7.  Antigens in immunity. XV. Ultrastructural features of antigen capture in primary and secondary lymphoid follicles.

Authors:  G J Nossal; A Abbot; J Mitchell; Z Lummus
Journal:  J Exp Med       Date:  1968-02-01       Impact factor: 14.307

8.  Cells involved in the immune response. VII. The demonstration, using allotypic markers, of antibody formation by irradiation-resistant cells of irradiated rabbits injected with normal allogeneic bone marrow cells and sheep erythrocytes.

Authors:  M Richter; N I Abdou
Journal:  J Exp Med       Date:  1969-06-01       Impact factor: 14.307

9.  In vitro induction of a primary response to the dinitrophenyl determinant.

Authors:  S Segal; A Globerson; M Feldman; J Haimovich; M Sela
Journal:  J Exp Med       Date:  1970-01-01       Impact factor: 14.307

10.  Cells involved in the immune response. XI. Identification of the antigen-reactive cell as the tolerant cell in the immunologically tolerant rabbit.

Authors:  N I Abdou; M Richter
Journal:  J Exp Med       Date:  1969-07-01       Impact factor: 14.307
  10 in total

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