Literature DB >> 4139707

Heterogeneity of HL-A antigen preparations is due to variable sialic acid content.

P Parham, R E Humphreys, M J Turner, J L Strominger.   

Abstract

Purified, papain-solubilized preparations of HL-A antigen from cultured human lymphoblastoid cells (HL-A2 and HL-A7,12 from RPMI 4265 cells and a mixture of HL-A3, W-25, HL-A12, and HL-A27 from IM-1 cells) show substantial charge heterogeneity in isoelectric focusing gels. This heterogeneity can be ascribed largely to variable numbers of sialic acid residues on each molecule. Neuraminidase (EC 3.2.1.18) treatment of the HL-A antigens as a function of time altered the band patterns in a manner demonstrating that up to three sialic acid residues are present on both first locus ("LA") and second locus ("Four") antigens. Neuraminidase treatment did not alter the specificity or specific activity of the purified antigens.

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Year:  1974        PMID： 4139707      PMCID： PMC434314          DOI： 10.1073/pnas.71.10.3998

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  13 in total

1.  The complete amino acid sequence of beta 2-microglobulin.

Authors:  B A Cunningham; J L Wang; I Berggård; P A Peterson
Journal:  Biochemistry       Date:  1973-11-20       Impact factor: 3.162

2.  The molecular basis of codominant expression of the histocompatibility-2 genetic region.

Authors:  S E Cullen; B D Schwartz; S G Nathenson; M Cherry
Journal:  Proc Natl Acad Sci U S A       Date:  1972-06       Impact factor: 11.205

3.  Papain-solubilized HL-A antigens from cultured human lymphocytes contain two peptide fragments.

Authors:  P Cresswell; M J Turner; J L Strominger
Journal:  Proc Natl Acad Sci U S A       Date:  1973-05       Impact factor: 11.205

4.  Basic structure of mouse histocompatibility antigens.

Authors:  M Hess; D A Davies
Journal:  Eur J Biochem       Date:  1974-01-03

5.  Transplantation antigens from human spleens.

Authors:  A R Sanderson; J R Batchelor
Journal:  Nature       Date:  1968-07-13       Impact factor: 49.962

6.  Analytical fractionation of plant and animal proteins by gel electrofocusing.

Authors:  C W Wrigley
Journal:  J Chromatogr       Date:  1968-08-27

7.  Human lymphocyte antigen reactivity modified by neuraminidase.

Authors:  E A Grothaus; M W Flye; E Yunis; D B Amos
Journal:  Science       Date:  1971-08-06       Impact factor: 47.728

8.  Multiple common properties of human beta2-microglobulin and the common portion fragment derived from HL-A antigen molecules.

Authors:  K Nakamuro; N Tanigaki; D Pressman
Journal:  Proc Natl Acad Sci U S A       Date:  1973-10       Impact factor: 11.205

9.  Highly purified papain-solubilized HL-A antigens contain beta2-microglobulin.

Authors:  P A Peterson; L Rask; J B Lindblom
Journal:  Proc Natl Acad Sci U S A       Date:  1974-01       Impact factor: 11.205

10.  The small subunit of HL-A antigens is beta 2-microglobulin.

Authors:  H M Grey; R T Kubo; S M Colon; M D Poulik; P Cresswell; T Springer; M Turner; J L Strominger
Journal:  J Exp Med       Date:  1973-12-01       Impact factor: 14.307
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  17 in total

1.  Evidence for tryosine peptide homologies in the HLA antigens system.

Authors:  C Cunningham-Rundles; C Jersild; A Svejgaard; B Dupont; R A Good
Journal:  Proc Natl Acad Sci U S A       Date:  1975-12       Impact factor: 11.205

2.  Structure of HLA antigens: amino-acid and carbohydrate compositions and NH2-terminal sequences of four antigen preparations.

Authors:  C Terhorst; P Parham; D L Mann; J L Strominger
Journal:  Proc Natl Acad Sci U S A       Date:  1976-03       Impact factor: 11.205

3.  Immunological and chemical purity of papain-solubilized HL-A antigens.

Authors:  P Parham; C Terhorst; H Herrmann; R E Humphreys; M D Waterfield; J L Strominger
Journal:  Proc Natl Acad Sci U S A       Date:  1975-04       Impact factor: 11.205

4.  Structural analysis of an HLA-B27 functional variant: identification of residues that contribute to the specificity of recognition by cytolytic T lymphocytes.

Authors:  M A Vega; A Ezquerra; S Rojo; P Aparicio; R Bragado; J A López de Castro
Journal:  Proc Natl Acad Sci U S A       Date:  1985-11       Impact factor: 11.205

5.  Structural analysis of HLA-A2.4 functional variant KNE. Implications for the mapping of HLA-A2-specific T-cell epitopes.

Authors:  N Doménech; A Ezquerra; R Castaño; J A López de Castro
Journal:  Immunogenetics       Date:  1988       Impact factor: 2.846

6.  A human thy-1-like antigen expressed on cultured leukemic T-cells.

Authors:  F Saji; N Tanigaki
Journal:  Immunogenetics       Date:  1982       Impact factor: 2.846

7.  Analysis of human class I antigens by two-dimensional gel electrophoresis. I. Polymorphism, evidence for additional (non-HLA-A, B, C) gene products, and identification of variant HLA-A, B antigens.

Authors:  R G Vasilov; A Hahn; H Mölders; J J van Rood; M Breuning; H L Ploegh
Journal:  Immunogenetics       Date:  1983       Impact factor: 2.846

8.  Qa-2 does not display structural genetic polymorphism detectable on isoelectric-focusing gels.

Authors:  J Michaelson; L Flaherty; B Hutchinson; H Yudkowitz
Journal:  Immunogenetics       Date:  1982       Impact factor: 2.846

9.  Carbohydrate moieties of rat MHC class I antigens.

Authors:  D N Misra; H W Kunz; T J Gill
Journal:  Immunogenetics       Date:  1987       Impact factor: 2.846

10.  Immunochemical analysis of glycosylated and nonglycosylated DLA class I antigens.

Authors:  M J van der Feltz; H L Ploegh
Journal:  Immunogenetics       Date:  1984       Impact factor: 2.846
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