Literature DB >> 323855

The amino acid sequence of beta-galactosidase of Escherichia coli.

A V Fowler, I Zabin.   

Abstract

The amino acid sequence of beta-galactosidase was determined. The protein contains 1021 amino acid residues in a single polypeptide chain. The subunit molecular weight calculated from the sequence is 116,248. The sequence determination, carried out mainly by conventional methods, was aided by complementation tests, by the use of termination mutant strains, and by a new immunochemical method. The five residue sequence Thr-Pro-His-Pro-Ala appears twice within the polypeptide chain, but no other striking homologous features are evident.

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Year:  1977        PMID： 323855      PMCID： PMC430818          DOI： 10.1073/pnas.74.4.1507

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  8 in total

1.  Amino acid sequence of beta-galactosidase. IV. Sequence of an alpha-complementing cyanogen bromide peptide, residues 3 to 92.

Authors:  K E Langley; A V Fowler; I Zabin
Journal:  J Biol Chem       Date:  1975-04-10       Impact factor: 5.157

2.  The application of 0.1 M quadrol to the microsequence of proteins and the sequence of tryptic peptides.

Authors:  A W Brauer; M N Margolies; E Haber
Journal:  Biochemistry       Date:  1975-07       Impact factor: 3.162

3.  Beta-galactosidase from termination and deletion mutant strains.

Authors:  M R Villarejo; I Zabin
Journal:  J Bacteriol       Date:  1974-10       Impact factor: 3.490

4.  The amino acid sequence of -galactosidase. II. Tryptic peptides of the maleylated protein and sequences of some tryptic peptides.

Authors:  A V Fowler
Journal:  J Biol Chem       Date:  1972-09-10       Impact factor: 5.157

5.  The amino acid sequence of -galactosidase. 3. The sequences of NH 2 - and COOH-terminal tryptic peptides.

Authors:  I Zabin; A V Fowler
Journal:  J Biol Chem       Date:  1972-09-10       Impact factor: 5.157

6.  Beta-galactosidase: alpha-complementation of a deletion mutant with cyanogen bromide peptides.

Authors:  S Lin; M Villarejo; I Zabin
Journal:  Biochem Biophys Res Commun       Date:  1970-07-27       Impact factor: 3.575

7.  The amino acid sequence of beta galactosidase. I. Isolation and composition of tryptic peptides.

Authors:  A V Fowler; I Zabin
Journal:  J Biol Chem       Date:  1970-10-10       Impact factor: 5.157

8.  Co-linearity of beta-galactosidase with its gene by immunological detection of incomplete polypeptide chains.

Authors:  A V Fowler; I Zabin
Journal:  Science       Date:  1966-11-25       Impact factor: 47.728
  8 in total
  57 in total

1.  Sites within gene lacZ of Escherichia coli for formation of active hybrid beta-galactosidase molecules.

Authors:  E Brickman; T J Silhavy; P J Bassford; H A Shuman; J R Beckwith
Journal:  J Bacteriol       Date:  1979-07       Impact factor: 3.490

2.  beta-Galactosidase chimeras: primary structure of a lac repressor-beta-galactosidase protein.

Authors:  A J Brake; A V Fowler; I Zabin; J Kania; B Müller-Hill
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

3.  Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control. II. DNA fragment insertion at the vicinity of the lac UV5 promoter.

Authors:  P Charnay; A Louise; A Fritsch; D Perrin; P Tiollais
Journal:  Mol Gen Genet       Date:  1979-02-26

4.  Phosphorylation of the C-terminal domain of the Na+/H+ exchanger by Ca2+/calmodulin-dependent protein kinase II.

Authors:  L Fliegel; M P Walsh; D Singh; C Wong; A Barr
Journal:  Biochem J       Date:  1992-02-15       Impact factor: 3.857

5.  Regulated expression by readthrough translation from a plasmid-encoded beta-galactosidase.

Authors:  P O'Farrell; B Polisky; D H Gelfand
Journal:  J Bacteriol       Date:  1978-05       Impact factor: 3.490

6.  Functional properties of beta-galactosidase from mutant strain 13 PO of Escherichia coli.

Authors:  P J Deschavanne; O M Viratelle; J M Yon
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

7.  Evidence for in vivo ribosome recycling, the fourth step in protein biosynthesis.

Authors:  L Janosi; S Mottagui-Tabar; L A Isaksson; Y Sekine; E Ohtsubo; S Zhang; S Goon; S Nelken; M Shuda; A Kaji
Journal:  EMBO J       Date:  1998-02-16       Impact factor: 11.598

8.  Human T cell responses to beta-galactosidase.

Authors:  J D Oxley; R H Brookes; L S Rayfield; P S Shepherd
Journal:  Clin Exp Immunol       Date:  1991-03       Impact factor: 4.330

9.  The catalytic consequences of experimental evolution. Studies on the subunit structure of the second (ebg) beta-galactosidase of Escherichia coli, and on catalysis by ebgab, an experimental evolvant containing two amino acid substitutions.

Authors:  A C Elliott; S K; M L Sinnott; P J Smith; J Bommuswamy; Z Guo; B G Hall; Y Zhang
Journal:  Biochem J       Date:  1992-02-15       Impact factor: 3.857

10.  Viral sequestration of antigen subverts cross presentation to CD8(+) T cells.

Authors:  Eric F Tewalt; Jean M Grant; Erica L Granger; Douglas C Palmer; Neal D Heuss; Dale S Gregerson; Nicholas P Restifo; Christopher C Norbury
Journal:  PLoS Pathog       Date:  2009-05-29       Impact factor: 6.823
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