Literature DB >> 4124305

Quantitative deficiency of chain-specific globin messenger ribonucleic acids in the thalassemia syndromes.

D Housman, B G Forget, A Skoultchi, E J Benz.   

Abstract

A hybridization assay procedure was devised that makes possible quantitation of the ratio of mRNA of alpha to mRNA of beta globin chains in an RNA sample. The assay uses the radioactive synthetic DNA copies obtained by incubation of RNA-dependent DNA polymerase of avian myeloblastosis virus with rabbit globin mRNA that is 80-90% enriched in mRNA specific for synthesis of alpha or beta globin chains. The rabbit alpha-chain mRNA is obtained from the postribosomal supernatant of rabbit reticulocyte lysates; the rabbit beta-chain mRNA is obtained from the largest polysomes of rabbit reticulocytes treated with L-O-methylthreonine. Sufficient homology exists between rabbit and human globin chains and globin mRNAs that the synthetic DNA copies of chain-specific rabbit globin mRNA hybridize with human globin mRNA. Applied to the study of globin mRNA isolated from reticulocytes of humans with alpha and beta thalassemia, the technique revealed marked quantitative deficiency of alpha-chain mRNA relative to beta-chain mRNA in alpha thalassemia and similar deficiency of beta-chain mRNA relative to alpha-chain mRNA in beta thalassemia. The thalassemia syndromes are therefore characterized by true quantitative deficiency of the mRNA specific for the affected globin chain.

Entities:  

Mesh：

Substances：

Year:  1973        PMID： 4124305      PMCID： PMC433602          DOI： 10.1073/pnas.70.6.1809

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  32 in total

1.  Association of an endoribonuclease with the avian myeloblastosis virus deoxyribonucleic acid polymerase.

Authors:  D Baltimore; D F Smoler
Journal:  J Biol Chem       Date:  1972-11-25       Impact factor: 5.157

2.  In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA.

Authors:  I M Verma; G F Temple; H Fan; D Baltimore
Journal:  Nat New Biol       Date:  1972-02-09

3.  A nuclease specific for heat-denatured DNA in isolated from a product of Aspergillus oryzae.

Authors:  T Ando
Journal:  Biochim Biophys Acta       Date:  1966-01-18

4.  Messenger RNA for globin in the postribosomal supernatant of rabbit reticulocytes.

Authors:  M Jacobs-Lorena; C Baglioni
Journal:  Proc Natl Acad Sci U S A       Date:  1972-06       Impact factor: 11.205

5.  Haemoglobin synthesis in beta-thalassaemia.

Authors:  J B Clegg; D J Weatherall; S Na-Nakorn; P Wasi
Journal:  Nature       Date:  1968-11-16       Impact factor: 49.962

6.  Beta thalassemia and translation of globin messenger RNA.

Authors:  D G Nathan; H F Lodish; Y W Kan; D Housman
Journal:  Proc Natl Acad Sci U S A       Date:  1971-10       Impact factor: 11.205

7.  [Free alpha-hemoglobin and hemoglobin biosynthesis].

Authors:  N Blum; N Maleknia; G Schapira
Journal:  Biochim Biophys Acta       Date:  1969-04-22

8.  Inhibition of the biosynthetic completion of rabbit hemoglobin by isolated human hemoglobin chains.

Authors:  J R Shaeffer; P K Trostle; R F Evans
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

9.  Changing rates of globin chain synthesis during erythroid cell maturation in thalassemia.

Authors:  A S Braverman; A Bank
Journal:  J Mol Biol       Date:  1969-05-28       Impact factor: 5.469

10.  Defect in messenger RNA for human hemoglobin synthesis in beta thalassemia.

Authors:  E J Benz; B G Forget
Journal:  J Clin Invest       Date:  1971-12       Impact factor: 14.808
View more
  40 in total

1.  Globin synthesis in fractionated Normoblasts of beta-thalassemia heterozygotes.

Authors:  W G Wood; G Stamatoyannopoulos
Journal:  J Clin Invest       Date:  1975-03       Impact factor: 14.808

2.  Nucleotide sequences of the 3'-terminal untranslated region of messenger RNA for human beta globin chain.

Authors:  B G Forget; C A Marotta; S M Weissman; M Cohen-Solal
Journal:  Proc Natl Acad Sci U S A       Date:  1975-09       Impact factor: 11.205

3.  On the defect of synthesis ceruloplasmin in the liver polyribosomes in Wilson's disease.

Authors:  V S Gaitskhoki; O I Kisselev; K A Moshkov; L V Puchkova; M M Shavlovski; V S Shulman; V G Vacharlovski; S A Neifakh
Journal:  Biochem Genet       Date:  1975-10       Impact factor: 1.890

4.  The thalassemias and related disorders.

Authors:  Alain J Marengo-Rowe
Journal:  Proc (Bayl Univ Med Cent)       Date:  2007-01

5.  beta (+)-Thalassaemia in the Po river delta region (northern Italy): genotype and beta globin synthesis.

Authors:  L Del Senno; M Pirastu; R Barbieri; F Bernardi; D Buzzoni; G Marchetti; C Perrotta; C Vullo; Y W Kan; F Conconi
Journal:  J Med Genet       Date:  1985-02       Impact factor: 6.318

6.  Molecular evolution of human and rabbit beta-globin mRNAs.

Authors:  F C Kafatos; A Efstratiadis; B G Forget; S M Weissman
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

Review 7.  The molecular basis of disorders of human hemoglobin synthesis.

Authors:  F Ramirez; J G Mears; A Bank
Journal:  Mol Cell Biochem       Date:  1980-08-16       Impact factor: 3.396

8.  Molecular analysis of the beta-thalassemia phenotype associated with inheritance of hemoglobin E (alpha 2 beta2(26)Glu leads to Lys).

Authors:  E J Benz; B W Berman; B L Tonkonow; E Coupal; T Coates; L A Boxer; A Altman; J G Adams
Journal:  J Clin Invest       Date:  1981-07       Impact factor: 14.808

9.  Insertion of synthetic copies of human globin genes into bacterial plasmids.

Authors:  J T Wilson; L B Wilson; J K deRiel; L Villa-komaroff; A Efstratiadis; B G Forget; S M Weissman
Journal:  Nucleic Acids Res       Date:  1978-02       Impact factor: 16.971

10.  Nucleotide sequences of human globin messenger RNA.

Authors:  C A Marotta; B G Forget; S M Weissman; I M Verma; R P McCaffrey; D Baltimore
Journal:  Proc Natl Acad Sci U S A       Date:  1974-06       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.