Literature DB >> 411684

Purification and immobilization of human carbonic anhydrase B by using polyacrylamide gel.

B Ray.   

Abstract

Human erythrocyte carbonic anhydrase B was purified and immobilized in polyacrylamide gel. As compared to the soluble enzyme, the immobilized enzyme was considerably more resistant to heat and sulphanilamide action.

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Year:  1977        PMID： 411684     DOI： 10.1007/bf01918793

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  9 in total

1.  Affinity chromatography of carbonic anhydrase.

Authors:  S O. Falkbring; P O. Göthe; P O. Nyman; L Sundberg; J Porath
Journal:  FEBS Lett       Date:  1972-08-01       Impact factor: 4.124

2.  SEMIPERMEABLE MICROCAPSULES.

Authors:  T M CHANG
Journal:  Science       Date:  1964-10-23       Impact factor: 47.728

3.  "Molecular-sieve" chromatography of proteins on colums of cross-linked polyacrylamide.

Authors:  S HJERTEN; R MOSBACH
Journal:  Anal Biochem       Date:  1962-02       Impact factor: 3.365

4.  Acetyl chymotrypsin and its reaction with ethanol.

Authors:  A K BALLS; H N WOOD
Journal:  J Biol Chem       Date:  1956-03       Impact factor: 5.157

5.  The manometric determination of the activity of carbonic anhydrase under varied conditions.

Authors:  F J Roughton; V H Booth
Journal:  Biochem J       Date:  1946       Impact factor: 3.857

6.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

Review 7.  Affinity chromatography of macromolecules.

Authors:  P Cuatrecasas
Journal:  Adv Enzymol Relat Areas Mol Biol       Date:  1972

8.  Covalent linkage of functional groups, ligands, and proteins to polyacrylamide beads.

Authors:  J K Inman
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

9.  Enzymes bound to artificial matrixes.

Authors:  K Mosbach
Journal:  Sci Am       Date:  1971-03       Impact factor: 2.142
  9 in total

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