Literature DB >> 410004

Antibodies specific to a deoxyribodinucleotide sequence.

S A Khan, M Z Humayun, T M Jacob.   

Abstract

Antibodies were raised in rabbits against the bovine serum albumin conjugate of dpApT. Analysis by double diffusion in agar gel and quantitative precipitation test showed the presence of antibodies specific to the hapten in the antisera. Quantitative data on the specificity of the antibodies were obtained by studying the inhibition of the binding of 3H-dpApT to the antisera by various nonradioactive mono- and oligonucleotides, using a nitrocellulose membrane binding assay. The antibodies were found to be highly specific for the dinucleotide sequence dpApT. The antibodies were able to bind to synthetic oligonucleotides containing the sequence dpApT and to denatured calf thymus DNA.

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Year:  1977        PMID： 410004      PMCID： PMC342629          DOI： 10.1093/nar/4.9.2997

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  14 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Completed DNA sequences and organization of repressor-binding sites in the operators of phage lambda.

Authors:  Z Humayun; A Jeffrey; M Ptashne
Journal:  J Mol Biol       Date:  1977-05-15       Impact factor: 5.469

3.  Antibodies to the codons ApApAp, ApApC, and ApUpG.

Authors:  R M D'Alisa; B F Erlanger
Journal:  Biochemistry       Date:  1974-08-13       Impact factor: 3.162

4.  Specificity of anti-nucleoside antibodies.

Authors:  M Z Humayun; T M Jacob
Journal:  Biochem J       Date:  1974-07       Impact factor: 3.857

5.  The production of antibodies to mononucleotides, oligonucleotides and DNA.

Authors:  M J Halloran; C W Parker
Journal:  J Immunol       Date:  1966-03       Impact factor: 5.422

6.  Specific antibodies to dinucleotides and trinucleotides.

Authors:  B Bonavida; S Fuchs; M Sela; P W Roddy; H A Sober
Journal:  Eur J Biochem       Date:  1972-12-18

7.  Specificity of antibodies in sera of rabbits immunized with dinucleotide-egg albumin conjugates.

Authors:  S Estrada-Parra; E García-Ortigoza
Journal:  Immunochemistry       Date:  1972-08

8.  High resolution detection of DNA-RNA hybrids in situ by indirect immunofluorescence.

Authors:  G T Rudkin; B D Stollar
Journal:  Nature       Date:  1977-02-03       Impact factor: 49.962

9.  Immunologic studies on nucleic acids and their components. I. An analysis of the specificity of anti-deoxyadenylate antibodies by a membrane-binding technique.

Authors:  M Z Humayun; T M Jacob
Journal:  Biochim Biophys Acta       Date:  1973-11-26

10.  Ribosome structure: localization of N6,N6-dimethyladenosine by electron microscopy of a ribosome-antibody complex.

Authors:  S M Politz; D G Glitz
Journal:  Proc Natl Acad Sci U S A       Date:  1977-04       Impact factor: 11.205
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  4 in total

1.  Formation and characterization of antibody against 2'-(5"-phosphoribosyl)-5' AMP, the monomer form of poly(adenosine diphosphate ribose).

Authors:  H Sakura; M Miwa; Y Kanai; T Matsushima; T Sugimura
Journal:  Nucleic Acids Res       Date:  1978-11       Impact factor: 16.971

2.  Base specific binding of deoxyguanylate and deoxycytidylate antibodies to double stranded DNA.

Authors:  A Jacob; T M Jacob
Journal:  Nucleic Acids Res       Date:  1982-10-25       Impact factor: 16.971

3.  Antibodies elicited by defined oligodeoxyribonucleotide sequences.

Authors:  H J Störl; H Simon; H Barthelmes
Journal:  Nucleic Acids Res       Date:  1978-12       Impact factor: 16.971

4.  Monoclonal antibodies showing sequence specificity in their interaction with single-stranded DNAs.

Authors:  J S Lee; J R Lewis; A R Morgan; T R Mosmann; B Singh
Journal:  Nucleic Acids Res       Date:  1981-04-10       Impact factor: 16.971
  4 in total

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