Literature DB >> 4055031

Identification of precursor of phagocytosis-stimulating factor in guinea pig polymorphonuclear neutrophils.

Y Ishibashi, T Yamashita.   

Abstract

A precursor of phagocytosis-stimulating factor (PSF) was identified by immunoblot assay with purified anti-PSF antibodies. The purified anti-PSF antibodies recognized not only the PSF with a molecular weight of 16,000 (16K) but also the 36K protein with a pI of 6.5 in the granule fraction of polymorphonuclear neutrophils, indicating that this 36K protein has an antigenic determinant common to PSF. In addition, the appearance of the PSF during phagocytosis by polymorphonuclear neutrophils was closely correlated to the decrease of the 36K protein. These results suggest that the 36K protein is the precursor of PSF which is converted to biologically active PSF in the granules during phagocytosis.

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Year:  1985        PMID： 4055031      PMCID： PMC261982          DOI： 10.1128/iai.50.2.500-505.1985

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  18 in total

1.  The sulfhydryl groups involved in the active site of myosin B adenosinetriphosphatase. V. The possible localization of Sa and other sulfhydryl groups in myosin rods.

Authors:  T Horigome; T Yamashita
Journal:  J Biochem       Date:  1979-01       Impact factor: 3.387

2.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

3.  Characterization of lymphocyte-activating factor (LAF) produced by human mononuclear cells: biochemical relationship of high and low molecular weight forms of LAF.

Authors:  A Togawa; J J Oppenheim; S B Mizel
Journal:  J Immunol       Date:  1979-05       Impact factor: 5.422

4.  Generation of a phagocytosis-stimulating factor by polymorphonuclear neutrophils during phagocytosis.

Authors:  Y Ishibashi; T Yamashita
Journal:  Int Arch Allergy Appl Immunol       Date:  1981

5.  Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.

Authors:  J B Olmsted
Journal:  J Biol Chem       Date:  1981-12-10       Impact factor: 5.157

6.  Inactivation during phagocytosis of leucine aminopeptidase, an ecto-enzyme of polymorphonuclear neutrophils.

Authors:  I Nagaoka; T Yamashita
Journal:  Biochim Biophys Acta       Date:  1981-12-18

7.  Effect of endocellular cryoprotectant upon polymorphonuclear neutrophil function during storage at low temperature.

Authors:  T Yamashita; N Imaizumi; S Yuasa
Journal:  Cryobiology       Date:  1979-04       Impact factor: 2.487

8.  Porcine proinsulin: characterization and amino acid sequence.

Authors:  R E Chance; R M Ellis; W W Bromer
Journal:  Science       Date:  1968-07-12       Impact factor: 47.728

9.  Purification and characterization of a phagocytosis-stimulating factor from phagocytosing polymorphonuclear neutrophils: comparison with granule basic proteins.

Authors:  Y Ishibashi; T Yamashita
Journal:  Infect Immun       Date:  1985-06       Impact factor: 3.441

10.  Evidence for pro-beta-nerve growth factor, a biosynthetic precursor to beta-nerve growth factor.

Authors:  E A Berger; E M Shooter
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205
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  1 in total

1.  Effects of a phagocytosis-stimulating factor derived from polymorphonuclear neutrophils on the functions of macrophages.

Authors:  Y Ishibashi; T Yamashita
Journal:  Infect Immun       Date:  1987-08       Impact factor: 3.441
  1 in total

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